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Method for preparing fulvic acid from straw

A technology of fulvic acid and straw, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that straw pulp black liquor lignin cannot be applied, and achieve the purpose of avoiding the effect of inhibition, reducing use, and removing growth inhibitory effect

Active Publication Date: 2022-04-29
SHANDONG AIFUDI BIOLOGICAL TECH
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Problems solved by technology

[0005] In order to solve the above problems and overcome the deficiencies of the prior art, the invention provides a method for preparing fulvic acid from straw, which can effectively solve the first problem: the straw contains cellulose and hemicellulose, and the method The medium bacteria mainly deal with cellulose, which leads to the problem that the lignin of straw pulp black liquor cannot be applied;

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  • Method for preparing fulvic acid from straw

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specific Embodiment approach

[0029] The method for preparing fulvic acid from straw is characterized in that it comprises:

[0030] (1) Fermentation of bacterial strains: Bacillus subtilis, Candida tropicalis, Aspergillus niger and white rot fungi were inoculated on YPD plate medium for 30°C cultivation and activation respectively;

[0031] (2) Primary seed cultivation: according to soybean meal hydrolyzate (300-400) g / L, corn flour (4-8) g / L, sucrose (1.0-1.5) g / L, NaCl 15g / L, MgSO 4 ·7H 2 O 8g / L, KH 2 PO 4 6g / L, (NH 4 ) 2 HPO 4 4.5g / L, prepare primary seed medium, natural pH value, inoculate the slant bacteria in 1000mL liquid medium and ferment at 30°C, 200rpm for 24h;

[0032] (3) Secondary seed cultivation:

[0033] According to soybean meal hydrolyzate (300-400) g / L, corn flour (1-5) g / L, sucrose (0.5-1) g / L, NaCl10g / L, MgSO 4 ·7H 2 O 4g / L, KH 2 PO 4 3g / L, (NH 4 ) 2 HPO 4 2g / L, prepare secondary seed liquid, natural pH value, inoculate the secondary seed liquid of Bacillus subtilis,...

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Abstract

The invention discloses a method for preparing fulvic acid from straw, which belongs to the technical field of biological fermentation, comprising successively inoculating fermented seed liquid I and fermented seed liquid II into a fermented liquid tank, and adding auxiliary material II in the second stage of fermentation, The auxiliary material II includes coenzyme Q, dextranase and chitosan; the beneficial effect of the present invention is: after softening the straw by using the alkaline environment of the straw pulp black liquor, the use of calcium oxide is reduced, and the fermented The method realizes the step-by-step degradation of cellulose and lignin in straw and pulping black liquor. Candida tropicalis can effectively inhibit the growth of white rot fungi and promote the degradation of cellulose in the early stage of fermentation. Glucanase is effective for Candida tropicalis The treatment of yeast cell walls relieves the inhibition of Candida tropicalis on the growth of white rot fungi. Chitosan can effectively inhibit the enzyme activity of cellulase and hemicellulose, avoiding the excessive degradation of cellulose while decomposing lignin in the later stage of fermentation. degradation, resulting in a low conversion rate of fulvic acid.

Description

Technical field: [0001] The invention belongs to the technical field of biological fermentation, and more specifically relates to a method for preparing fulvic acid from straw. Background technique: [0002] Alkaline pulping is a method of treating plant fiber raw materials with an aqueous solution of alkaline chemicals to dissolve the lignin in the raw materials and separate the fibers from them into pulp. After alkaline pulping, about 50% of the fiber raw materials and most of the alkaline substances are dissolved in the cooking liquor to become black liquor. The black liquor wastewater contains pentosan, lignin, sulfate soap and alkali substances. So the waste water is black. Due to the difficulty of decolorization and the serious consumption of dissolved oxygen when discharged into water, it is difficult to deal with by general methods. [0003] At present, the conversion of pulping black liquor into humic acid generally includes two types. One is to add pulping black ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P1/02C12P1/04C12R1/125C12R1/74C12R1/685C12R1/645
CPCC12P39/00C12P1/02C12P1/04
Inventor 魏祥圣仲伟莲李鹏
Owner SHANDONG AIFUDI BIOLOGICAL TECH
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