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Identification and adulteration inspection methods for component of fruit of Chinese magnoliavine in eyesight-improving pills with stem of noble dendrobium

An inspection method, the technology of luminous pills, is applied in the direction of material separation, analysis materials, instruments, etc., which can solve the problems of high cost and troublesome operation, and achieve the effects of low cost, easy operation, specificity and durability

Pending Publication Date: 2021-05-28
广西壮族自治区食品药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the TLC method introduced in this article needs to use the negative samples of northern Schisandra, southern Schisandra medicinal materials and Dendrobium Yeguang pills lacking Schisandra medicinal materials, the cost is high and the operation is more troublesome. Therefore, it is not suitable to establish Dendrobium Yeguang pills. TLC identification standard of Schisandra chinensis

Method used

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  • Identification and adulteration inspection methods for component of fruit of Chinese magnoliavine in eyesight-improving pills with stem of noble dendrobium
  • Identification and adulteration inspection methods for component of fruit of Chinese magnoliavine in eyesight-improving pills with stem of noble dendrobium
  • Identification and adulteration inspection methods for component of fruit of Chinese magnoliavine in eyesight-improving pills with stem of noble dendrobium

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Experimental program
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Effect test

Embodiment 1

[0037] A method for identifying the components of Schisandra chinensis in Dendrobium Yeguang Pills, which uses thin-layer chromatography to identify Schisandrin A as a reference substance. If the chromatogram of the test product shows spots of the same color at the position corresponding to the chromatogram of the reference substance , it can be judged that the test product contains Schisandra components; the details are as follows:

[0038] (1) Preparation of the test solution: take 6g of water honeyed pills and grind them; or take 9g of small or big honeyed pills and cut them into pieces; add 50mL of methanol, heat and reflux on a water bath for 1h, let cool, filter, and filtrate Evaporate to dryness, add 25mL of water to the residue to dissolve, shake and extract twice with petroleum ether with a boiling range specification of 30-60°C, 25mL each time, combine the petroleum ether liquid, evaporate to dryness, add the residue to petroleum ether with a boiling range specificati...

Embodiment 2

[0054] A method for adulteration inspection of schisandra components in Dendrobium Yeguang pills is to use high-performance liquid chromatography-mass spectrometry to detect the contents of schisandrin A and schisandrin in Dendrobium luminous pills, and then calculate the content of schisandrin and schisandrin The ratio of the first content, through the content ratio of the two to determine the authenticity of Schisandra in Dendrobium Yeguang Pills, as follows:

[0055] (1) Chromatographic conditions: Octadecylsilane bonded silica gel was used as filler, acetonitrile was used as mobile phase A, 10mmol / L ammonium acetate solution was used as mobile phase B, and gradient elution was carried out at a flow rate of 0.25mL / min; The elution program is: 0~2min, 10%A; 2~16min, 10%A→60%A; 16~17min, 60%A→90%A; 17~20min, 90%A; 20~20.5min, 90%A→10%A; 20.5~24min, 10%A.

[0056] (2) Mass spectrometry conditions: use electrospray ionization source, segmented acquisition, positive ion scannin...

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Abstract

The invention discloses identification and adulteration inspection methods for the component of the fruit of Chinese magnoliavine in eyesight-improving pills with the stem of noble dendrobium. According to the identification method, the eyesight-improving pill with the stem of noble dendrobium is identified by taking schizandrin as a reference substance through thin-layer chromatography. The adulteration inspection method comprises the following steps: detecting the contents of schizandrin and anwuligan in the eyesight-improving pill with the stem of noble dendrobium by adopting a high performance liquid chromatography-mass spectrometry, and then judging the authenticity of the stem of noble dendrobium in the eyesight-improving pill according to the ratio of the content of the anwuligan to the content of the schizandrin. By establishing a thin-layer chromatography identification method and a high performance liquid chromatography-mass spectrometry detection method for the stem of noble dendrobium in the eyesight-improving pill, the quality condition of the stem of noble dendrobium in the eyesight-improving pill is investigated, whether a production enterprise has the behavior of no feeding or adulterated feeding or not is checked, the method is simple, the cost is low, and the result is accurate. The medication safety of the eyesight-improving pill can be effectively ensured.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicine, in particular to a method for identifying and adulterating Schisandra components in Dendrobium Yeguang pills. Background technique [0002] Dendrobium luminous pills are composed of Dendrobium, ginseng, yam, Poria cocos, licorice, cistanche, medlar, dodder, rehmannia glutinosa, rehmannia glutinosa, Schisandra chinensis, Radix Asparagus, Ophiopogon japonicus, bitter almond, Fangfeng, Chuanxiong, bran fried aurantium, Coptis chinensis, Achyranthes bidentata , Chrysanthemum, Tribulus terrestris, green scorpion, cassia, buffalo horn concentrated powder, goat horn and other twenty-five flavors of traditional Chinese medicine. In addition to the concentrated buffalo horn powder, the goat horn file is ground into fine powder, and the other twenty-three medicinal materials such as dendrobium are ground into fine powder; the concentrated buffalo horn powder is mix...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/91G01N30/94G01N30/95G01N30/02G01N30/06G01N30/72
CPCG01N30/90G01N30/91G01N30/94G01N30/95G01N30/02G01N30/06G01N30/72Y02A50/30
Inventor 罗轶何颂华覃丽郦黄清泉白桂昌
Owner 广西壮族自治区食品药品检验所
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