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A kind of actinomycetes with inhibitory effect on peanut soil-borne pathogenic fungus and its screening method

A technology of pathogenic fungi and inhibition, which is applied in the field of new strains, can solve the problems of environmental pollution of chemical agents, affecting the effect of prevention and control, and harming human health by drugs, so as to achieve the effects of promoting development, preventing the occurrence of key diseases, and reducing the amount of use

Active Publication Date: 2022-05-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of a certain chemical agent is likely to produce drug resistance and affect the control effect
Due to the characteristics of peanut soil-borne fungal diseases, it is necessary to apply pesticides multiple times during the peanut growth period for prevention and control. Extensive use of chemical agents will pollute the environment, cause phytotoxicity, and pose potential hazards to human health.

Method used

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  • A kind of actinomycetes with inhibitory effect on peanut soil-borne pathogenic fungus and its screening method
  • A kind of actinomycetes with inhibitory effect on peanut soil-borne pathogenic fungus and its screening method
  • A kind of actinomycetes with inhibitory effect on peanut soil-borne pathogenic fungus and its screening method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening and Identification of Bacterial Strains

[0024] The screening method of the actinomycetes that the present invention has inhibitory action to peanut soil-borne pathogenic fungus is as follows:

[0025] 1. Soil sample collection

[0026] Peanut rhizosphere soil was collected from the peanut field of Hunan Agricultural University in Furong District, Changsha City, Hunan Province, and brought back to the laboratory.

[0027] 2. Isolation of biocontrol bacteria (actinomycetes)

[0028] Separation by dilution. Put the soil sample into a bottle with glass balls and sterile water, vibrate, dilute with sterile water after standing, and prepare 10 -4 、10 -5 、10 -6 Dilution solution, draw 100 μL of the dilution solution and add dropwise to NA medium (beef extract 3g, agar 20g, sodium chloride 5g, peptone 10g, ddH 2 O 1000mL, adjust the pH to 7.2) on the plate, spread evenly, and place the culture dish upside down in a constant temperature incubator at ...

Embodiment 2

[0058] Embodiment 2: application embodiment

[0059] In May 2020, a field experiment was conducted at the Yunyuan Experimental Base of Hunan Agricultural University to control peanut soil-borne diseases with the fermentation broth of Streptomyces luteoverticillatus YY-38 strain. The peanut variety used in the test is Xianghua 2008, and the plot area is 200m 2 , 3 ridges, plant 4 rows in each ridge, the row spacing is 30cm, the hole spacing is 12cm, and 1 seed is planted in each hole. There are 3 treatments, processing 1: 25% thiamethoxam · fludioxonil · mefenaxyl seed coating agent (Hunan Xinchangshan Agricultural Development Co., Ltd.), the peanut seeds are carried out by the consumption of 350 milliliters / 100 kilograms of seeds Coating treatment, sowing after drying; treatment 2: the fermentation broth of Streptomyces luteoverticillatus (Streptomyces luteoverticillatus) YY-38 strain was diluted 10 times, soaked peanut seeds for 30min, and sowing after drying; treatment 3 (c...

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Abstract

The invention discloses a Streptomyces luteus ( Streptomyces lute overticillatus ) strain, its preservation number is CCTCC No: M2020262. The fermentation liquid of this strain has inhibitory effects on peanut spp., peanut root rot and peanut crown rot. The inhibition rates of the three pathogenic bacteria are 77.71%, 42.62% and 63.18% after the fermentation liquid is diluted 5 times. At present, there is no report in China that the same biocontrol bacteria can also treat three soil-borne fungal diseases of peanuts. The bacterium has no pathogenicity to humans and animals, can be safely used for biological control of peanut soil-borne fungal diseases, can reduce the use of chemical pesticides, reduce environmental pollution, and promote the development of green agriculture, and has a good application prospect.

Description

technical field [0001] The invention relates to a new bacterial strain capable of inhibiting peanut soil-borne pathogenic fungi and a screening method thereof. Background technique [0002] Peanut soil-borne fungal diseases include peanut blight, peanut root rot and peanut crown rot, which are one of the main diseases in peanut production. Wherein the pathogenic bacterium of peanut canker sore is Sclerotium rolfsii, the colony is round and white on the PDA medium, the mycelium is developed and grows radially, and the hyphae gather to form sclerotia in the later stage. The pathogen of peanut root rot is Fusarium spp., and the dominant pathogen is Fusarium solani (F.solani). Its small conidia are colorless, oval or kidney-shaped, and the size is 5 to 12×2-4μm, mostly unit cells; large conidia are sickle-shaped or crescent-shaped, with 3-5 septa; chlamydospores are spherical, solitary or clustered, with a diameter of 7-11μm. The pathogenic bacterium of peanut crown rot is Asp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/28A01P3/00C12R1/465
CPCC12N1/20C12N1/02A01N63/28
Inventor 易图永黎杨凯洪艳云李林
Owner HUNAN AGRICULTURAL UNIV
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