Fermentation culture medium for producing tetracycline by fermenting streptomyces aureus and feeding method

A fermentation medium, Streptomyces aureus technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of high raw material costs, increased difficulty in waste residue treatment, and inability to upgrade fermentation units, etc. Achieve the effect of reducing viscosity, reducing starch consumption, and strengthening pathways

Inactive Publication Date: 2021-05-07
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology, the unit consumption of starch in the tetracycline fermentation process is 9-12 kg/billion tetracycline, which is a huge consumption
The fermentation process consumes a large amount of food crop

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The seed culture method was unchanged, and the fermentation culture conditions were unchanged.

[0035] Soybean oil methyl esterified oil was selected as the fermentation medium and feeding material, and the methyl ester rate was 81.2%.

[0036] The composition of the fermentation medium is: 2.3% starch, 0.2% methylated oil, 3% soybean cake powder, 0.25% yeast powder, 2.5% corn steep liquor, 0.65% ammonium sulfate, 0.45% light calcium carbonate, trace elements and inorganic salts Appropriate amount, the ratio of methyl esterified oil to starch in the base material is 1:8.7.

[0037] Starch sugar is added during the fermentation process, and the total sugar is reduced to 2.0%. The sugar supplement is started, and the total sugar is controlled at 1.5-2.5%.

[0038] Add ammonia water according to the pH, and start adding ammonia water when the pH drops to 6.0, and control the pH at 5.8-6.0.

[0039] Fermentation 24hrs began to feed soybean oil methyl esterified oil, the ...

Embodiment 2

[0042] The seed culture method was unchanged, and the fermentation culture conditions were unchanged.

[0043] Cottonseed oil methyl esterified oil was selected as the fermentation medium and feeding material, and the methyl ester rate was 84.5%.

[0044] The composition of the fermentation medium is: 4% starch, 0.4% methylated oil, 2% soybean cake powder, 2.2% corn steep liquor, 0.45% yeast powder, 0.75% ammonium sulfate, 0.55% light calcium carbonate, trace elements and inorganic salts Appropriate amount, the ratio of methyl esterified oil to starch in the base material is 1:10.

[0045] Starch sugar is added during the fermentation process, and the total sugar is reduced to 2.0%. The sugar supplement is started, and the total sugar is controlled at 1.5-2.5%.

[0046] Add ammonia water according to the pH, and start adding ammonia water when the pH drops to 6.0, and control the pH at 5.8-6.0.

[0047]Fermentation 25hrs began to feed cottonseed oil methyl esterified oil, th...

Embodiment 3

[0050] The seed culture method was unchanged, and the fermentation culture conditions were unchanged.

[0051] Rapeseed oil methyl esterified oil was selected as the fermentation medium and feeding material, and the methyl ester rate was 86.7%.

[0052] The composition of the fermentation medium is: 3% starch, 0.35% methylated oil, 2.5% soybean cake powder, 2.7% corn steep liquor, 0.3% yeast powder, 0.58% ammonium sulfate, 0.6% light calcium carbonate, trace elements and inorganic salts Appropriate amount, the ratio of methyl esterified oil to starch in the base material is 1:11.6.

[0053] Starch sugar is added during the fermentation process, and the total sugar is reduced to 2.0%. The sugar supplement is started, and the total sugar is controlled at 1.5-2.5%.

[0054] Add ammonia water according to the pH, and start adding ammonia water when the pH drops to 6.0, and control the pH at 5.8-6.0.

[0055] Fermentation 30hrs began to feed into rapeseed oil methyl esterified oi...

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PUM

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Abstract

The invention relates to a fermentation culture medium for producing tetracycline by fermenting streptomyces aureus and a feeding method. The fermentation culture medium and a feeding base material both adopt methyl esterified grease as an auxiliary carbon source. Methyl esterified grease is introduced and forms an auxiliary carbon source with starch, so that synthesis of fatty acid can be effectively prevented, a metabolic pathway of a product is enhanced, the fermentation level is improved, and the consumption of the starch is reduced.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a fermentation medium and feeding method for producing tetracycline by a Streptomyces aureus fermentation method. Background technique [0002] Tetracycline is a class of tetracyclic antibiotics, most of which are currently produced by fermentation. The fermentation process uses Streptomyces aureus as the fermentation strain and is produced in a two-stage fermentation mode. The specific process is: rejuvenate, separate, and cultivate the spores of Streptomyces aureus Prepare the slant spores for later use, then put the slant spores into the seed tank for seed expansion cultivation, and finally put the cultivated seeds into the fermentation medium, and control the growth of the bacteria by feeding the process, controlling the culture temperature, pH, matrix concentration, etc. Primary and secondary metabolism, and finally make the bacteria synthesize te...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12R1/49
CPCC12P29/00
Inventor 李学兵郭海燕孙瑞君张志胡晓玲胡钦龙冯占梅
Owner NINGXIA QIYUAN PHARMA
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