Preparation method of anti-aging cell telomerase active agent

A technology of telomerase and active agent, which is applied in the field of extraction, purification and preparation of astragalus total saponins, can solve the problems of body aging, inability to maintain human gene stability, human cell aging, etc., and achieve improved utilization rate, shortened extraction time, The effect of process simplification

Pending Publication Date: 2021-06-11
上海劲锦新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Telomeres that are too short cannot maintain the stability of human genes and eventually lead to aging of human cells and aging of the body

Method used

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  • Preparation method of anti-aging cell telomerase active agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Instruments and Reagents

[0031] 1.1 Instruments HWC-3 microwave extraction experimental equipment (Guohua Medical Equipment Company) AGILENT 1260 high performance liquid chromatography (USA AGILENT Company),

[0032] AB204-S electronic balance (METTLER TOLEDO Mettler-Toledo company), UV-2500PC spectrum detector (Japan Shimadzu company), KQ-250DE medical numerical control ultrasonic instrument (Kunshan Ultrasonic Instrument Company), V-800 rotary evaporation Instrument (BLLCHI, Switzerland) HSCS Ultrafine Pulverizer (Nara Machinery Institute, Japan), ZATA PALS Light Scattering Particle Size Distribution Potential Analyzer (BROOK HAVEN, USA), ORTHOPLAN Polarizing Microscope (Sidleitz Company),

[0033] 1.2 Reagents

[0034] Astragaloside IV reference substance was purchased from Aladdin Reagent Company, specification 20mg / bottle, purity>98%, 95% ethanol, absolute ethanol, methanol, n-butanol, vanillin, deionized water.

[0035] Preparation of the reference substanc...

Embodiment 2

[0040] Take 500g of astragalus fine powder and put it into HWC-3 type microwave extraction equipment, carry out 2 extractions with pure water extraction, the amount of water added for the first time is 10 times of that of astragalus fine powder and extract for 2 hours.

[0041] The amount of water added for the second time is 5 times that of Astragalus micropowder, extracted for 2 hours and the combined extract was concentrated to a solute ratio of 1;

[0042] The combined solution was left to stand for 24 hours, and the supernatant was recovered under reduced pressure to remove the alcohol smell. The concentrate was vacuum-dried for 24 hours to obtain the astragaloside crystal active substance.

[0043] 1. Determination of Astragalus saponins in Astragalus total saponins extract

[0044] 1.1 Experimental instruments and reagents

[0045] 1.1.1 Experimental Instruments

[0046] UV-2500PC Spectrum Detector (SHIMADZU, Japan),

[0047] AB204-S electronic balance (METTLER TOLE...

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Abstract

The invention relates to a process of a preparation method of an anti-aging cell telomerase active agent. The preparation method comprises the following steps: (1) taking 500g of astragalus membranaceus fine powder; (2) putting the astragalus membranaceus fine powder into an HWC-3 microwave extraction equipment instrument, performing microwave heating, performing extraction for three times by using an alcohol and water extraction method, controlling the heating power of the microwave extraction equipment to be 500w-1000w, controlling the temperature to be 70-75 DEG C / C, performing alcohol extraction twice, adding ethanol which is 5 times of the mass of 70-90% of the micro powder for the first time, performing reaction extraction for 20-30 minutes, adding 75-95% ethanol which is 5 times of the mass of the micro powder for the second time, and (3) concentrating the secondary ethanol extracting solution, removing ethanol, adding distilled water which is 10 times of the mass of the astragalus membranaceus micro powder for the third time, heating at 70-75 DEG C / C, reacting for 2 hours, standing for 24 hours, recovering the concentrate from the supernate, and carrying out vacuum drying for 48 hours to obtain an astragaloside active matter crystal. The extraction process method shortens the reaction extraction time, the extraction process is energy-saving and efficient, and the utilization rate of effective components is high.

Description

technical field [0001] The present invention relates to extraction and purification of astragalus total saponins and its preparation method Background technique [0002] The real cause of body aging is the presence of telomeres {1-2} at the ends of chromosomes in human cells. Every time a cell divides, telomeres will gradually shorten. Telomeres that are too short cannot maintain the stability of human genes and eventually lead to aging of human cells and aging of the body. The presence of telomerase can maintain and protect telomere length, and the activity of telomerase reduces telomere shortening {3}. [0003] There is a reverse transcriptase widely present in the human body, which plays a key role in activating and protecting the length of telomeres and in the process of cell growth and division {4-5}. Keelung Company (USA) is using ASTRAGLOSIDES as telomerase activators (ACTIVATORS) {6} to regulate and maintain telomere shortening to delay aging. [0004] Astragalus ...

Claims

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Application Information

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IPC IPC(8): A61K36/481A61P39/06A61K125/00
CPCA61K36/481A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P39/06
Inventor 屠承德
Owner 上海劲锦新材料科技有限公司
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