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The method of intercropping corn and wedelia and inoculating amf to restore cadmium-contaminated soil

A technology of wedelium and cadmium pollution, applied in the restoration of contaminated soil, etc., can solve the problems of low repair efficiency and low economic efficiency, and achieve the effects of improving mineral nutrition, expanding absorption area, and ensuring safety

Active Publication Date: 2022-06-24
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of existing cadmium-contaminated soil remediation methods with low remediation efficiency and low economic efficiency, and provide a method for intercropping corn and Wedelia and inoculating arbuscular mycorrhizal fungi to remediate cadmium-contaminated soil, which significantly Reduce the accumulation of cadmium in corn, improve the efficiency of remediation of cadmium-contaminated soil by Wedelia, achieve the purpose of remediating cadmium-contaminated soil while producing, and improve the remediation efficiency and economic efficiency at the same time

Method used

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  • The method of intercropping corn and wedelia and inoculating amf to restore cadmium-contaminated soil
  • The method of intercropping corn and wedelia and inoculating amf to restore cadmium-contaminated soil
  • The method of intercropping corn and wedelia and inoculating amf to restore cadmium-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 The propagation culture of arbuscular mycorrhizal fungal agent

[0029] After pot propagation using maize as a host plant, a mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil was used as the inoculated surface glomus fungal agent. Specifically include the following steps:

[0030] S1. Load sterilized sandy soil (sand: soil (W:W)=1:1) medium to 4 / 5 of the plastic pot, and uniformly inoculate the surface Glomus inoculum according to the inoculum amount of 4% (W:W). Lay it on the substrate in a thin layer, cover with 2cm of sterilized sand, water, sow 10-15 corn seeds / pot, cover with 0.5cm of sterilized sand, and move to the greenhouse for cultivation;

[0031] S2. Harvest after 5 months of potted planting. When harvesting, first cut off the stems and leaves of the above-ground parts of the plant, place the pot in a room with relatively stable temperature and humidity to dry for two weeks, and then harvest ...

Embodiment 2

[0034] Example 2 Effects of inoculation with AMF on the growth and absorption of cadmium in corn and Wetflower

[0035] 1. Experimental materials

[0036] (1) Seedlings of the test plant Wedlock chrysanthemum

[0037]Wedge chrysanthemum is propagated asexually, its stolon leaves are removed, cut into 8-10 cm long stem segments and ensure that each segment has two nodes, and the internodes at the lower morphological end are placed in a solution containing rooting powder After soaking for 4 to 5 hours, the stem strips are taken out and transferred to a culture bottle with tap water for cultivation at room temperature in an incubator, wherein the culture bottle is covered with tin foil to block light; After the shoots grow on the segment, the seedlings are transplanted into pots with soil for cultivation.

[0038] (2) Test plant corn seedlings

[0039] The tested plant was corn, and the variety was Jingke 516; before germination, the seeds were soaked in 10% NaClO for 10 min t...

Embodiment 3

[0065] Embodiment 3 The method for intercropping of corn and Wee chrysanthemum and inoculating AMF to repair cadmium-contaminated soil

[0066] 1. Experimental material: CdCl added to soil 2 ·2.5H 2 O at a concentration of 1 mg Cd kg -1 . Other Reference Example 2.

[0067] 2. Experimental process:

[0068] The experimental treatment of each group is as follows:

[0069] Monocropping-M: Monocropping of maize or Wetflower and not inoculated with AMF-Glomus terrestris;

[0070] Intercropping-M: intercropping of maize and Wetflower without inoculation with AMF-Glassus terrestris;

[0071] Monocropping + M: Maize or Wedwig chrysanthemum monocropping and inoculated with AMF-Glomus terrestris;

[0072] Intercropping + M: Intercropping of maize and Wedlock and inoculated with AMF-Glomus terrestris.

[0073] Among them, the intercropping ratio of wedge chrysanthemum and corn was 1:1, the plant spacing was 10 cm, and the row spacing was 10 cm.

[0074] The test pot is a plasti...

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Abstract

The invention belongs to the technical fields of heavy metal pollution restoration technology and agricultural product planting technology, and in particular relates to a method for intercropping corn and wedelia and inoculating AMF to restore cadmium-contaminated soil. The method adopts the method of intercropping to plant wedelia and corn in the soil polluted by cadmium, and adds the arbuscular mycorrhizal fungal agent to the soil polluted by cadmium; wherein the arbuscular mycorrhizal fungus infects the roots of wedelia and corn And symbiotic with the host, a large number of extra-root arbuscular mycorrhizal mycelia extending into the soil expand the absorption area of ​​the root system, improve the mineral nutrition status of corn and wedelia, especially the P nutrition, thereby promoting the growth of corn and wedelia. At the same time, the inoculation of arbuscular mycorrhizal fungi reduced the cadmium content and absorption of corn, ensuring the safety of agricultural products; on the contrary, it increased the cadmium content and absorption of Wedelia, and further improved the efficiency of Wedelia remediation of cadmium-contaminated soil.

Description

technical field [0001] The invention belongs to the technical fields of heavy metal pollution restoration technology and agricultural product planting. More specifically, it relates to a method of intercropping corn and wedgeflower and inoculating AMF to remediate cadmium-contaminated soil. Background technique [0002] Due to human activities such as farmland sewage irrigation, solid waste application, mining and smelting, soil cadmium pollution is becoming more and more serious. Moreover, cadmium is highly mobile and highly toxic in soil, and is one of the most toxic inorganic pollutants in the environment. When cadmium is absorbed by plants and enters the plant body, it can be enriched into the human body through the food chain, which seriously threatens agricultural production and human health. Therefore, measures must be taken to reduce the content of heavy metals in soil and crops to ensure the safety of agricultural production. [0003] Phytoremediation refers to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 靖元孝雷丽莉朱琪英彭长连
Owner SOUTH CHINA NORMAL UNIVERSITY
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