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Method for remediation of cadmium-contaminated soil by intercropping corn and wedelia chinensis and inoculating AMF

A technology of wedelium and cadmium pollution, applied in the restoration of polluted soil, etc., can solve the problems of low economic efficiency and low restoration efficiency, and achieve the effects of expanding the absorption area, improving the mineral nutrition status, and reducing the cadmium content and absorption amount of corn

Active Publication Date: 2021-06-04
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of existing cadmium-contaminated soil remediation methods with low remediation efficiency and low economic efficiency, and provide a method for intercropping corn and Wedelia and inoculating arbuscular mycorrhizal fungi to remediate cadmium-contaminated soil, which significantly Reduce the accumulation of cadmium in corn, improve the efficiency of remediation of cadmium-contaminated soil by Wedelia, achieve the purpose of remediating cadmium-contaminated soil while producing, and improve the remediation efficiency and economic efficiency at the same time

Method used

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  • Method for remediation of cadmium-contaminated soil by intercropping corn and wedelia chinensis and inoculating AMF
  • Method for remediation of cadmium-contaminated soil by intercropping corn and wedelia chinensis and inoculating AMF
  • Method for remediation of cadmium-contaminated soil by intercropping corn and wedelia chinensis and inoculating AMF

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The propagation and cultivation of embodiment 1 arbuscular mycorrhizal fungus bacterial agent

[0029] After using corn as a host plant for potted propagation, the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil is used as the surface balloon mycotic agent for inoculation. Specifically include the following steps:

[0030] S1. Fill sterilized sand (sand:soil (W:W)=1:1) culture medium to 4 / 5 of the plastic basin, and evenly inoculate Glomus terrestrial inoculum according to the inoculation amount of 4% (W:W). Spread a thin layer on the substrate, cover 2 cm of sterilized sand, water, sow 10-15 corn seeds per pot, cover 0.5 cm of sterilized sand, and move to the greenhouse for cultivation;

[0031] S2. Harvest after 5 months of potting. When harvesting, first cut off the stems and leaves of the aboveground part of the plant, place the pot in a room with relatively stable temperature and humidity to dry for two weeks,...

Embodiment 2

[0034] Example 2 Effect of inoculating AMF on the growth and absorption of cadmium in corn and Wedelia

[0035] 1. Experimental materials

[0036] (1) Seedling cultivation of the test plant Wedelia chrysanthemum

[0037]Wedelia proliferates in the form of asexual reproduction, remove its stolon leaves, cut into 8-10 cm long stem segments and ensure that each segment has two nodes, and place the internodes at the lower end of its morphology in a solution containing rooting powder After soaking for 4 to 5 hours, take out the stem strips and transfer them to a culture bottle equipped with tap water for room temperature cultivation in an incubator, wherein the culture bottle is wrapped with tinfoil for shading; After the shoots grow on the section, the seedlings are transplanted into pots filled with soil for cultivation.

[0038] (2) Maize Seedling Breeding for Test Plants

[0039] The test plant is corn, the variety is Jingke 516; before germination, the seeds are soaked in 1...

Embodiment 3

[0065] Example 3 The method of intercropping corn and Wedelia and inoculating AMF to remediate cadmium-contaminated soil

[0066] 1. Experimental material: CdCl added to soil 2 2.5H 2 O at a concentration of 1 mg Cd kg -1 . Other reference example 2.

[0067] 2. Experimental process:

[0068] The experimental treatment of each group is as follows:

[0069] Single cropping-M: single cropping of corn or wedelia without inoculation with AMF-Globus terrescens;

[0070] Intercropping-M: Intercropping of corn and Wedelia is not inoculated with AMF-Glomanus surface;

[0071] Single cropping + M: single cropping of corn or wedelia and inoculation with AMF-Globus terrescens;

[0072] Intercropping+M: Corn and Wedelia intercropped and inoculated with AMF-Glomanus terrestris.

[0073] Among them, the intercropping ratio of Wedelia and corn was 1:1, the plant spacing was 10 cm, and the row spacing was 10 cm.

[0074] The test pot is a plastic flowerpot with a capacity of 11kg of ...

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Abstract

The invention belongs to the technical field of heavy metal pollution remediation and agricultural product planting, and particularly relates to a method for remediation of cadmium-contaminated soil by intercropping corn and wedelia chinensis and inoculating AMF. According to the method, wedelia chinensis and corn are planted in cadmium-contaminated soil in an intercropping mode, and an arbuscular mycorrhizal fungus agent is added into the cadmium-contaminated soil, wherein the arbuscular mycorrhizal fungi infect roots of the wedelia chinensis and the corn and are symbiotic in a host, and a large amount of outside-root arbuscular mycorrhizal fungi hyphae extending into the soil enlarge the absorption area of the roots and improve the mineral nutrition status of the corn and the wedelia chinensis, especially P nutrition, so that the growth of the corn and the wedelia chinensis is promoted. Meanwhile, by inoculating arbuscular mycorrhizal fungi, the cadmium content and absorption amount of the corn are reduced, and the safety of agricultural products is guaranteed; on the contrary, the cadmium content and absorption amount of the wedelia chinensis are improved, and the efficiency of remediation of the cadmium-contaminated soil by the wedelia chinensis is further improved.

Description

technical field [0001] The invention belongs to the technical fields of heavy metal pollution restoration technology and agricultural product planting technology. More specifically, it relates to a method for intercropping corn and Wedelia and inoculating AMF to remediate cadmium-contaminated soil. Background technique [0002] Due to human activities such as farmland sewage irrigation, solid waste application, mining and smelting, soil cadmium pollution is becoming more and more serious. Moreover, cadmium has high mobility and high toxicity in the soil, and is one of the most toxic inorganic pollutants in the environment; when cadmium is absorbed by plants, it enters the plant body, and can enter the human body through the food chain enrichment, seriously threatening agricultural production and human health. Therefore, measures must be taken to reduce the heavy metal content in soil and crops to ensure the safety of agricultural production. [0003] Phytoremediation refer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 靖元孝雷丽莉朱琪英彭长连
Owner SOUTH CHINA NORMAL UNIVERSITY
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