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Passiflora edulis pericarp acidic polysaccharide as well as preparation method and application thereof

A technology of acidic polysaccharide and passionflower, which is applied in the direction of pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problem of less research on biological functions in extraction and preparation, achieve less impurity content, save intermediate Steps, the effect of reducing the extraction time

Pending Publication Date: 2021-06-15
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Passiflora peel is a biological material with abundant reserves but discarded by the food processing industry. Although there have been researches on the activity of polysaccharides in it, the extraction and preparation of acidic polysaccharides, the main active component, and their biological functions less

Method used

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  • Passiflora edulis pericarp acidic polysaccharide as well as preparation method and application thereof
  • Passiflora edulis pericarp acidic polysaccharide as well as preparation method and application thereof
  • Passiflora edulis pericarp acidic polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Crush and sieve 100 g of dried passion fruit peel, condense and reflux extract with 80% ethanol at 85°C for 3 times, each time for 2 hours, collect the residue and dry it; mix the residue and water according to the ratio of material to liquid: 1g: 16mL , soak overnight, extract twice at 90°C, 4 hours each time, combine the extracts, concentrate the extracts to viscous, cool, add three times the volume of 95% ethanol; filter the extracts after alcohol precipitation, Centrifuge for 15min, get the precipitate and freeze-dry to obtain the crude polysaccharide of passion fruit peel;

[0047](2) 2g Passiflora pericarp crude polysaccharide is dissolved in 200mdL water, is loaded on CL-type DEAE-cellulose ion-exchange chromatography column, with the flow rate of 15mL / min first elutes with distilled water, discards eluent, Then use concentration to be 0.7mol / L NaCl aqueous solution elution, collect eluent concentration, running water dialysis 48h, freeze-drying, obtain passi...

Embodiment 2

[0050] (1) Crush and sieve 100 g of dried passion fruit peel, condense and reflux extract with 80% ethanol at 85°C for 3 times, each time for 2 hours, collect the residue and dry it; mix the residue and water according to the ratio of material to liquid: 1g: 16mL , soak overnight, extract 2 times at 100°C, 3 hours each time, combine the extracts, concentrate the extracts to viscous, cool, add three times the volume of 95% ethanol; Centrifuge for 15min, get the precipitate and freeze-dry to obtain the crude polysaccharide of passion fruit peel;

[0051] (2) 2g passion fruit peel crude polysaccharide is dissolved in water, loads sample on CL-type DEAE-cellulose ion-exchange chromatography column, with the flow rate of 15mL / min first elutes with distilled water, discards eluent, and then Sequentially eluted with a NaCl aqueous solution with a concentration of 0.7mol / L, collected the eluate and concentrated it, dialyzed in running water for 48 hours, and freeze-dried to obtain 0.2...

Embodiment 3

[0054] (1) Crush and sieve 100 g of dried passion fruit peel, condense and reflux extract with 80% ethanol at 85°C for 3 times, each time for 2 hours, collect the residue and dry it; mix the residue and water according to the ratio of material to liquid: 1g: 16mL , soak overnight, extract 2 times at 100°C, 3 hours each time, combine the extracts, concentrate the extracts to viscous, cool, add three times the volume of 95% ethanol; Centrifuge for 10min, get the precipitate and freeze-dry to obtain the crude polysaccharide of passion fruit peel;

[0055] (2) 2g passion fruit peel crude polysaccharide is dissolved in water, loads sample on CL-type DEAE-cellulose ion-exchange chromatography column, with the flow rate of 15mL / min first elutes with distilled water, discards eluent, and then Sequentially eluted with a NaCl aqueous solution with a concentration of 0.7mol / L, collected the eluate and concentrated it, dialyzed it in running water for 48 hours, and freeze-dried to obtain ...

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Abstract

The invention discloses passion fruit peel acidic polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of plant extraction and medicines. The preparation method of the passion fruit peel acidic polysaccharide comprises the following steps: crushing passion fruit peel, adding ethanol, performing condensing, performing refluxing and extracting, taking filter residues, performing drying, adding water, performing soaking and extracting, combining extracting solutions, performing concentrating and cooling, adding 95% ethanol, carrying out alcohol precipitation treatment, taking precipitate, and performing centrifuging to obtain crude passion fruit peel polysaccharide; carrying out DEAE cellulose ion exchange column chromatography, performing eluting with distilled water and a NaCl solution in sequence, and performing separating and purifying to obtain the passion fruit peel acidic polysaccharide. The passion fruit peel acidic polysaccharide prepared by the method has hypoglycemic activity, and can be used as a medicine for treating diabetes mellitus.

Description

technical field [0001] The invention relates to an acidic polysaccharide from passion fruit peel, a preparation method and an application thereof, and belongs to the field of plant extraction and the field of medical technology. Background technique [0002] Polysaccharides are sugars that exist in abundant resources in nature, and widely exist in organisms such as plants, animals and microorganisms. As a natural polymer extract with almost no toxic side effects, polysaccharides are used in the prevention and treatment of many diseases, especially some stubborn and difficult diseases, such as tumors, low immunity, high blood sugar, aging, viral diseases, etc. , has its own unique effects. [0003] Passion fruit, also known as passion fruit, is a subtropical special fruit. Its pulp has high acidity, strong flavor and rich nutrition. It is an excellent raw material for subtropical fruit juice processing. However, most of the passionflower processing enterprises discard the p...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCC08B37/00C08B37/0003A61K31/715A61P3/10
Inventor 孙永进李霞彭琴关媛李丽芬
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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