Lactobacillus rhamnosus CCFM1131 for relieving hyperuricemia
A technology of CCFM1131 and Lactobacillus rhamnosus, applied in the field of microbiology, can solve problems such as undiscovered, and achieve the effects of promoting expression, reducing gout, and lowering serum uric acid levels
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Embodiment 1
[0046] Example 1: Screening of Lactobacillus rhamnosus CCFM1131
[0047] (1) Isolation and screening of Lactobacillus
[0048] (1) Take 1g of fresh feces of healthy adults. After gradient dilution, it was applied to LBS medium supplemented with 1% nystatin, and placed in a constant temperature incubator at 37°C for 48 hours.
[0049] (2) After cultivation, according to the color, size, and edge shape of the colony, use an inoculation loop to pick up the colony and streak it for purification.
[0050] (3) The obtained colonies were subjected to Gram staining and catalase analysis.
[0051] (4) Retain Gram-positive bacilli and catalase-negative bacteria.
[0052] (2) Molecular biological identification of Lactobacillus
[0053] (1) Single bacterial genome extraction
[0054] (A) cultivating the lactobacilli screened in step (1) overnight;
[0055] (B) Take 1 mL of the bacterial suspension cultivated overnight in a 1.5 mL centrifuge tube, centrifuge at 10000 r / min for 2 min...
Embodiment 2
[0071] Example 2: Lactobacillus rhamnosus CCFM1131 has good tolerance to simulated gastrointestinal fluid
[0072] The cryopreserved Lactobacillus rhamnosus CCFM1131 was inoculated in MRS medium, cultured at 37° C. for 14 hours, and subcultured in MRS medium for 2 to 3 times.
[0073] Take 3mL of the culture solution of Lactobacillus rhamnosus CCFM1131 and centrifuge at 8000×g for 2min to collect the bacteria, mix it with 3mL pH 3.0 artificial simulated gastric juice (containing 3g / L pepsin, pH=3.0 physiological saline), and incubate in a 37°C incubator Cultivate, take samples at 0h and 2h respectively, use MRS agar medium to pour culture, count the colonies on the plate, determine the number of viable bacteria and calculate their survival rate.
[0074] Take 3 mL of the culture solution of Lactobacillus rhamnosus CCFM1131 and centrifuge at 8000×g for 2 min to collect the cells, add 3 mL of pH8.0 artificial simulated intestinal fluid (physiological saline containing 1 g / L tryp...
Embodiment 3
[0078] Example 3: Lactobacillus rhamnosus CCFM1131 has no toxic side effects on KunMing mice
[0079] Resuspend Lactobacillus rhamnosus CCFM1131 in 100g / L skim milk solution to make a concentration of 4.0×10 9 CFU / mL bacterial suspension. Twelve healthy male KunMing mice with a body weight of about 24-32 g were taken, and after one week of adaptation to the environment, they were divided into CCFM1131 group and control group. The CCFM1131 group was given this concentration of bacterial suspension 0.3mL by intragastric administration once a day, and the control group was intragastrically administered the same volume of 100g / L skim milk solution without Lactobacillus rhamnosus CCFM1131, observed for one week, and recorded death and body weight.
[0080] The results of these tests are listed in Table 2. These results indicate that feeding concentrations of 1×10 9 CFU / Lactobacillus rhamnosus CCFM1131 had no significant impact on mice, no significant change in body weight, and n...
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