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Model and method for predicting yield of chitosan prepared from armadillidiam vulgare at different water bath temperatures

A technology of water bath temperature and prediction method, which is applied in the direction of instruments, biological systems, bioinformatics, etc., can solve the problems of resource waste, retention, and difficulty in industrial utilization, and achieve the effect of improving production efficiency and reducing preparatory work

Active Publication Date: 2021-06-18
BINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scholars have extracted chitin from the body wall of insects, such as the white star beetle, oil hyacinth, dung beetle, monochamus, masson pine caterpillar, etc., but these insects have relatively few sources and insufficient resources, and are still In the stage of laboratory research, there are difficulties in industrial application
[0005] Rats, also known as woodworms and watermelon worms, exist in large numbers in the Yellow River Delta region. They have large biomass, rich resources, and are easy to collect and raise. Rodent worms are used as medicine for pain relief, but the lack of large-scale utilization leads to a serious waste of resources
It is a new way to expand the resource utilization of scorpion insects for chitosan production, but at this stage, there is no prediction model for the yield of chitosan products derived from scorpions, and it must be verified by many experiments before production. Finding the best water bath temperature is time-consuming and labor-intensive

Method used

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  • Model and method for predicting yield of chitosan prepared from armadillidiam vulgare at different water bath temperatures
  • Model and method for predicting yield of chitosan prepared from armadillidiam vulgare at different water bath temperatures
  • Model and method for predicting yield of chitosan prepared from armadillidiam vulgare at different water bath temperatures

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A predictive model for the yield of chitosan prepared by chrysalis worms at different water bath temperatures. The corresponding products are demineralized extract, chitin, decolorized extract and chitosan;

[0033] The yield prediction model includes the prediction of extract yield after demineralization, the prediction of chitin yield, the prediction of extract yield after decolorization, the prediction of chitosan yield and the prediction of total yield, wherein,

[0034] Extraction yield P after demineralization 1 =0.0000243929T 1 2 -0.0053407857T 1 +0.6353100000,

[0035] Chitin yield P 2 =-0.0000174286T 2 2 -0.0017682857T 2 +0.4803742857,

[0036] Extract yield P after decolorization 3=0.0000234643T 3 2 -0.0036526429T 3 +1.0686414286,

[0037] Chitosan yield P 4 =-0.0000555000T 4 2 +0.0047692857T 4 +0.6629285714,

[0038] Total yield P t =P 1 *P 2 *P 3 *P 4 ,

[0039] In the formula, T 1 Water bath temperature for demineralization of raw ...

Embodiment 2

[0040] Example 2 Construction of a predictive model for preparing chitosan yields by worms under different water bath temperatures

[0041] (1) Construction of predictive model for extract yield after demineralization

[0042] After cleaning, drying and pulverizing the worms, the worm powder was dispersed in 10% HCl solution at a solid-to-liquid ratio of 1:10, and heated in a water bath at different temperatures for 1.5 hours. Cool to room temperature and centrifuge at 1500r / min, wash the centrifugal precipitate until the pH value of the washing liquid is neutral, then dry the centrifugal precipitate at 60°C to obtain the demineralized extract, and weigh it;

[0043] Among them, a total of 6 temperature gradients were set for the water bath heating treatment, which were 40°C, 50°C, 60°C, 70°C, 80°C, and 90°C, and each temperature gradient was repeated three times.

[0044] According to the formula: extract yield after demineralization = extract mass after demineralization / mas...

Embodiment 3

[0058] Use the yield prediction model of Example 1 to predict the product yield and total yield of each stage of chitosan prepared by chrysalis under different water bath temperatures. and deacetylation treatment in four stages, and the corresponding products of the four stages are demineralized extract, chitin, decolorized extract and chitosan, among which,

[0059] The demineralization treatment is as follows: after washing, drying and crushing the worms, the worms powder is obtained, and the worms powder is dispersed in a 10% HCl solution at a solid-to-liquid ratio of 1:10, and placed at 40-100°C Heat treatment in water bath for 1.5h;

[0060] The specific treatment of organic matter removal is: disperse the demineralized extract in 6% NaOH solution at a solid-to-liquid ratio of 1:10, and heat it in a water bath at 40-100°C for 10 hours;

[0061] The decolorization treatment is as follows: disperse chitin in 10% H at a solid-to-liquid ratio of 1:10 2 o 2 In the solution,...

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Abstract

The invention relates to the technical field of biological resource processing, in particular to a model and method for predicting the yield of chitosan prepared from armadillidiam vulgare at different water bath temperatures, and the yield prediction model comprises prediction of the yield of an extract obtained after demineralization, prediction of the yield of chitin, prediction of the yield of an extract obtained after decoloration, prediction of the yield of chitosan and prediction of the total yield. According to the method, the yield prediction model is used for predicting the product yield and the total yield of the armadillidiam vulgare in each stage in the process of preparing the chitosan from the armadillidiam vulgare. The model clearly reflects the relationship between the water bath temperature and the extraction effect of the chitosan product in different preparation stages, the method is convenient for technicians to select parameters and estimate the yield according to the production purpose, guidance is provided for the development and utilization of the extraction of the chitosan from the armadillidiam vulgare, and the resource utilization value of the armadillidiam vulgare is expanded.

Description

technical field [0001] The invention relates to the technical field of biological resource processing, in particular to a model and method for predicting the yield of chitosan prepared by musculoskeletal moths at different water bath temperatures. Background technique [0002] Chitin, also known as chitin and chitin, has a chemical name of (1,4)-2-acetylamino-2-deoxy-β-D-glucan and is a biopolymer. Chitosan, also known as polyglucosamine and deacetylated chitin, has a chemical name of (1,4)-2-amino-2-deoxy-β-D-glucan, which is the product of deacetylated chitin. The most basic and important derivative of chitin, it is generally believed that chitin with a deacetylation degree greater than 50% is chitosan. [0003] Chitin is non-toxic, degradable, has excellent film-forming properties and tissue compatibility, and has high application value in food, chemical, medical and other fields. However, chitin has poor hydrophobicity and is insoluble in water. It is only soluble in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B45/00G16B5/00C08B37/08C08B37/00
CPCG16B5/00G16B45/00C08B37/003C08B37/0003
Inventor 梅增霞李建庆杨洁
Owner BINZHOU UNIV
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