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Function and application of FGF21 gene in treatment of acute drug-induced liver injury

A drug-induced liver injury, FGF21 technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems affecting acute chemical liver injury and achieve the effect of relieving liver injury

Inactive Publication Date: 2019-04-12
张淑敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on previous research results, we believe that the anti-oxidative stress effect of FGE21 may affect the occurrence and development of acute chemical liver injury, and FGF21 may have a protective effect on acute chemical liver injury

Method used

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  • Function and application of FGF21 gene in treatment of acute drug-induced liver injury

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Experimental animals and feeding:

[0020] Species, age, sex and source of experimental animals: FGF21 knockout mice were purchased from JAX Laboratory in the United States, and bred and raised in the Experimental Animal Center of our university. Eight-week-old male FGF21 knockout mice and littermate wild-type control mice (16 each) were used in this experiment. Alternate lighting every 12 hours, temperature 20±2℃

Embodiment 2

[0022] Experimental grouping and methods: 1) WT group: 8-week-old male C57BL / 6J mice, without any treatment; 2) FGF21 KO group: 8-week-old male FGF21 KO mice, 8 without any treatment ;3) WT+HFD group: 8-week-old male C57BL / 6J mice, 8 mice, intraperitoneally injected carbon tetrachloride 150 mg / kg; 4) FGF 21 KO+HFD group: 8-week-old male FGF21 KO mice, 8 Only, intraperitoneal injection of carbon tetrachloride 150mg / kg. After 24 hours of induction, the animals in the above groups were anesthetized and sacrificed, and their eyeballs were removed to collect blood. After standing still for 2 hours, they were centrifuged at 3000 rpm for 10 minutes, and the supernatant serum was collected. Indicators were detected using commercial kits (built in Nanjing), and relevant operations followed the kit instructions.

[0023] When acute chemical liver injury occurs, a large number of liver cells are necrotic, and a large amount of alanine aminotransferase will be released into the blood, re...

Embodiment 3

[0025] Experimental grouping and methods: 1) WT group: 8-week-old male C57BL / 6J mice, without any treatment; 2) FGF21 KO group: 8-week-old male FGF21 KO mice, 8 without any treatment ;3) WT+HFD group: 8-week-old male C57BL / 6J mice, 8 mice, intraperitoneally injected carbon tetrachloride 150 mg / kg; 4) FGF 21 KO+HFD group: 8-week-old male FGF21 KO mice, 8 Only, intraperitoneal injection of carbon tetrachloride 150mg / kg. After 24 hours of induction, the animals in the above groups were anesthetized and sacrificed, and their eyeballs were removed to collect blood. After standing still for 2 hours, they were centrifuged at 3000 rpm for 10 minutes, and the supernatant serum was collected. Indicators were detected using commercial kits (built in Nanjing), and relevant operations followed the kit instructions.

[0026] When acute chemical liver injury occurs, a large number of liver cells are necrotic, and a large amount of aspartate aminotransferase will be released into the blood, ...

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Abstract

The present invention discloses an application of fibroblast growth factor 21 (FGF21) gene in prevention and treatment of acute chemical liver injury. The present invention firstly determines a relationship between the FGF21 gene and the acute chemical liver injury. FGF21 gene knockout can aggravate the acute chemical liver injury induced by carbon tetrachloride, mainly reflected in that the FGF21gene knockout can aggravate carbon tetrachloride-induced serum glutamic-pyruvic transaminase and glutamic oxalacetic transaminase level elevation, which indicates a function of the FGF21 gene in theacute chemical liver injury and is mainly reflected in that the FGF21 has functions of lowering the serum glutamic-pyruvic transaminase and glutamic oxalacetic transaminase levels. Aiming at the functions of the FGF21 gene, the FGF21 can be used as a drug for prevention, alleviation and / or treatment of the acute chemical liver injury; the FGF21 can be used as a drug target used for screening drugsfor the prevention, alleviation and / or treatment of the acute chemical liver injury; and the FGF21 can also be used as a target gene in gene therapy used for design and preparation of drugs and / or biological agents used for the prevention, alleviation and / or treatment of acute drug-induced liver injury.

Description

technical field [0001] The invention belongs to the field of biological treatment technology and genetic engineering medicine. It specifically relates to the function and application of a FGF21 gene in the treatment of acute drug-induced liver injury. Background technique [0002] Chemical liver injury is liver injury caused by chemical hepatotoxic substances. These chemicals include alcohol, toxic chemicals in the environment, and certain drugs. As an important detoxification organ of the human body - the liver has dual blood supply from the hepatic artery and the hepatic vein. Chemicals can enter the liver through the portal vein of the gastrointestinal tract or the systemic circulation for transformation, so the liver is vulnerable to toxic substances in chemicals. There are some substances that are toxic to the liver in both nature and human industrial production, which are called "liver-friendly poisons". These poisons are generally susceptible to the human populatio...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K38/18A61P1/16
CPCA61K38/1825A61K45/00A61P1/16
Inventor 张淑敏
Owner 张淑敏
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