Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Beauveria bassiana, application of Beauveria bassiana, complex microbial inoculant and special disease-resistant and insect-resistant yield-increasing compound fertilizer for Korean pine nut forest

A technology of Beauveria bassiana and compound bacterial agent, which is applied in the field of agricultural and forestry microorganisms, can solve problems such as heavy workload, difficult control of diseases and insect pests, and complicated operations

Active Publication Date: 2021-04-30
NORTHEAST FORESTRY UNIVERSITY +2
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of difficult prevention and control of existing Korean pine forest diseases and insect pests, complicated operations, heavy workload and high cost, the present invention provides a strain of Beauveria bassiana and its application, compound bacterial agent and special disease-resistant and pest-resistant compound for increasing production of Korean pine nut forests. Fat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Beauveria bassiana, application of Beauveria bassiana, complex microbial inoculant and special disease-resistant and insect-resistant yield-increasing compound fertilizer for Korean pine nut forest
  • Beauveria bassiana, application of Beauveria bassiana, complex microbial inoculant and special disease-resistant and insect-resistant yield-increasing compound fertilizer for Korean pine nut forest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This example provides a strain of Beauveria bassiana SBM03.

[0026] In this embodiment, from the 50-year-old Korean pine plantation in the suburbs of Jiulong Village, Boli County, Qitaihe City, Heilongjiang Province, a microorganism with parasitic and lethal effects was obtained by separating and purifying from the corpse of the larvae of the fir spruce moth. Its serial number is SBM03. The morphological characteristics, physiological and biochemical characteristics and molecular biological identification of the strain were carried out, and the results are as follows:

[0027] (1) Morphological characteristics

[0028] figure 1 It is the colony morphology diagram of Beauveria bassiana SBM03 of the present invention. Such as figure 1 As shown, the colony of Beauveria bassiana SBM03 is round, pure white and velvet in the early stage of growth, and milky white or light milky yellow after sporulation in the later stage, powdery, and the particle size of the spores prod...

Embodiment 2

[0033] In this example, the infection, parasitism and lethality of Beauveria bassiana SBM03 in a low temperature environment were investigated. Beauveria bassiana SBM03 obtained in Example 1 and other commercially available Beauveria bassiana strains, i.e. numbers bio-13854, bio-13827, and bio-25400 on Beijing Biobowell Biotechnology Co., Ltd.-China Microbial Species Inquiry Platform , bio-23839, bio-25812, bio-25803 Beauveria bassiana, in the environment of 20 ℃, 22 ℃, 24 ℃, the third instar larvae of the spot moth moth was infected and parasitic lethal experiment. Table 1 shows 10 at constant temperature 8 The spore / L spore suspension was quantitatively sprayed and cultured at constant temperature for 14 days, and the mortality rate of each experimental group was corrected.

[0034] Table 1

[0035]

[0036] 20°C, 22°C, and 24°C are all lower than the optimum ambient temperature for the reproduction of Beauveria bassiana. As can be seen from the data in Table 1, compared ...

Embodiment 3

[0039] This embodiment provides a composite bacterial agent, comprising the following components in mass percentage: 30-40% of the master drug of Bacillus subtilis, 40-50% of the master drug of Beauveria bassiana SBM03 spore powder, and 10% of water-soluble starch. ~20% and glucose powder 10~20%. Among them, the amount of spores contained in the parent drug of Bacillus subtilis is 50-100 billion spores / g; the amount of spores contained in the master drug of Beauveria bassiana SBM03 spore powder is 5-1 billion spores / g.

[0040] In the compound microbial agent provided in this example, Bacillus subtilis can be well attached to the roots of plants, competitively inhibit the reproduction of harmful fungi and bacteria, and prevent diseases such as root rot; Beauveria bassiana SBM03 can be parasitic Kill a variety of Lepidoptera pests, realize pollution-free and sustainable control of the population density of pests, and suppress the prevalence of pest disasters. Because Beauveria...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to beauveria bassiana and an application thereof, a complex microbial inoculant and a special disease-resistant and insect-resistant yield-increasing compound fertilizer for Korean pine nut forests, and belongs to the technical field of agriculture and forestry microorganisms. In order to solve the problems of high difficulty and high cost of disease and pest control of Korean pine forests, the invention provides a beauveria bassiana SBM03 which has stronger infection, parasitism and lethal ability to lepidoptera insects at low temperature, and can solve the problems of weak parasitism and poor insecticidal effect of the existing beauveria bassiana at low temperature. The invention also provides a composite microbial agent containing beauveria bassiana SBM03, which can realize pollution-free and sustainable control of population density of pests and inhibit prevalence of insect disasters. The special disease-resistant and insect-resistant yield-increasing compound fertilizer for the Korean pine nut forest, provided by the invention, comprises a basic base fertilizer, trace elements and a compound microbial agent containing beauveria bassiana SBM03, the aims of disease resistance, insect resistance and yield increase are simultaneously achieved through reasonable fertilization, and the problems of high disease and insect prevention and control cost and low Korean pine nut yield of the Korean pine forest are solved.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry microorganisms, and in particular relates to a strain of Beauveria bassiana and its application, a compound bacterial agent and a special disease-resistant and insect-resistant compound fertilizer for increasing production of Korean pine nut forests. Background technique [0002] Korean pine is a national second-class protected plant, mainly distributed in the Changbai Mountains and Xiaoxing'anling forest areas in Northeast my country. Korean pine nuts are the seeds of Korean pine. They are rich in protein, carbohydrates, and fat. Most of the fat is unsaturated fatty acids such as oleic acid and linoleic acid. It also contains trace elements such as calcium, phosphorus, and iron. It is a wood with rich nutritional value. The oil and health food. Regular consumption of red pine nuts can prevent heart disease, lower blood lipids, soften blood vessels, and have anti-cancer and anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C05G3/60C12R1/645
CPCC12N1/14A01N63/30C05G3/60C05B1/04C05C3/00C05D1/02C05D9/02C05F11/00
Inventor 郭鸿儒迟德富贾妮娅朱天博石春玲宇佳
Owner NORTHEAST FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com