Continuous gradient bionic manufacturing method based on pneumatic accurate control active cartilage scaffold

Abstract

The invention provides a continuous gradient bionic manufacturing method based on a pneumatic accurate control active cartilage scaffold. Based on sodium alginate SA ionic crosslinking and gelatin Gel low-temperature solidification forming mechanisms, SA, Gel, hydroxyapatite HA and cartilage cell suspension liquid are used as scaffold base materials, and according to the morphological characteristics of natural soft tissues, an extrusion deposition 3D printing technology based on high-pressure controllable gas is adopted to realize the preparation of an embedded living cell three-dimensional soft scaffold; and in the preparation process, the pressure applied to different charging barrels is dynamically and accurately regulated and controlled in real time, so that the accurate controllability of the component content is realized, then real-time dynamic mixing and continuous gradient laying of different component materials are realized through a dynamic mixing spray head, and the scaffold is crosslinked under the combined action of a CaCl2 solution and a low-temperature platform. The problems that an existing cartilage scaffold material is single in concentration, and the single material components of each layer in a layered structure do not conform to the anatomical characteristics of the continuous gradient of natural cartilage are solved.

Description

technical field [0001] The invention belongs to the field of cartilage tissue engineering, and in particular relates to a continuous gradient bionic manufacturing method based on pneumatic precise control of an active cartilage support. Background technique [0002] Articular cartilage defect is one of the common clinical diseases of bone and joint. Due to the poor proliferation ability of chondrocytes, the wound cannot be repaired by self-proliferation of chondrocytes after injury, resulting in irreversible cartilage defects caused by various injuries, inflammation and degenerative diseases. There are two main traditional treatment options, one is autologous bone transplantation, but it will cause new trauma and easily lead to donor tissue complications; Risk of infectious disease. In recent years, the emergence of 3D printed tissue-engineered cartilage scaffolds has brought hope for the repair of clinical articular cartilage defects. [0003] The current 3D printing tech...

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Problems solved by technology

[0010] In order to overcome the problem that the concentration of the existing cartilage scaffold material is single, the material composition of each layer in the layered structure is single, etc., which are inconsistent with the anatomical characteristics of the continuous gradient of natural cartilage, the present invention proposes a solution of SA, Gel, HA, chondrocyte suspension As a cartilage scaffold matrix material, a method for preparing a continuous gradient active cartilage scaffold based on the formation mechanism of sodium alginate ion cross-linking and gelatin low-temperature solidification: first prepare SA, Gel and SA / HA hydrogel and chondrocyte suspension, and use precise The controllable high-pressure gas exerts dynamically changing pressure on the barrels containing different printing materials, and then realizes real-time dynamic mixing and continuous gradient laying of different component materials through the dynamic mixing nozzle. 2 Under the joint action of the solution and the low-temperature platform, the cross-linking is finalized, and the layer-by-layer stacking finally completes the continuous gradient biomimetic manufacturing of the active cartilage scaffold embedded with cells

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