Method for rapid in-situ detection of triphenylmethane chemicals in living biological tissue

A technology for in-situ detection of living organisms, applied in the field of analytical chemistry, can solve the problems of various laboratory tests, high requirements for instruments and equipment, and unsuitable rapid detection, etc. , fast and efficient enrichment effect

Pending Publication Date: 2021-06-11
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Triphenylmethane chemicals mainly include malachite green, cryptomalachite green, and crystal violet; at present, the detection of triphenylmethane chemical residues is mainly performed by gas chromatography and high performance liquid chromatography. The detection of substances is usually extracted, enriched or concentrated, and then detected by electron capture, hydrogen flame, ultraviolet or other detectors after chromatographic separation. This kind of detector detection method is accurate, high precision, low detection limit, but relatively In terms of long detection time, complicated pre-treatment, high requirements for instruments and equipment, and difficult operation, it can only be used in various laboratory tests and is not suitable for rapid on-site detection.
The advantage of liquid chromatography is high accuracy, but the sample pretreatment is more complicated and time-consuming in liquid chromatography determination
[0007] Due to the complex in vivo environment, it is necessary to quickly and efficiently enrich the analytes with less damage to living organisms. Therefore, the current detection methods are only suitable for in vivo detection in water bodies or non-living organisms. Detection remains a challenge

Method used

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  • Method for rapid in-situ detection of triphenylmethane chemicals in living biological tissue
  • Method for rapid in-situ detection of triphenylmethane chemicals in living biological tissue
  • Method for rapid in-situ detection of triphenylmethane chemicals in living biological tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation of gold-silver bifunctional substrate:

[0052] a. Preparation of porous silver extraction wire: cut a silver wire with a diameter of 0.15 mm to 6 cm long, wash it with acetone, ethanol and ultrapure water respectively, use the cleaned silver wire as a working electrode, and a silver / silver chloride electrode as a reference Electrode, the platinum electrode is the counter electrode to form a three-electrode system, placed in a 0.1mol / L hydrochloric acid solution prepared by ultrapure water and concentrated hydrochloric acid, using cyclic voltammetry, the voltage range is -0.2V-0.2V, and the sweep rate is 10mV / s , circulated 15 times, and washed the prepared porous silver wire with ultrapure water for 3 times.

[0053] b. Preparation of gold-silver bifunctional substrate: containing 0.1mol / L KNO 3 with 1mmol / L HAuCl 4 The mixed solution is used as the electrolyte, the porous silver extraction wire is used as the working electrode, the silver / silver chloride...

Embodiment 2

[0056] A method for rapid in-situ detection of triphenylmethane chemicals in living biological tissues, comprising the following steps:

[0057] 1) Insert the gold-silver dual-function base into the hollow cavity of the stainless steel hollow tube needle, firstly insert the stainless steel hollow tube needle with the base into the fish body with the tip of the stainless steel hollow tube needle, and then put the hollow tube jacket on the Then retreat, so that the gold-silver dual-functional substrate in the tube is exposed to the fish meat, and the triphenylmethane chemicals in the fish meat are extracted for 10 minutes;

[0058] 2) Take out the stainless steel hollow tube needle with the substrate, and use a portable Raman spectrometer to detect, the laser wavelength is 785nm, the laser intensity is 150mW, and the integration time is 1s to obtain the Raman spectrum.

experiment example 1

[0060] 1) Prepare malachite green standard solution with different concentrations, immerse the gold-silver dual-functional substrate in the malachite green standard solution for solid-phase microextraction, and then conduct Raman detection, parallel 3 times. With the concentration of malachite green in the malachite green standard solution gradually increasing, the Raman spectrum in the 1174cm -1 The peak intensity at the peak gradually increases accordingly. Select the intensity at the peak and the combined linear curve to realize the quantitative detection of malachite green. The linear relationship between the concentration of the malachite green standard and the intensity of the characteristic peak is as follows: image 3 shown.

[0061] 2) Prepare different concentrations of leuco-malachite green standard solution, immerse the gold-silver dual-functional substrate in the leuco-malachite green standard solution for solid-phase microextraction, and then conduct Raman detect...

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Abstract

The invention relates to a method for rapid in-situ detection of triphenylmethane chemicals in living biological tissue. The method comprises the following steps: inserting a gold-silver bifunctional substrate into a hollow cavity of a stainless steel hollow tube needle, inserting the stainless steel hollow tube needle with the substrate into the living biological tissue, and extracting the triphenylmethane chemicals in the living biological tissue; and taking out the stainless steel hollow tube needle with the substrate, performing excitation irradiation on the gold-silver bifunctional substrate by using a Raman spectrometer, performing Raman spectrum detection, and analyzing and detecting according to a Raman characteristic peak. According to the detection method, the triphenylmethane chemicals in the biological tissue can be rapidly and efficiently enriched, online detection is carried out, pretreatment on living tissue is not needed, blood collection is not needed, extraction and detection are integrated, complex sample pretreatment is not needed, and the method is suitable for on-site rapid sampling detection.

Description

Technical field: [0001] The invention relates to a method for rapid in-situ detection of triphenylmethane chemicals in living biological tissues, belonging to the technical field of analytical chemistry. Background technique: [0002] With the rapid development of the national economy and the continuous improvement of the living standards of urban and rural residents, people's dietary structure is undergoing profound changes. With its low fat, low cholesterol, high protein, rich nutrition, delicious taste and other advantages, aquatic products are becoming one of the foods favored by consumers. [0003] Compared with other meat products, microorganisms are more likely to multiply in aquatic products. We can easily find various pathogenic bacteria, viruses and parasites in the intestines, skin, and muscles of aquatic products. Farmers want to prevent and control aquatic products. Animals are sick or polluted by microorganisms. Insecticides, fungicides, etc. are used in the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 张晓丽吕涵迪占金华
Owner SHANDONG UNIV
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