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IgM antibody detection diluent

A diluent and antibody technology, which is applied in the field of immunodiagnosis, can solve the problems of many false positives and poor sensitivity, and achieve the effects of increasing sensitivity, improving false positives, and accurate and sensitive IgM antibody detection results

Pending Publication Date: 2021-06-01
GUANGDONG FAPON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a test diluent to be tested for IgM antibody chemiluminescence detection and the IgM antibody chemiluminescence detection kit using it, so as to alleviate false positives in the chemiluminescence immunodetection of IgM antibody-related items in the prior art. Problems with more cases and less sensitivity

Method used

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  • IgM antibody detection diluent
  • IgM antibody detection diluent
  • IgM antibody detection diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]A test dilution for IgM antibody chemiluminescence detection, including the following components: 0.05 mol / lph7.5 HEPES buffer, 25 mmol / L arginine, 500 mmol / l KCl, 0.1W / V% Tween 20, 1 W / V% Chapter, 2w / V% mannitol, 1 mol / L urea and 10 mmol / LEDTA.

Embodiment 2

[0050]This embodiment relates to a detection method of a giant cell IgM antibody.

[0051]1. Preparation of the kit.

[0052]SME IgM antibody chemiluminescence immunoassay assay kit: including the following steps:

[0053]1) Preparation of mouse anti-human IgM antibody:

[0054]The mouse anti-human IgM antibody was immunized by naturally extracted human IgM antibody, and B lymphocytes capable of specializing in synthesis anti-human IgM antibodies; then use cell hybridization technology, bone marrow cells and B lymphocytes in the same order First, obtain hybrid myeloma cells, carry out in vitro culture, during culture, hybridoma cells produce and secrete anti-human IgM monoclonal antibody, collect culture supernatant, purified to obtain the desired mouse anti-human IgM monoclonal antibody .

[0055]2) Magnetic bead system: magnetic beads γFE2O3Or Fe3O4The magnetic nanoparticles with the composite of the organic polymer material, with a particle size of 0.1 to 5 μm, with a carboxyl functional group ...

Embodiment 3

[0082]The detection of the giant cell IgM antibody detection reagent in Example 2 was carried out without adding a test diluent as a control group, and the dilution was tested in 50 mM hepes + 0.5 m KCl 1; in the experimental group conditions 1; 0.5 mKCl and 2W / V% mannitol buffer test dilution experimental group conditions 2; a buffer test dilution experimental group condition 3 containing 50 mM hepes was added to 0.5 m kCl, 2W / V% mannitol and 0.025m arginine. 50 mM HEPES was added to 0.5 mKCl, 2W / V% mannitol, 0.025 m arginine and 1M urea buffer test dilution experimental group conditions 4; to add test dilution in Example 1 as experimental group conditions 5, Yin properties control C0, weak male quality control C1 and yang properties control C2, 4 clinical-negative serum samples (negative samples 1-4), as shown in the following table:

[0083]

[0084]

[0085]Compare the signal value deviation of the control group and the five conditions and the signal-to-noise ratio P / N (the ratio...

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Abstract

The invention relates to the field of immunodiagnosis, and particularly provides an IgM antibody detection diluent. The to-be-detected test diluent for IgM antibody detection comprises a stabilizer, and the stabilizer comprises mannitol and / or urea. The inventor finds that the problems of high false positive and low sensitivity of the detection reagent can be remarkably improved by adding the stabilizer into the diluent to be detected and tested. The to-be-detected test diluent is added into the IgM antibody detection kit, so that the sensitivity and the accuracy of the kit can be effectively improved, more requirements are met, and the application range is expanded.

Description

Technical field[0001]The present invention relates to the field of immunization, and in particular, to an IgM antibody detection dilution.Background technique[0002]IGM is an abbreviation of Immunoglobulin M, meaning immunoglobulin M, divided into five kinds of immunoglobulins depending on the structure, and IGM is one of human immunoglobulins, and other IGA, IgG, IGD and IgE are also. IGM is the first IG of fetal synthesis. The IgM antibody accounts for 5% -10% of the total serum antibody, and the IgM antibody is one of the molecular weight in the antibody, and cannot be passed through the placenta to the fetus. IgG antibodies are the main antibody components in serum and extracellular fluid, accounting for about 75% of the total serum antibody. IgG antibodies are one of the least molecular weights in the antibody, which can be transferred to the fetus through the placenta, protecting the baby within six months from infection. The IgG antibody produces late, the maintenance time is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 叶小琴王文斌夏良雨
Owner GUANGDONG FAPON BIOTECH CO LTD
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