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Ligusticum wallichii polysaccharide as well as preparation method, identification method and application thereof

A technology of Ligusticum chuanxiong polysaccharide and identification method, which is applied in the directions of material separation, instruments, analysis materials, etc., can solve the problems of insufficient in-depth research on the structure of Ligusticum chuanxiong polysaccharide and little immunomodulatory effect, and achieves well-preserved components, enhanced phagocytic ability, and reaction conditions. mild effect

Active Publication Date: 2021-05-14
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on polysaccharides from Rhizoma Chuanxiong mainly focuses on the extraction process and antioxidant activity of its crude polysaccharides. The research on the polysaccharides from Rhizoma Chuanxiong is not deep enough, and there are few reports on its immune regulation. Therefore, it is necessary to Provide a method for extraction, purification and structure identification, laying the foundation for the quality control of Rhizoma Chuanxiong polysaccharide and in-depth study of its structure-activity relationship and mechanism of action

Method used

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  • Ligusticum wallichii polysaccharide as well as preparation method, identification method and application thereof
  • Ligusticum wallichii polysaccharide as well as preparation method, identification method and application thereof
  • Ligusticum wallichii polysaccharide as well as preparation method, identification method and application thereof

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Embodiment 1

[0051] The preparation method of embodiment 1 Chuanxiong polysaccharide

[0052] The preparation method of described Chuanxiong polysaccharide comprises the following steps:

[0053] S1 Cutting: Cut 10kg of the dried root of Rhizoma Chuanxiong to 1-3cm with scissors, wash it quickly with cold water, and dry it to obtain the Rhizoma Chuanxiong segment;

[0054] S2 water extraction: add water 10 times its weight to the Rhizoma Chuanxiong section obtained in step S1, heat to 80°C for extraction, extract for 3h, collect the extract, dry the dregs to obtain the extract and dregs;

[0055] S3 graded alcohol precipitation:

[0056] Concentrate the extract obtained in step S2 under reduced pressure at 60°C, add ethanol until the volume concentration of ethanol is 50%, let it stand at room temperature for 24 hours, centrifuge, collect the precipitate and supernatant, and obtain crude polysaccharide LC1 and supernatant 1;

[0057] After the supernatant 1 was concentrated under reduced...

Embodiment 2

[0060] Example 2 Ligusticum chuanxiong polysaccharide LC2-1-2A

[0061] Described Ligusticum chuanxiong polysaccharide LC2-1-2A is gained by secondary purification of the polysaccharide of Ligusticum chuanxiong obtained in Example 1, and the specific method is as follows:

[0062] 1) Ion-exchange column chromatography: take 200 mg of Ligusticum chuanxiong polysaccharide LC2 obtained in Example 1, dissolve it in 5 mL of deionized water, and load it on a DEAE-FF column. Two peaks appear under the eluent conditions of different salt concentrations, among which The elution peak is the 0.1M NaCl elution fraction (the elution curve is followed by the phenol-sulfuric acid method during the elution process, and the sugar fraction is collected according to the elution curve). A polysaccharide LC2;

[0063] 2) Molecular sieve gel chromatography: dissolve the above-mentioned freeze-dried polysaccharide sample in water, centrifuge, take the supernatant, put it on a Sephadex G-75 column, ...

Embodiment 3

[0064] Example 3 Structural Analysis of Ligusticum Chuanxiong Polysaccharide LC2-1-2A

[0065] (1) Test material: Ligusticum chuanxiong polysaccharide LC2-1-2A.

[0066] (2) Test method:

[0067] 1. Monosaccharide composition analysis

[0068] Sample handling:

[0069] Accurately weigh 4.0 mg of the test material of the Chuanxiong polysaccharide sample into a stoppered test tube, add 2.0 mL of 2M trifluoroacetic acid (TFA), and place it in an oil bath for hydrolysis at 120°C for 6 hours. Cool to room temperature, repeatedly add methanol and spin dry, remove TFA, dissolve to 1mL with deionized water, centrifuge, absorb 100μL sample solution, add 100μL 0.3M NaOH solution, then add 100μL 0.5M PMP methanol solution, mix well, 70 React in a water bath at ℃ for 30 minutes, cool down, add 105 μL of 0.3M HCL solution to neutralize, add deionized water to 1 mL, then add an equal volume of chloroform solution, shake vigorously, centrifuge, remove the chloroform phase, and repeat the ...

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Abstract

The invention belongs to the technical field of medicine and health food, and particularly relates to ligusticum wallichii polysaccharide as well as a preparation method, an identification method and application thereof. The molecular weight range of the ligusticum wallichii polysaccharide provided by the invention is 1000 to 100000 Da. The preparation method is simple, reaction conditions are mild, and large-scale production can be achieved; the chemical structure of the obtained high-purity ligusticum wallichii polysaccharide is identified, the structure of the ligusticum wallichii polysaccharide is defined, and a structural basis is provided for exploring the pharmacological activity mechanism of the ligusticum wallichii polysaccharide. Meanwhile, the obtained pure ligusticum wallichii polysaccharide lays a foundation for ligusticum wallichii polysaccharide medicines, health-care foods and functional foods, quality control of the ligusticum wallichii polysaccharide, and deep research of the structure-function relationship and the action mechanism of the ligusticum wallichii polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food, and in particular relates to a Chuanxiong polysaccharide and its preparation method, identification method and application. Background technique [0002] The immune organs and immune cells of the human body can protect the body from external invasion and protect human health. These immune structures can effectively improve the activity of immune cells, promote the secretion of antibodies by cytokines, and activate complement cells for immune regulation. The role of polysaccharides in immune regulation is mainly two aspects, non-specific immunity and specific immunity. Among them, specific immunity includes cellular immunity and humoral immunity. [0003] Polysaccharides, also known as polysaccharides, are a class of biological information macromolecules that widely exist in animals, plants and microorganisms. The research on polysaccharides has a history of nearly 70 years. Es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/02G01N30/02G01N30/06G01N30/74
CPCC08B37/006C08B37/0003G01N30/02G01N30/06G01N30/74A61P37/02G01N2030/067
Inventor 郭远强许婧张少杰安莉君
Owner NANKAI UNIV
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