Preparation method of anti-aging cell telomerase active agent
A technology of telomerase and active agent, which is applied in the field of extraction, purification and preparation of astragalus total saponins, can solve the problems of body aging, inability to maintain human gene stability, human cell aging, etc., and achieve improved utilization rate, shortened extraction time, The effect of process simplification
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Embodiment 1
[0030] 1. Instruments and Reagents
[0031] 1.1 Instruments HWC-3 microwave extraction experimental equipment (Guohua Medical Equipment Company) AGILENT 1260 high performance liquid chromatography (USA AGILENT Company),
[0032] AB204-S electronic balance (METTLER TOLEDO Mettler-Toledo company), UV-2500PC spectrum detector (Japan Shimadzu company), KQ-250DE medical numerical control ultrasonic instrument (Kunshan Ultrasonic Instrument Company), V-800 rotary evaporation Instrument (BLLCHI, Switzerland) HSCS Ultrafine Pulverizer (Nara Machinery Institute, Japan), ZATA PALS Light Scattering Particle Size Distribution Potential Analyzer (BROOK HAVEN, USA), ORTHOPLAN Polarizing Microscope (Sidleitz Company),
[0033] 1.2 Reagents
[0034] Astragaloside IV reference substance was purchased from Aladdin Reagent Company, specification 20mg / bottle, purity>98%, 95% ethanol, absolute ethanol, methanol, n-butanol, vanillin, deionized water.
[0035] Preparation of the reference substanc...
Embodiment 2
[0040] Take 500g of astragalus fine powder and put it into HWC-3 type microwave extraction equipment, carry out 2 extractions with pure water extraction, the amount of water added for the first time is 10 times of that of astragalus fine powder and extract for 2 hours.
[0041] The amount of water added for the second time is 5 times that of Astragalus micropowder, extracted for 2 hours and the combined extract was concentrated to a solute ratio of 1;
[0042] The combined solution was left to stand for 24 hours, and the supernatant was recovered under reduced pressure to remove the alcohol smell. The concentrate was vacuum-dried for 24 hours to obtain the astragaloside crystal active substance.
[0043] 1. Determination of Astragalus saponins in Astragalus total saponins extract
[0044] 1.1 Experimental instruments and reagents
[0045] 1.1.1 Experimental Instruments
[0046] UV-2500PC Spectrum Detector (SHIMADZU, Japan),
[0047] AB204-S electronic balance (METTLER TOLE...
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