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Rhizoma atractylodis macrocephalae polysaccharide capable of resisting alcoholic liver injury as well as preparation method and application of rhizoma atractylodis macrocephalae polysaccharide

An Atractylodes Polysaccharide, liver injury technology, applied in the direction of resisting vector-borne diseases, medical preparations containing active ingredients, pharmaceutical formulations, etc. The effect of reducing and alleviating liver damage and inhibiting the increase of liver index

Active Publication Date: 2021-07-30
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature research found that there was no research on the protective effect of Atractylodes macrocephala polysaccharide on alcoholic liver injury

Method used

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  • Rhizoma atractylodis macrocephalae polysaccharide capable of resisting alcoholic liver injury as well as preparation method and application of rhizoma atractylodis macrocephalae polysaccharide
  • Rhizoma atractylodis macrocephalae polysaccharide capable of resisting alcoholic liver injury as well as preparation method and application of rhizoma atractylodis macrocephalae polysaccharide
  • Rhizoma atractylodis macrocephalae polysaccharide capable of resisting alcoholic liver injury as well as preparation method and application of rhizoma atractylodis macrocephalae polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation method of embodiment 1 Atractylodes macrocephala polysaccharide

[0038] 1. Atractylodes macrocephala crude polysaccharide extraction:

[0039] Take Atractylodes macrocephala, crush it, add 15 times the amount of water to extract 3 times, each time for 1 hour, combine the extracts, concentrate, to obtain a concentrated solution, add ethanol to make the final concentration of ethanol in the system 80%, set aside for precipitation, filter or centrifuge, Dried to obtain the crude polysaccharide of Atractylodes macrocephala;

[0040] 2. Macroporous resin decolorization:

[0041] Take step (1) Atractylodes Rhizome crude polysaccharide, add water to dissolve, obtain concentration 25mg / mL Atractylodes Rhizome crude polysaccharide solution, adjust the pH value of polysaccharide solution to 6, add HPD600 macroporous resin column, place in a shaker, 125r / min, at temperature Decolorize at 75°C, decolorize for 6 hours, centrifuge at 3000r / min for 3 minutes, take the...

Embodiment 2

[0055] Example 2 Structural Identification of Atractylodes Rhizoma Polysaccharides

[0056] 1. Purity identification and relative molecular mass determination

[0057] 1. Materials and methods

[0058] 1.1 Materials and Instruments

[0059] Atractylodes polysaccharide (BZJP) prepared in Example 1, dextran molecular weight standard set (10 kinds), including dextran DO Mp180, dextran D1 Mp2700, dextran D2 Mp5250, dextran D3 Mp9750, dextran D4Mp13050, dextran D5 Mp36800, dextran D6 Mp64650, dextran D7 Mp135350, Dextran D8Mp300600, Dextran D2000 Mp2000000 (China Institute for Food and Drug Control); Coomassie Brilliant Blue G-250, bovine serum albumin, phenol, sulfuric acid, phosphoric acid, etc. are domestic analytically pure.

[0060] 101-00B type electric heating constant temperature drying oven (Luyue Instrument and Equipment Factory, Shangyu District, Shaoxing City); Agilent 1260 high performance liquid chromatography (Agilent Technologies Co., Ltd.).

[0061] 1.2 Experime...

Embodiment 3

[0115] Example 3 Study on the Protective Effect of Atractylodes Polysaccharides on Acute Alcoholic Liver Injury in Mice

[0116] 1. Materials and methods

[0117] 1.1 Materials and Instruments

[0118] 1.1.1 Experimental animals:

[0119] Clean-grade Kunming mice, male, weighing (20±2) g, were purchased from Qinglong Mountain. The temperature of the breeding environment is (23±2)°C, the humidity is 40%-60%, and the cycle of alternating light and dark is 12h.

[0120] 1.1.2 Drugs and reagents

[0121] Atractylodes macrocephala polysaccharide BZTP (prepared in Example 1); absolute ethanol (Sinopharm Group Co., Ltd); aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total protein quantitative assay (BCA method) kit (Nanjing Jiancheng Bioengineering Institute).

[0122] 1.1.3 Instruments

[0123] Tissuelyser-48 automatic sample rapid grinding instrument (Sha...

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Abstract

The invention discloses rhizoma atractylodis macrocephalae polysaccharide capable of resisting alcoholic liver injury and a preparation method of the rhizoma atractylodis macrocephalae polysaccharide. The rhizoma atractylodis macrocephalae polysaccharide is prepared from glucose, mannose and xylose in a molar ratio of 6.8:3.0:1. The molecular weight of the rhizoma atractylodis macrocephalae polysaccharide is 5465 Da. The primary structure of the rhizoma atractylodis macrocephalae polysaccharide is [beta]Xylp-[(1->3)-[beta]Xylp]6-(1->6)-[alpha]Glcp-[(1->4)-[alpha]Glcp]40-[(1->2)-[alpha]Manp]12. Experimental results show that the rhizoma atractylodis macrocephalae polysaccharide has an obvious protection effect on acute alcoholic liver injury of mice, can obviously inhibit the increase of the activity of AST, ALT and TG in serum caused by the acute alcoholic liver injury, effectively inhibits the increase of liver indexes, the increase of MDA content in the liver and the reduction of the activity of SOD and GSH-Px caused by alcohol intake, and can effectively relieve the alcoholic liver injury symptom of the mouse, and it is shown that the rhizoma atractylodis macrocephalae polysaccharide has a relatively good protection effect on the ethanol-induced acute liver injury of the mouse.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to an anti-alcoholic liver injury Atractylodes macrocephala polysaccharide and a preparation method thereof. Background technique [0002] Alcoholic liver disease (Alcoholic Hepatitis) is a liver disease caused by long-term heavy drinking. The initial stage is usually fatty liver, which can develop into alcoholic hepatitis, liver fibrosis and cirrhosis, and even liver cancer. The morbidity and mortality of alcoholic liver disease are increasing year by year, and it has become a global public health problem. At present, the number of deaths due to alcoholism is about 2.5 million in the world every year, accounting for 4% of the total mortality rate, surpassing AIDS and becoming the eighth leading cause of death. Alcohol has become the second largest cause of liver damage after hepatitis virus. Bring heavy economic burden to individual, family and society. In China, there has not been a na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
CPCC08B37/006C08B37/0003A61K31/715A61P1/16Y02A50/30
Inventor 王令充李俊松狄留庆杨颖嵇晶
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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