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Inactivated whole-virus influenza vaccine and method for preparing same

An influenza vaccine, whole particle technology, applied in the field of inactivated whole particle influenza vaccine, can solve the problem of low antibody induction

Pending Publication Date: 2021-05-04
DENKA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Split vaccine is a vaccine with excellent safety that reduces local reactions and febrile reactions, but there is a problem of low antibody induction in children with a history of influenza virus infection, children with a low vaccination history, and immunocompromised elderly people

Method used

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  • Inactivated whole-virus influenza vaccine and method for preparing same
  • Inactivated whole-virus influenza vaccine and method for preparing same
  • Inactivated whole-virus influenza vaccine and method for preparing same

Examples

Experimental program
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preparation example Construction

[0035] The preparation method of the inactivated whole particle influenza vaccine of the present invention utilizes the chicken embryo method and includes the process of performing hypotonic treatment.

[0036] The "chicken embryo method" refers to the method of inoculating the virus strain into hatching eggs for cultivation, clarifying, concentrating, purifying and inactivating the virus suspension to obtain a virus liquid containing virus particles.

[0037] Here, in the cultivation, the influenza virus strain is inoculated into hatching eggs, and it is performed at 30-37° C. for about 1 to 7 days, preferably at 33-35° C. for about 2 days. After the culture, the virus suspension (infected allantoic fluid) was collected and clarified by centrifugation or filtration. Next, it was concentrated by ultrafiltration. Virus purification can be performed by methods such as ultracentrifugation such as sucrose density gradient centrifugation, liquid chromatography, and the like.

[0...

Embodiment

[0053] Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited by these at all.

reference example 1

[0054] Reference Example 1 Isolation of extracellular vesicles contained in chorioallantoic fluid of uninfected eggs

[0055] Seventy-two 11-day-old chicken embryos were cooled at 4° C. for 1 hour or more, and chorioallantoic fluid was recovered from each chicken embryo using a disposable needle and syringe (manufactured by Terumo). The recovered chorioallantoic fluid was pooled and centrifuged at 4°C, 300×g for 10 minutes, and the obtained supernatant was centrifuged at 4°C, 141,000×g for 4 hours using an ultracentrifuge (manufactured by Hitachi Koki). The precipitate after ultracentrifugation was suspended in 6.7 mM phosphate-buffered saline (pH 7.2), and centrifuged again at 4° C. and 141,000×g for 4 hours. The obtained pellet was suspended in 6.7 mM phosphate-buffered saline (pH 7.2) to obtain an extracellular vesicle suspension.

[0056] 5 μL of a 0.1% poly-L-lysine solution was dropped onto a TEM electron microscope grid (manufactured by Oken Shoji Co., Ltd.) with a col...

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Abstract

Provided is an inactivated whole-virus influenza vaccine of which the antibody induction ability is maintained or enhanced and which causes reduced side reactions. A method for preparing the inactivated whole-virus influenza vaccine using an embryonated chick assay, comprising the step of subjecting a virus solution containing influenza virus whole particles collected from an embryonated chicken egg to a hypotonic treatment.

Description

technical field [0001] The invention relates to an inactivated whole particle influenza vaccine with high antibody inducing ability and reduced pyrogenicity and a preparation method thereof. Background technique [0002] Influenza viruses belong to the Orthomyxoviridae family and are classified into A, B, C, and D viruses due to the antigenic differences of the nucleoprotein and matrix protein present in the virus. Types A and B are prevalent every year, and sometimes they become severe due to viral infection, especially in children and the elderly. Influenza vaccination is known as a preventive method against influenza virus infection, and the influenza vaccine is a multivalent vaccine containing antigens of 2 strains of type A and 1 or 2 strains of type B. [0003] In Japan, although inactivated whole-particle vaccines have been used for influenza vaccines until 1971, the inactivated whole-particle vaccines are made by inoculating the vaccine strains in hatching eggs and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145A61K47/46A61P31/16
CPCA61P31/16A61K39/12C12N2760/16234A61K2039/5252A61K2039/575C12N2760/16134A61K2039/57A61K39/145C12N2760/16151C12N15/1131
Inventor 三股亮大郎三隅将吾岸本直树五反田卓摩中田渚
Owner DENKA CO LTD
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