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Method for early diagnosis of AKI of severe patient

An early diagnosis and patient technology, applied in the field of medical diagnosis, can solve the problems of delayed elevation of serum markers, failure to respond to kidney damage in time, and achieve the effect of assisting the early identification of patients with cerebrovascular disease and kidney damage

Pending Publication Date: 2021-05-14
ZHONGNAN HOSPITAL OF WUHAN UNIV
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AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for the early diagnosis of AKI in critically ill patients, which solves the problem that the existing serum markers such as creatinine often delay the rise and cannot reflect the kidney damage in time

Method used

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  • Method for early diagnosis of AKI of severe patient
  • Method for early diagnosis of AKI of severe patient
  • Method for early diagnosis of AKI of severe patient

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Experimental program
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Embodiment

[0033] A method for early diagnosis of AKI in critically ill patients, comprising the following steps:

[0034] S1: The blood of the patients was collected on the first day after enrollment, and after venipuncture, the blood was drawn and collected into heparinized test tubes.

[0035] S2: Centrifuge the specimen at 2-8°C at 1000 rpm for 10-20min 25-35min after collection, take the supernatant and store it in a -80°C refrigerator.

[0036] S3: On the first day after enrollment, the midstream urine of the patients was collected, centrifuged at 100 rpm and stored in a -80°C refrigerator.

[0037] S4: Within 25-35 days after the specimen is collected, use the double antibody sandwich ELISA method to quantitatively measure the contents of the markers in plasma and urine.

[0038] S5: Coat each marker antibody in a 96-well microwell plate to make a solid phase carrier, and set standard wells, sample wells to be tested, and blank wells respectively.

[0039] S6: Set up 7 standard ...

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Abstract

The invention relates to the technical field of medical diagnosis, and discloses a method for early diagnosis of AKI of severe patients, which comprises the following steps: collecting patient blood on the first day after group entering, after venipuncture, extracting the blood and collecting the blood into a heparinized test tube, centrifuging a specimen collected in 25-35 minutes for 10-20 minutes at the temperature of 2-8 DEG C and at the speed of 1000 r / min, taking supernate, sub-packaging, and storing in a refrigerator at 80 DEG C below zero. According to the invention, urine markers capable of early diagnosis of acute kidney injury of cerebrovascular critical patients are found by analyzing a concentration curve of urine markers according to time change, the relationship between the markers and acute kidney injury is analyzed by multi-factor regression, and reference values of [TIMP-2]*[IGFBP-7] for early diagnosis of acute kidney injury (including different degrees) are calculated. The change of time concentration curves of [TIMP-2]*[IGFBP-7] in patients with cerebral vascular severe AKI and patients without cerebral vascular severe AKI is compared. The invention provides test paper capable of rapidly and sensitively detecting [TIMP-2]*[IGFBP-7] and a kit with high specificity.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a method for early diagnosis of AKI in critically ill patients. Background technique [0002] The incidence of severe stroke in China is increasing year by year, and it is also the most common neurological disease. The sequelae are often very serious, including acute kidney injury and chronic renal insufficiency. Stroke-related acute kidney injury has been reported many times in the past, and its incidence rate is as high as 30.18%. Among them, the proportion of people who maintain hemodialysis for end-stage renal disease also increased, and long-term hospitalization after acute kidney injury was significantly positively correlated with the incidence of long-term adverse events in patients, including changes in serum creatinine, increased risk of chronic kidney disease, and eventually developed to require Renal replacement therapy. [0003] Kidney-brain interaction al...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/58G16H50/20
CPCG01N33/6893G01N33/543G01N33/581G16H50/20G01N2800/2871G01N2800/347
Inventor 彭志勇李一鸣张婧张嘉皓刘芳
Owner ZHONGNAN HOSPITAL OF WUHAN UNIV
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