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Composition and adiponectin detection kit

A composition and adiponectin technology, applied in the field of medical detection, can solve the problems of expensive chemiluminescence method, limited sensitivity, complicated operation steps of enzyme-linked immunosorbent method, etc., and achieve the effects of improving low-concentration reaction signal value and improving sensitivity

Inactive Publication Date: 2021-06-29
SHENZHEN AMTECH BIOENGINEERING LTD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme-linked immunoassay has cumbersome operation steps, long detection time, limited sensitivity and linear range, and cannot be tested in batches; chemiluminescence requires expensive equipment and takes a long time to detect; as an early predictor of type 2 diabetes, latex immunoassay The turbidimetric method is equipped with a fully automatic biochemical analyzer or a specific protein analyzer, which can achieve high-throughput and rapid testing, while taking into account the advantages of high precision and sensitivity, and has great market prospects
However, commercially available adiponectin turbidimetric kits have limited sensitivity and cannot be used universally with specific protein analyzers

Method used

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  • Composition and adiponectin detection kit
  • Composition and adiponectin detection kit
  • Composition and adiponectin detection kit

Examples

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preparation example Construction

[0107] In one embodiment, the preparation method of the ADPN antibody microsphere conjugate comprises: washing the carboxypolystyrene microspheres with 10-100 mM MES pH6.0 buffer solution for 2-3 times, and then distilling the volume to 0.5 with the above buffer solution. -2% (w / v), add carbodiimide and N-hydroxysulfosuccinimide to it, stir at room temperature for 15-40 minutes for activation, wash with 10-100mM MES or HEPES buffer for 2-3 times , set the volume to 0.5-2% (w / v), add the microsphere suspension after activation and cleaning to an equal volume of 0.01-0.2% ADPN antibody solution, stir at room temperature for 2-4 hours, add blocking agent, and continue to Stir for 2-4 hours, wash 2-3 times with 10-100mM MES or HEPES buffer, and finally disperse in a suitable storage solution to prepare ADPN antibody microsphere conjugates.

[0108] In order to solve the problems existing in the prior art, in one embodiment, the present invention provides a highly sensitive adipone...

Embodiment 1

[0117] This example provides the preparation of a blocking agent.

[0118] The formula of the sealing agent of the present embodiment is as follows:

[0119] Table 1

[0120] components Dosage per liter Glycine 75g (about 1mol) ethanolamine 50mL (about 0.82mol) BSA 50g

[0121] In Table 1, "amount per liter" refers to the amount of each component in the sealing agent per liter.

[0122] Add part of the purified water to the beaker on a magnetic stirrer, stir at a medium speed, put the above materials according to the above table, stir until the materials are completely dissolved, and adjust the pH value to 8.0-8.5, and dilute to 1 liter with purified water.

Embodiment 2

[0124] This example provides the preparation of a blocking agent.

[0125] The formula of the sealing agent of the present embodiment is as follows:

[0126] Table 2

[0127] components Dosage per liter tris hydroxymethyl amino methane 121g (about 1mol) ethanolamine 50mL sodium casein 20g

[0128] Add part of purified water to the beaker on a magnetic stirrer, stir at a medium speed, add the above materials according to the above table, stir until the materials are completely dissolved, adjust the pH value to 8.0-8.5, and dilute to 1L with purified water.

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Abstract

The invention discloses a composition and an adiponectin detection kit. The composition contains organic acid salt. By adding the organic acid salt into the detection reagent, the low-concentration reaction signal value is effectively increased, and the adiponectin detection sensitivity is further improved.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a composition and adiponectin detection kit. Background technique [0002] Adiponectin, also known as ADPN, Acrp30, AdipoQ, apM1 or GBP28, is an important regulator of lipid or carbohydrate metabolism secreted by adipocytes. ADPN exists in the form of low molecular weight trimer, hexamer and high molecular weight complex in the blood circulation, and the high molecular weight complex is the main active form of ADPN metabolism. Clinical studies have found that normal human blood circulation is rich in adiponectin, and its total plasma adiponectin concentration is 3-30.0 mg / L, accounting for about 0.01% of the total plasma protein. The level of adiponectin is significantly lower in patients with obesity, diabetes and coronary heart disease, which is an independent risk factor for coronary heart disease. Adiponectin is a newly discovered special protein that is specifica...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/543G01N21/82G01N21/59G01N21/49
CPCG01N33/74G01N33/54313G01N33/54393G01N21/82G01N21/59G01N21/49
Inventor 陈小茹张宁刘亚伦吴向东
Owner SHENZHEN AMTECH BIOENGINEERING LTD INC
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