Preparation method and application of mogroside V standard sample

A mogroside and standard sample technology, applied in the field of analytical chemistry, can solve the problems of complex purification difficulty of mogroside V analogs, inability to realize industrialized large-scale production, long delivery period, etc., and achieve stable product quality and preparation speed. Fast, high-volume results

Pending Publication Date: 2021-05-28
SECOND MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is no research institution in China that can provide mogroside V standard samples and their preparation methods, and importing mogroside V standard samples from abroad has problems such as high costs, cumbersome procedures, and long delivery cycles
On the other hand, mogroside V analogues are complex and difficult to purify, and relying on direct extraction from Luo Han Guo cannot achieve large-scale industrial production

Method used

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  • Preparation method and application of mogroside V standard sample
  • Preparation method and application of mogroside V standard sample
  • Preparation method and application of mogroside V standard sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of the test solution: dissolving the industrial-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolving it, and then filtering through a 0.22 μm filter membrane to obtain the test solution;

[0037] (2) Liquid chromatography preparation: inject the solution of the test sample into the preparation chromatography to separate pure fractions, wherein the chromatographic conditions: the chromatographic column is PREP-ODS(H) KIT (250mm×20mm, 5 μm), mobile phase: 23% (v / v) Acetonitrile aqueous solution; flow rate: 5-15mL / min; column temperature: 30°C; running time: 15min; detection wavelength: 202nm, injection volume 0.1-1mL, and collect fractions for 9-11min;

[0038] (3) The drying of the fractions is to freeze-dry in a freeze dryer at -60 to -40°C for 20 to 30 hours, and after the freeze-dried mogroside V solid is ground and pulverized, it is freeze-dried again for 20 to 30 hours to obtain Luo Han Guo Glycoside...

Embodiment 2

[0041] (1) Preparation of the test solution: dissolve the technical-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolve, then filter through a 0.45 μm filter membrane to obtain the test solution;

[0042] (2) liquid chromatographic preparation: inject the test solution into the preparation chromatographic separation pure fraction, wherein chromatographic conditions: chromatographic column is WatersXTerraRP18 (250mm * 20mm, 5 μ m), mobile phase: 25% (v / v) acetonitrile aqueous solution; Flow velocity : 10mL / min; column temperature: 25°C; running time: 15min; detection wavelength: 205nm, injection volume 0.1-1mL, collect fractions for 9-11min;

[0043] (3) The fractions were dried by placing them in a lyophilizer at -50°C for 24 hours. After the lyophilized mogroside V solids were ground and pulverized, they were lyophilized again for 24 hours to obtain mogroside V standard samples.

[0044] Store samples in a -18°C freezer.

[0045...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a preparation method and application of a mogroside V standard sample. The preparation method provided by the invention comprises the following steps: 1) dissolving an industrial-grade siraitia grosvenorii crude extract in an organic solvent, performing dissolving with ultrasonic assistance, and performing filtering with a filter membrane to obtain a test solution; (2) separating the test solution obtained in the step (1) by using a high performance liquid chromatography, and collecting fractions after systematic elution for 9-11 minutes; and 3) fully freeze-drying and drying the fraction collected in the step 2) to obtain the mogroside V standard product. The preparation method disclosed by the invention is economical, rapid and effective, and can make up the technical blank in the aspect of development of the mogroside V standard sample in China.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a preparation method and application of a mogroside V standard sample. Background technique [0002] Mogroside V (Mogroside V) is the main source of the sweetness of Luo Han Guo. It has pharmacological effects such as cough and expectorant, anti-cancer, anti-oxidation, and blood sugar regulation. Its sweetness value at a concentration of 1 / 10,000 is 5% of sucrose 425 times. Therefore, it has become a new generation of functional sweeteners developed by domestic and foreign countries, and it is also a high-quality excipient in the pharmaceutical industry. [0003] However, there are many difficulties in the cultivation of Luo Han Guo, and the content of mogroside V in the whole fruit is only 0.8%-1.3% W / W, but one of the main indicators to evaluate the quality of Luo Han Guo is the high content of mogroside V . Therefore, in order to effectively screen the Luo Ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈巧谢晓烨王小宁宋云飞李杰
Owner SECOND MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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