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Application of Bacillus coagulans ja845 in the preparation of drugs for preventing and/or treating Alzheimer's disease

A technology for Bacillus coagulans and Alzheimer's disease, applied in drug combination, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as inability to cure AD, reduce neuronal degeneration and improve AD pathological characteristics , Reduce the effect of Aβ plaque deposition

Active Publication Date: 2022-08-02
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current medical treatment can only delay the development of symptoms in AD patients, but cannot cure AD

Method used

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  • Application of Bacillus coagulans ja845 in the preparation of drugs for preventing and/or treating Alzheimer's disease
  • Application of Bacillus coagulans ja845 in the preparation of drugs for preventing and/or treating Alzheimer's disease
  • Application of Bacillus coagulans ja845 in the preparation of drugs for preventing and/or treating Alzheimer's disease

Examples

Experimental program
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Effect test

preparation example Construction

[0052] Preparation method of D-galactose solution: take D-galactose powder and prepare a D-galactose solution with a concentration of 12 mg / ml with physiological saline.

[0053] Aluminum chloride: purchased from Sinopharm Chemical Reagent Co., Ltd.

[0054] The preparation method of aluminum chloride solution: take aluminum chloride powder and prepare an aluminum chloride solution with a concentration of 2 mg / ml with physiological saline.

Embodiment 1

[0055] Example 1 Preparation of Bacillus coagulans JA845 bacterial suspension

[0056] 1. Strain activation: Pick Bacillus coagulans JA845 freeze-dried bacterial powder, inoculate it in LB liquid medium, and cultivate the activated strain in a shaking incubator at 50°C and 180rpm for 8-9 hours.

[0057] 2. Bacterial subculture: take the strain activation culture medium, inoculate it into a new LB liquid medium with 3% inoculum, and culture it in a shaking incubator at 50°C and 180rpm for 8-9 hours, and repeat this step to subculture the strain 3 times, repeat this step to expand the culture to obtain more bacterial liquid.

[0058] 3. Calculation of the number of viable bacteria: Dilute the bacterial solution by 10 and 10 by the method of coating plate counting. 2 , 10 3 , 10 4 , 10 5 , 10 6 , 10 7 , 10 8 and 10 9 times, the diluted bacterial liquid was evenly spread on the surface of LB solid medium, and counted after culturing for 48 h.

[0059] 4. Preparation of ba...

Embodiment 2

[0060] Example 2 The effect of Bacillus coagulans JA845 in the prevention and treatment of Alzheimer's disease

[0061] 1. Materials and methods:

[0062] 1. Establishment of Alzheimer's disease mouse model and experimental grouping

[0063] SPF grade ICR mice, 30, half male and half male, body weight 20±5 (g), maintained at constant temperature (20±2°C) and constant humidity (40-60%), maintained circadian rhythm for 12 hours, free diet, and normal rearing for one week , randomly divided into 3 groups according to body weight and gender: blank control group (n=10), AD model group (n=10), JA845 prevention group (n=10), AD model group was given sterile saline daily for 90 minutes After that, D-galactose solution (120 mg / kg) was injected intraperitoneally, and AlCl was administered by gavage. 3 Solution (20mg / kg); JA845 prevention group was given intraperitoneal injection of Bacillus coagulans JA845 bacterial suspension every day, 90 minutes later, intraperitoneal injection of ...

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Abstract

The application of Bacillus coagulans JA845 in the preparation of drugs for the prevention and / or treatment of Alzheimer's disease belongs to the field of biology. The deposit number is: CGMCCNo.19576. Bacillus coagulans JA845 was made into bacterial suspension, the number of viable bacteria ≥ 1 × 10 9 CFU / ml. In the present invention, Bacillus coagulans JA845 prevents and treats Alzheimer's disease by improving learning and memory ability, reducing neuronal cell damage, reducing Aβ deposition, and regulating intestinal flora. The invention provides a new idea for clinical prevention and treatment of Alzheimer's disease, and has broad application prospect and promotion value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of Bacillus coagulans JA845 in the preparation of medicines for preventing and / or treating Alzheimer's disease. Background technique [0002] Dementia is a syndrome characterized by a decline in memory, thinking, behavior, and ability to perform daily activities. Alzheimer's disease (AD) is a common type of senile dementia. The main pathological features of AD include β-amyloid protein (Aβ) deposition to form neuritic plaques, hyperphosphorylation of Tau protein leading to neurofibrillary tangles, loss of neurons and synapses, and activation of microglia. There are approximately 50 million AD patients in the world, with 10 million new cases each year. It is predicted that the total number of AD patients will reach 82 million by 2030 and 152 million by 2050. Alzheimer's disease can have a profound impact on patients themselves, as well as their caregiver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
CPCA61K35/742A61P25/28C12N1/20
Inventor 赵子健李盛钰杨舸赵玉娟王超高磊
Owner JILIN ACAD OF AGRI SCI
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