Preparation method of nanoparticle bionic enzyme sensitive element, product and application of product

A technology of nanoparticles and sensitive components, applied in the field of materials, can solve the problems of elusive electronic structure and hinder understanding, and achieve the effect of detecting multiple active sites, enhancing selectivity, and improving electron transfer performance.

Pending Publication Date: 2021-05-04
SOUTHWEST UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high surface energy of such materials, how to construct such materials that are chemically stable remains a challenge
What's more, the electronic structure of atom-doped carbon nanomaterials decorated with metal nanoparticles is elusive, which hinders the understanding of such materials.

Method used

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  • Preparation method of nanoparticle bionic enzyme sensitive element, product and application of product
  • Preparation method of nanoparticle bionic enzyme sensitive element, product and application of product
  • Preparation method of nanoparticle bionic enzyme sensitive element, product and application of product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In the synthesis of nanoparticle biomimetic enzyme material (Co-NSG), the specific steps are as follows:

[0041] (1) First, 100 mg of graphite oxide (GO) was dispersed in 30 mL of deionized water (DIW) by ultrasound, and 2.5 mg of C 4 h 6 CoO 4 4(H 2 O); the mixture was stirred in a water bath at 80 °C for 2 hours, and then freeze-dried for 24 hours to remove DIW. Subsequently, the resulting product was mixed with 500 mg C 3 h 6 N 6 and 3g·L -1 C 3 h 7 NO 2 S were ground together to form a homogeneous mixture, then pyrolyzed at 900 °C for 2 h in an argon atmosphere. After cooling to room temperature, the biomimetic enzyme material (abbreviated as Co-NSG nanoparticle biomimetic enzyme material) is obtained.

[0042] Working electrode coated with biomimetic enzyme sensitive element:

[0043] The Co-NSG nanoparticle biomimetic enzyme material prepared above was dispersed in water at a concentration of 1 mg / mL to obtain an electrode modification solution, and 4...

Embodiment 2

[0052] Superoxide dismutase biomimetic enzyme material, the specific steps are as follows:

[0053] Mix 100mg GO with 500mg C 3 h 6 N 6 and 3g·L -1 C 3 h 7 NO 2 S were ground together into a homogeneous mixture and pyrolyzed at 1000 °C for 2 h in an argon atmosphere. After cooling to room temperature, the biomimetic enzyme material (abbreviated as NSG biomimetic enzyme material) is obtained;

[0054] Working electrode coated with NSG biomimetic enzyme material:

[0055] The prepared NSG biomimetic enzyme material was dispersed in water at a concentration of 1 mg / mL to obtain an electrode modification solution, which was coated on a glassy carbon electrode, dried at 26°C for 5 hours, and then coated with Nafion mass fraction 0.1 wt% Nafion solution, and dried again at 26° C. for 5 h to prepare a working electrode coated with superoxide dismutase biomimetic material.

[0056] Construction of superoxide anion radical electrochemical sensor:

[0057] The working electrod...

Embodiment 3

[0061] Superoxide dismutase biomimetic enzyme material, the specific steps are as follows:

[0062] Prepare 45mL of phosphate buffer solution, then add 0.1014g of manganese sulfate into 2mL of deionized water, mix the two solutions evenly, stir and react at 26°C for 8h, centrifuge at 5000r / min for 10min, and take the precipitate. After washing with water, vacuum-dry at 80° C. for 12 hours to prepare superoxide dismutase biomimetic enzyme material.

[0063] Working electrode coated with biomimetic enzyme sensitive element:

[0064] Disperse the prepared superoxide dismutase biomimetic material in water at a concentration of 1.5 mg / mL to obtain an electrode modification solution, apply the electrode modification solution to a glassy carbon electrode, dry at 26°C for 5 hours, and then apply Cover with a Nafion solution with a mass fraction of Nafion of 0.1 wt%, and dry it again at 26° C. for 5 hours to prepare a working electrode whose surface is coated with a superoxide dismuta...

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Abstract

The invention discloses a preparation method of a nanoparticle bionic enzyme sensitive element, a product and application of the nanoparticle bionic enzyme sensitive element. The method includes: dispersing a two-dimensional nanomaterial in a solvent, adding acetate containing a transition metal source, performing water bath for a certain time at a certain temperature, and conducting freeze drying to remove the solvent; grinding the obtained product, a six-membered heterocyclic compound and a substance amino acid into a uniform mixture, then performing pyrolyzing in an inert atmosphere, and conducting cooling to obtain the nanoparticle bionic enzyme sensitive element. After the prepared sensitive element is prepared into a sensor, the sensor not only has extremely short response time, extremely high reaction sensitivity and relatively low detection limit, but also has excellent selectivity; the material has an important application prospect in the aspect of real-time detection of superoxide anion free radicals released by living cells, and is outstanding in performance, convenient in material taking and beneficial to commercial application.

Description

technical field [0001] The invention relates to the field of material technology, in particular to a method for preparing a nanoparticle biomimetic enzyme sensitive element, and also relates to the product and application of the method, as well as an electrochemical sensor prepared by using the nanoparticle biomimetic enzyme sensitive element. Background technique [0002] Superoxide anion (O 2 .- ) is the product of the reduction of oxygen molecules by a single electron, and is also the first free radical formed by cells in the process of oxygen metabolism. All other reactive oxygen species (ROS) are derived from O 2 .- Derived from. o 2 .- Concentration fluctuations are closely related to the occurrence and development of many biological processes and diseases. Under normal physiological conditions, O 2 .- The concentration in cells will be controlled in a low range and maintain a relatively stable dynamic balance, which can assist cells in normal growth and metabo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/404
CPCG01N27/404
Inventor 李长明邹卓杨鸿斌
Owner SOUTHWEST UNIV
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