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Activated partial thromboplastin time detection reagent and kit

A technology for thromboplastin time and detection reagents, which is applied in the field of detection and can solve problems such as instability of ellagic acid and poor stability of detection reagents for thromboplastin time

Pending Publication Date: 2021-06-25
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Activated partial thromboplastin time testing (APTT) reagents usually use ellagic acid as an activator. Due to the instability of ellagic acid in liquid, the stability of activated partial thromboplastin time testing reagents is poor

Method used

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  • Activated partial thromboplastin time detection reagent and kit
  • Activated partial thromboplastin time detection reagent and kit
  • Activated partial thromboplastin time detection reagent and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Weigh 10.9g Tris into 800mL ultrapure water, keep stirring, adjust the pH value to 7.20 with HCl, and dilute the buffer solution to 1000mL to obtain Tris-HCl buffer solution.

[0044] 0.0302 g of ellagic acid was weighed and dissolved in 1 mL of dimethyl sulfoxide, and then the solution was added to the buffer solution so that the final concentration of ellagic acid was 0.1 mM to obtain a mother solution of ellagic acid.

[0045] Take 10mL of ellagic acid mother liquor, add 0.005g of rabbit cephalin, 0.05g of tryptone (OXOID.LTD, BASINGSTOKE, HAMPSHIRE, ENGLAND.LOT: 1938556), 0.002g of ascorbic acid and 0.02mL of Proclin300, stir well to obtain Ellagic acid APTT reagent.

Embodiment 2

[0047] Take 10mL of the ellagic acid solution prepared in Example 1, add 0.001g of rabbit cephalin, 0.01g of meat peptone (Yuanye Biology, LOT: A11A9B58441), 0.002g of ascorbic acid and 0.02mL of Proclin300, stir well to obtain ellagic acid Flower acid APTT reagent.

Embodiment 3

[0049] Take 10mL of the ellagic acid solution prepared in Example 1, add 0.01g of rabbit cephalin, 0.1g of tryptone (Yuanye Biology, LOT: A11A9B58441), 0.002g of ascorbic acid and 0.02mL of Proclin300, stir well to obtain tanned Flower acid APTT reagent.

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Abstract

The invention relates to an activated partial thromboplastin time detection reagent and an activated partial thromboplastin time detection kit comprising the activated partial thromboplastin time detection reagent. The activated partial thromboplastin time detection reagent comprises phospholipid, a stabilizer, an activator and a buffer solution, and the stabilizer comprises peptone. According to the activated partial thromboplastin time detection reagent, peptone is added as a stabilizer, and the peptone contains more than 20 amino acids, so that the stability of an activating agent in liquid can be improved. Compared with a traditional APTT reagent, the activated partial thromboplastin time detection reagent is better in stability.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to an activated partial thromboplastin time detection reagent and a kit comprising the activated partial thromboplastin time detection. Background technique [0002] When the vessel wall is damaged, the subendothelial tissue is exposed, and the negatively charged subendothelial collagen fibers come into contact with coagulation factors, and factor XII binds to it, and is activated with the participation of kallikrein PK and high molecular weight kallikrein HK to XIIa, which in turn activates XI to XIa, which activates IX to IXa. In the presence of calcium ions and phospholipids, IXa, VIIIa, calcium ions and phospholipids form a complex to activate prothrombin to form thrombin, and then activate fibrinogen to fibrin to coagulate plasma. [0003] The activated partial thromboplastin time is determined by coagulation method. At 37°C, specific negatively charged activators and a stan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/56
CPCC12Q1/56
Inventor 李云霞何圣侠
Owner SHENZHEN DYMIND BIOTECH
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