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A kind of probiotic freeze-dried shell composite protective agent and its application

A technology of probiotics and protective agents, applied in the direction of bacteria, microorganisms, preservation of microorganisms, etc., to achieve the effect of wide range of use, significant protective effect and high safety

Active Publication Date: 2022-06-28
BIO RACE BIOTECH HANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the malondialdehyde in the raw material composition of its selection has cytotoxicity, and has potential carcinogenicity, and use safety still needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A composite protective agent for low temperature freeze-drying of probiotics, which is composed of the following components by weight: whey protein isolate powder 1.5%, tyrosine 1.0%, arginine 1.0%, gellan gum 0.6%, Inulin 0.5%, vitamin C 0.5%, sodium alginate 0.2%, γ-polyglutamic acid 0.2%, and the balance is distilled water.

[0030] The freeze-drying process includes the following steps:

[0031] (1) controlling the temperature of the probiotic fermented liquid at 12°C, the centrifugal rotation speed of 18000 rpm, and the centrifugal time of 30 mins, removing the supernatant, preliminarily dehydrating, and collecting the probiotic bacteria;

[0032] (2) Mix the composite protective agent for freeze-drying with the dehydrated probiotic bacteria in a ratio of 3:1, put it into a vacuum freeze-drying machine for freeze-drying, drop to -33 °C at a cooling rate of 10 °C per half hour, and vacuum The temperature was maintained at 190Pa for 10 hours, and the temperature was...

Embodiment 2

[0034] A composite protective agent for low-temperature freeze-drying of probiotics, which is composed of the following components by weight: 1.1% of whey protein isolate powder, 0.5% of tyrosine, 0.7% of arginine, 0.3% of gellan gum, Inulin 0.2%, vitamin C 0.4%, sodium alginate 0.3%, γ-polyglutamic acid 0.3%, and the balance is distilled water.

[0035] The freeze-drying process includes the following steps:

[0036] (1) controlling the temperature of the probiotic fermented liquid at 15°C, the centrifugal rotation speed of 19000 rpm, and the centrifugal time of 30 mins, removing the supernatant, preliminarily dehydrating, and collecting the probiotic bacteria;

[0037] (2) Mix the composite protective agent for freeze-drying with the dehydrated probiotic bacteria in a ratio of 2.2:1, put it into a vacuum freeze-drying machine for freeze-drying, and drop it to -40°C according to a cooling rate of 10°C every half an hour, The vacuum degree was maintained at 180Pa for 10 hours...

Embodiment 3

[0039] A composite protective agent for low-temperature freeze-drying of probiotics, which is composed of the following components by weight: 1.1% of whey protein isolate powder, 0.5% of tyrosine, 0.7% of arginine, 0.3% of gellan gum, Inulin 0.2%, vitamin C 0.4%, sodium alginate 0.3%, γ-polyglutamic acid 0.2%, and the balance is distilled water.

[0040] The freeze-drying process includes the following steps:

[0041] (1) controlling the temperature of the probiotic fermented liquid at 10°C, the centrifugal rotation speed of 20000 rpm, and the centrifugal time of 20 mins, removing the supernatant, preliminarily dehydrating, and collecting the probiotic bacteria;

[0042](2) Mix the composite protective agent for freeze-drying and the dehydrated probiotic bacteria in a ratio of 3.7:1 evenly, put it into a vacuum freeze-drying machine for freeze-drying, and drop it to -30°C according to a cooling rate of 10°C every half an hour, The vacuum degree was maintained at 200Pa for 10 ...

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Abstract

The invention relates to a probiotic freeze-dried shell composite protective agent and its application. The shell composite protective agent is composed of the following components in weight percent: 0.7-1.8% of isolated whey protein powder, 0.5-1.5% of tyrosine %, arginine 0.7-1.3%, gellan gum 0.1-1.1%, inulin 0.2-1.0%, vitamin C 0.2-0.8%, sodium alginate 0.1-0.5%, γ-polyglutamic acid 0.1-0.5% , The balance is distilled water. No chemical synthetic substances are added to the shell composite protective agent of the present invention, and the safety is high; the shell composite protective agent has a very significant protective effect on probiotics before and after the freeze-drying process; Both Lactobacillus and Lactobacillus strains have a good protective effect and have broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, and in particular relates to a probiotic freeze-dried shell composite protective agent and its application. Background technique [0002] Probiotics have been widely studied and used because of the long storage and shelf life of viable bacteria and the high number of viable bacteria. The freeze-drying process generally results in changes in the cell membrane permeability of probiotics, protein denaturation and inactivation, pH damage, DNA damage, and changes in membrane fatty acid composition, etc., which lead to a large number of probiotics in the freeze-dried product. Therefore, in the production process of probiotic preparations, in order to protect the activity of probiotics, in addition to controlling the freeze-drying process, the most critical thing is to select a suitable freeze-drying protectant for probiotics as a shell protectant. [0003] Chinese patent CN 1033...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/225C12R1/23
CPCC12N1/20C12N1/04
Inventor 周定立朱智杰张勇
Owner BIO RACE BIOTECH HANGZHOU CO LTD
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