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A method of using arbuscular mycorrhizal fungi to strengthen Solanum nigrum to absorb cadmium in soil

A technology of arbuscular mycorrhizal fungi and nightshade, applied in the field of soil pollution remediation, can solve the problems of no obvious increase in Zn absorption, complicated effects of strengthening effect, etc., achieving good treatment effect, low operating cost, no secondary The effect of pollution

Inactive Publication Date: 2016-04-27
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marques et al. (2007) showed that after inoculation with arbuscular mycorrhizal fungi, the concentration of Zn in Solanum solanum increased slightly, but the growth and biomass of Solanum solanum were not affected, so the Zn uptake of Solanum solanum inoculated with AMF was the same as that without There is no obvious improvement in the inoculation comparison, that is, the way of plant inoculation with AMF may not be able to achieve the enhanced absorption of all heavy metals; at the same time, the strengthening steps, methods and specific conditions interact with each other, and the impact on the final strengthening effect is complex and vital
So far, there are no research and application reports on whether AMF can enhance the ability of Solanum nigrum to absorb soil Cd

Method used

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  • A method of using arbuscular mycorrhizal fungi to strengthen Solanum nigrum to absorb cadmium in soil
  • A method of using arbuscular mycorrhizal fungi to strengthen Solanum nigrum to absorb cadmium in soil

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Effect test

Embodiment 1

[0041] Embodiment 1 prepares arbuscular mycorrhizal fungus inoculum

[0042] Arbuscular mycorrhizal fungi Glomus versiforme Provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences. Using corn ( Zeamays ) as a host plant for potted propagation, the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil is used as an inoculant.

[0043] Concrete preparation process is as follows:

[0044] S1. Mix the arbuscular mycorrhizal fungus inoculant with the sterilized sand evenly according to the inoculation amount of 3% according to the mass ratio, and then fill the mixed matrix into 2 / 3 of the aseptic basin;

[0045] The sand:soil ratio of the sandy soil is 1:1; the arbuscular mycorrhizal fungus inoculant is provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0046] S2. Sow 10-15 corn seeds / pot, cover with a lay...

Embodiment 2

[0052] Example 2 The research on arbuscular mycorrhizal fungus strengthening Solanum solanum to absorb cadmium in soil

[0053] 1. Experimental materials

[0054] (1) Test plant Solanum nigrum

[0055] The test plant Solanum nigrum ( Solanum nigrum ), sterilize the surface of the seeds with 10% NaClO for 10 minutes before sowing, wash them with deionized water, soak the washed Solanum nigrum seeds in ultra-pure water for 4-6 hours, wait until the seeds are fully swollen, and put them in a pad with filter paper Put them in a sterile petri dish at room temperature of 28°C. After the seeds germinate, select seedlings with consistent growth for later use.

[0056] (2) The soil to be tested was collected from the Biological Garden of South China Normal University. The soil was air-dried naturally, ground, passed through a 1mm sieve, and sterilized by continuous high-temperature and high-pressure steam for 2 hours (121°C), and different concentrations of CdCl were added 2 , so ...

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Abstract

The present invention provides a method of applying the application of the roots of branches (AMF) in improving the application of dragon sunflower growth and absorbing the ability of heavy metals in the soil, and a method of strengthening the dragon sunflower to absorb cadmium in the soil.The present invention uses cadmium (CD) ultra -cumulative plant dragon sunflower as the experimental plant, with AMF as a vaccinant, combining CD super -cumulative plant dragon sunflower and AMF with AMF with plant growth.The ability to improve the repair efficiency of Dragon sunflower on CD pollution soil.Compared with traditional chemistry and agricultural measures, the enhanced measures of the present invention have the characteristics of good governance effects, no secondary pollution, and low operating costs, and have good theoretical and application promotion value.

Description

technical field [0001] The invention belongs to the technical field of soil pollution restoration. More specifically, it relates to a method of using arbuscular mycorrhizal fungi to enhance Solanum nigrum uptake of cadmium in soil. Background technique [0002] Cadmium is a highly mobile and highly toxic heavy metal that accumulates, has no clear physiological function on plants, and is also one of the common pollutants that endanger human health. When soil and other environments are polluted by cadmium, cadmium can be enriched in organisms and enter the human body through the food chain to cause chronic poisoning. After cadmium is absorbed by the human body, it forms cadmium-sulfur protein in the body and selectively accumulates in the liver and kidney. Among them, the kidney can absorb nearly 1 / 3 of cadmium in the body, and is the "target organ" of cadmium poisoning. Other organs such as spleen, pancreas, thyroid and hair also have a certain amount of accumulation. Due...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12N1/14C12R1/645
Inventor 靖元孝刘桧谭石云袁鸣江秋云
Owner SOUTH CHINA NORMAL UNIVERSITY
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