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A kit for detecting immunoglobulin g in urine

An immunoglobulin and kit technology, used in biological testing, measuring devices, material testing products, etc., can solve problems such as affecting the accuracy of test results, precipitation, and antibody instability, eliminating the need for filtration steps and simple operation steps. , the effect of improving antibody stability

Active Publication Date: 2022-03-29
BEIJING DIAGREAT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that the antibody in the existing immunoturbidimetric reagent is unstable and easy to precipitate and affect the accuracy of the test result, this application provides a kit for detecting immunoglobulin G in urine

Method used

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  • A kit for detecting immunoglobulin g in urine
  • A kit for detecting immunoglobulin g in urine
  • A kit for detecting immunoglobulin g in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kit for detecting immunoglobulin G in urine, comprising reagent R1 and reagent R2;

[0050] The configuration method of reagent R1 is: weigh 2.42g of Tris base, 40g of polyethylene glycol 6000, 30g of polyvinylpyrrolidone, 9g of sodium chloride, 1g of surfactant S9, EDTA-Na 2 10g, 5g of sodium benzoate, add water to 950mL, adjust the pH of the solution to 7.6, and dilute to 1L, which is the reagent R1.

[0051] The configuration method of reagent R2 is: weigh 2.42g of Tris base, 100ml of goat anti-human immunoglobulin G polyclonal antibody, 10ml of horse serum, 5g of sodium benzoate, 5g of trehalose, EDTA-Na 2 10g, Tween 20 0.5ml, add water to 950mL, adjust the pH of the solution to 7.6, and dilute to 1L, which is the reagent R2.

[0052] The preparation method of immunoglobulin G antigen diluent is: take 10╳PBS solution 200mL, horse serum 50mL, sodium benzoate 5g, Tween 1ml, add water to 950mL, adjust the pH of the solution to 7.2, and finally dilute to 1L.

[00...

Embodiment 2

[0085] A kit for detecting immunoglobulin G in urine, comprising reagent R1 and reagent R2;

[0086] The configuration method of reagent R1 is: weigh 6.057g of Tris base, 50g of polyethylene glycol 4000, 40g of polyvinylpyrrolidone, 9g of sodium chloride, 2g of surfactant S9, EDTA-Na 2 20g, 10g of sodium benzoate, add water to 950mL, adjust the pH of the solution to 7.2, and set the volume to 1L, which is the reagent R1.

[0087] The configuration method of reagent R2 is: weigh 6.057g of Tris base, 200ml of goat anti-human immunoglobulin G polyclonal antibody, 50ml of bovine serum, 10g of sodium benzoate, 3g of trehalose, EDTA-Na 2 20g, Tween 80 1ml, add water to 950mL, adjust the pH of the solution to 7.2, and dilute to 1L, which is the reagent R2.

[0088] The preparation method of immunoglobulin G antigen diluent is as follows: take 10╳PBS solution 200mL, bovine serum 10mL, sodium benzoate 10g, Tween 0.5ml, add water to 950mL, adjust the pH of the solution to 7.2, and fi...

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Abstract

The application discloses a kit for detecting immunoglobulin G in urine, which belongs to the technical field of medical in vitro diagnosis. The kit includes reagent R1 and reagent R2; the reagent R1 includes the following components: Tris base, polyethylene glycol, polyvinylpyrrolidone, sodium chloride, surfactant S9, EDTA-Na 2 and sodium benzoate; the reagent R2 includes the following components: tris base, goat anti-human immunoglobulin G polyclonal antibody, animal serum, sodium benzoate, trehalose, EDTA-Na 2 and Twain. The kit of this application has high sensitivity, wide linear range and good repeatability, and can accurately detect low-value samples; at the same time, the kit of this application also has good stability, and the reagents can be stably stored in 14 samples It eliminates the filtration step before the reagent is used, which simplifies the operation steps and improves the accuracy of the test results.

Description

technical field [0001] The application relates to the technical field of medical in vitro diagnosis, in particular to a kit for detecting immunoglobulin G in urine. Background technique [0002] Immunoglobulin (Ig) refers to a globulin having antibody (Ab) activity or a chemical structure similar to an antibody molecule. Immunoglobulin G is the main immunoglobulin in the blood, most of which exist in the form of monomers, mainly composed of spleen and lymph, and cannot be filtered by glomeruli, so the content in normal human urine is extremely low. [0003] The content of IgG in the urine of patients with primary glomerulonephritis and chronic nephritis is high, and only slightly increased in other types of glomerular diseases; IgM only appears in the urine of patients with chronic nephritis, while in primary glomerulonephritis and occult nephritis patients with very little urine content. Therefore, the type of elevated Ig in urine can be analyzed to help differentiate the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/564G01N33/53
CPCG01N33/6854G01N33/564G01N33/5306G01N2800/347
Inventor 王艳新吴鸣月张望常缘荣
Owner BEIJING DIAGREAT BIOTECH CO LTD
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