Method for extracting melanin from cuttlefish juice

A technology for ink melanin and cuttlefish juice, which is applied in the field of pigment extraction technology, can solve problems such as influence differentiation, and achieve the effects of preserving integrity, simple operation and high extraction rate.

Active Publication Date: 2021-04-09
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the raw material also has an important influence on the type of extracted substance (eumelanin or phenomelanin) obtained at last, which will affect the subsequent distinction

Method used

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  • Method for extracting melanin from cuttlefish juice
  • Method for extracting melanin from cuttlefish juice
  • Method for extracting melanin from cuttlefish juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The melanin was extracted by alkali extraction and acid precipitation, 5.0 g of cuttlefish juice powder was weighed, 0.5-2.5 mol / L NaOH solution was added according to the solid-to-liquid ratio of 1:10-50 (g / mL), and placed at 30-70 ℃ After leaching in water bath for 1-3h, 8000r·min －1 Centrifuge for 10min, take the supernatant, adjust the pH value to 1-3 with 6.00mol / L HCl solution, after standing, 10000r·min －1 Centrifuge for 15min, take the precipitate and wash with water, 15000r·min －1 Centrifuge for 5 min, repeat the operation 3 times, freeze-dry the precipitate, and calculate the extraction rate (see Table 1-5).

[0028] Table 1 Effect of NaOH concentration on the extraction rate of melanin

[0029] NaOH concentration (mol / L) Extraction grams (g) Extraction rate (%) 0.5 0.8609 17.22 1.0 1.1299 22.60 1.5 0.7293 14.59 2.0 0.6327 12.65 2.5 0.3467 6.93

[0030] Note: The solid-liquid ratio is 1:20, the temperature is ...

Embodiment 2

[0052] The raw material pretreatment was the same as that in Example 1, and a dry cuttlefish juice powder was obtained. Take the extraction of melanin with alkaline protease as an example.

[0053] Cuttlefish juice powder and alkaline solution with pH value of 9-11 are mixed according to the ratio of solid-to-liquid (g / ml) ratio of 1:3-100, and alkaline protease is added, and the amount of enzyme added is 60,000-180,000 U / g. Enzymatic hydrolysis was carried out at 40-60°C for 3-5h, and the reaction time was 6000r·min after the reaction. -1 Centrifuge for 10 min, remove the supernatant after centrifugation, wash the precipitate with distilled water and centrifuge, repeat the operation 3 times, get the melanin precipitate and place it in a -80°C ultra-low temperature freezer for freezing, and place it in a vacuum freeze dryer to freeze-dry to obtain melanin powder. After freeze-drying, the extraction rate was calculated (see Table 8-12), and it was stored in a refrigerator at 4...

Embodiment 3

[0082] Validation study of the melanin extracted in Example 3

[0083] 1, the above-mentioned embodiment is extracted gained melanin and standard substance to carry out infrared spectrum analysis:

[0084] Weigh the fully ground purchased standard samples, enzyme-extracted melanin samples, and alkali-extracted melanin samples, place them in the center of a clean disc, rotate and compact the samples, and use a Fourier transform infrared spectrometer to detect the infrared spectra of the samples respectively. . (see Picture 1-1 , 1-2, 1-3)

[0085] 2, the above-mentioned embodiment is extracted gained melanin and standard substance to carry out ultraviolet maximum absorption wavelength scanning:

[0086] The UV-Vis absorption spectrum has a high degree of specificity, that is, the energy absorbed by a molecule of a certain structure is certain, and the change of its structure can be judged by studying the change of the UV-Vis absorption spectrum of macromolecules. Dissolve 0...

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Abstract

The invention relates to the field of pigment extraction processes, in particular to a method for extracting melanin from cuttlefish juice by adopting enzymes. The method specifically comprises the following steps: filtering cuttlefish juice to remove impurities, drying or freeze-drying to obtain powder, and extracting melanin by using enzyme. The ink melanin obtained by adopting the method disclosed by the invention is high in extraction rate and good in purity. The method is easy to operate, environmentally friendly, efficient and suitable for large-scale operation of extracting high-purity melanin from cuttlefish juice.

Description

technical field [0001] The invention relates to the technical field of pigment extraction, in particular to a method for extracting melanin from cuttlefish juice by using an enzyme. Background technique [0002] Melanin is a kind of important natural pigment that widely exists in nature, mainly distributed in animals, plants and microorganisms. Melanin is a complex and diverse macromolecular substance polymerized by polyhydroxyindole or polyhydroxyphenol. It is often combined with proteins and other substances and exists in cells. There are two types of animal melanin ubiquitous in nature, one is eumelanin and the other is demelanin. Different species of melanin have different differences. The scientific name of cuttlefish is also called squid, and the melanin in ink sac ink is mainly eumelanin. Eumelanin is a two-molecule indole structure formed by the oxidation of tyrosine, namely DHI (5,6-dihydroxyindole) and DHICA (5,6-dihydroxyindole acid), so melanin is water-insolu...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 邢荣娥宋雯杨皓月刘松于华华李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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