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Method for trapping and culturing AM fungi in extremely cold grassland areas

An extremely cold and fungal technology, which is applied in the field of AM fungi trapping and culture in the arctic grassland area, can solve the problems of loss of structural characteristics, loss of occasional and rare species, mistakes, etc., to ensure vitality and quantity, reduce cross-infection, Avoid the effects of infection

Pending Publication Date: 2021-07-16
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The extremely cold grassland area is mainly distributed in the central and eastern grasslands of Inner Mongolia, and has extremely rich grassland bacteria resources. However, the soil spores in the samples collected directly from the field usually have low vitality; due to the root structure, chemical composition, soil physical and chemical properties, Due to the influence of temperature, humidity and soil microbial activities, its original structural characteristics are lost or changed; in addition, the amount of plant roots and soil samples collected is small, and it is easy to lose occasional and rare species, resulting in resource omission and identification errors
Even if the abundant AM fungi are effectively collected from the soil, the richness of the strains, the vitality of the spores and the sporulation ability of the strains cannot be guaranteed in the later culture
And how to overcome the problem that the spore viability of the strain and the spore production ability of the strain after the strain is collected has become a technical problem to be solved urgently by those skilled in the art.

Method used

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  • Method for trapping and culturing AM fungi in extremely cold grassland areas
  • Method for trapping and culturing AM fungi in extremely cold grassland areas
  • Method for trapping and culturing AM fungi in extremely cold grassland areas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Collection and processing of plant roots and rhizosphere soil samples: from mid-August to mid-September when the host stops growing, the cluster mycorrhizae are fully developed, and most of the spores are mature, the target plant Stipa purpurea Dig up the whole plant of Stipa , Stipa stylus and Stipa chrysanthemum together with the root balls and put them into a large sealed bag, then remove the above-ground part, chop up the root balls and mix with the topsoil.

[0033] (2) Configuration and treatment of culture substrate: wash the sand and vermiculite with water, sterilize at 121°C, 0.1MPa high-temperature and high-pressure steam for 2 hours, and then cool it for use. Mix the sand, raw soil, and vermiculite in a volume ratio of 1:1: 1 were mixed to obtain a culture substrate.

[0034] (3) Select seeds with plump grains and uniform texture, soak them overnight with 0.5% potassium permanganate solution, rinse them with tap water, then rinse them with distilled water...

Embodiment 2

[0064] (1) Collection and processing of plant roots and rhizosphere soil samples: from mid-August to mid-September when the host stops growing, the cluster mycorrhizae are fully developed, and most of the spores are mature, the target plant Stipa purpurea Dig up the whole plant of Stipa , Stipa stylus and Stipa chrysanthemum together with the root balls and put them into a large sealed bag, then remove the above-ground part, chop up the root balls and mix with the topsoil.

[0065] (2) Configuration and treatment of culture substrate: wash the sand and vermiculite with water, sterilize at 121°C, 0.1MPa high-temperature and high-pressure steam for 2 hours, and then cool it for use. Mix the sand, raw soil, and vermiculite in a volume ratio of 1:1: 1 were mixed to obtain a culture substrate.

[0066] (3) Select 5 corn, sorghum, alfalfa, and clover seeds with full grain and uniform texture, soak them in indole acetic acid with a concentration of 20 mg / L for 12 hours, then soak the...

Embodiment 3

[0071] The composition of nutrient solution: ferrous sulfate 75mg / L, potassium dihydrogen phosphate 400mg / L, calcium nitrate 0.04mg / L, zinc nitrate 0.04mg / L, glucose 2000mg / L, edetate disodium 100mg / L, EDTA iron sodium salt 15mg / L, vitamin B0.5mg / L.

[0072] Same as Example 2, the difference is that 100 mL of nutrient solution is added before watering in step (3).

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Abstract

The invention discloses a method for trapping and culturing AM fungi in extremely cold grassland areas. The method comprises the following steps that host plants are planted in a mixed culture medium consisting of collected plant root systems, rhizosphere soil samples, sand and vermiculite, and a trapping medium is harvested after trapping and culturing. According to the method for trapping and culturing the AM fungi in the extremely cold grassland areas, the activity of strain resources in the collected soil can be guaranteed, the richness of the strains can be guaranteed, the problem that the activity of the strains is remarkably reduced after the AM fungi are collected is effectively solved, and necessary conditions are provided for strain morphology and molecular identification of the AM fungi and establishment of single strain culture of local strain resources.

Description

technical field [0001] The invention relates to the field of propagation and cultivation of soil fungi, in particular to a method for trapping and cultivating AM fungi in extremely cold grassland areas. Background technique [0002] Mycorrhizae are a common plant symbiosis phenomenon in nature, which is a combination of mycorrhizal fungal hyphae in soil and vegetative roots of higher plants. Arbuscular mycorrhiza fungi (AMF) is such a type of endomycorrhizal fungi, which can infect 90% of the roots of higher plants in terrestrial ecosystems and form mutualistic symbionts. Because some AM fungi do not produce vesicles in the roots, but all form arbuscules, this type of symbiont is called arbuscular mycorrhiza (AM). Because the hyphae of AMF are thinner and denser than the root system of the host plant, AMF can absorb soil nutrients that the root system of the host plant cannot reach, and can significantly improve the nutritional status of the host plant. Since arbuscular my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/20A01G22/22A01G22/00A01G22/40A01G24/10A01G24/15A01G24/12A01G18/40A01G18/20C12N1/14C12R1/645
CPCA01G22/20A01G22/22A01G22/00A01G22/40A01G24/10A01G24/15A01G24/12A01G18/40A01G18/20C12N1/14
Inventor 马晓丹包玉英刘海静屈翰霆李京鹏廖尚沐
Owner INNER MONGOLIA UNIVERSITY
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