Preparation process of raspberry tea and semen cassiae solid beverage and application of raspberry tea and semen cassiae solid beverage in liver protection
A technology of cassia seed and berry tea, applied to medical preparations containing active ingredients, plant raw materials, medical formulas, etc., to achieve the effect of improving liver function
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Embodiment 1
[0083] Prepare berry tea cassia solid beverage (such as figure 1 shown):
[0084] 100kg of qualified raspberry tea raw materials are crushed into 10 mesh, sieved (10 mesh screen), pure water reflux extraction is to weigh 100kg of raw materials, and 1 ton of pure water is heated between 75 ℃ - 80 ℃, 1 hour / time x 2 times (continuous reflux through centrifugal pump extraction of feed liquid to keep the material in the extraction tank at high temperature), vacuum concentration (-0.02Mp-0.095Mp, <60°C-80°C, concentrated to 40% solids) , this example adopts the conditions of -0.05Mp and 70°C. Then carry out spray drying (LPG50 drying tower, inlet air temperature 165°C, outlet air temperature 75°C-85°C, atomizing motor frequency 300Hz-400Hz, feeding frequency 25Hz), drying to moisture <5%, and then 100% drying An 80-mesh shaking sieve was used to obtain the berry tea extract for later use.
[0085] According to the same process, extract the cassia seed extract for use.
[0086...
Embodiment 2
[0088] 1 Materials and methods
[0089] 1.1 Main reagents and instruments
[0090]Reagents: MEM, Zhongqiao Xinzhou; FBS (fetal bovine serum), Saiye Biotechnology; double antibodies (penicillin, streptomycin), EDTA, Beijing Coollab Technology Co., Ltd.; RNA lysis buffer, reverse transcription kit, fluorescence quantitative PCR kit (Vazyme); alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tumor necrosis factor (TNF-α), interleukin 6 (IL-6), triglyceride (TG) ), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), lipopolysaccharide (LPS) ELISA detection kit, Changsha Aoji Biotechnology Co., Ltd.; D-hanks water ;43° Niulanshan aging.
[0091] Instrument: CO 2 Incubator, constant temperature water bath (Shanghai Yiheng), ultra-clean workbench (Thermo), ultra-low temperature refrigerator (Thermo Company, USA), refrigerated centrifuge (Hettich MIKRO-22R), inverted microscope (ZEISS), multi-function microplate...
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