Preparation method of natto extract rich in spermidine

The technology of spermidine and extract is applied in the field of preparation of natto extract, and achieves the effects of simple process, considerable economic benefit, and avoiding thermal degradation.

Inactive Publication Date: 2021-03-26
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for extracting spermidine using supercritical carbon dioxide fluid extraction technology in the prior art

Method used

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  • Preparation method of natto extract rich in spermidine
  • Preparation method of natto extract rich in spermidine
  • Preparation method of natto extract rich in spermidine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: use the inventive method to prepare the natto extract rich in spermidine

[0035] (1) Take 5kg of solid-state fermented natto for freeze-drying, use micro-crushing equipment to pulverize the freeze-dried natto, and sieve it through a 200-mesh sieve; the freeze-drying conditions are: the temperature of the freeze-drying box is - 50°C, the vacuum degree is 30Pa, the shelf heating temperature is 30-50°C, and the drying time is 20 hours, the weight of the obtained crushed raw material powder is 1.66kg;

[0036] (2) Take 230g of pulverized raw material powder and put it into a 1.0L extraction kettle, prepare 500mL of 30% ethanol aqueous solution and add it to the carrier tank, open the supercritical carbon dioxide fluid extraction device, and heat and pressurize the carbon dioxide in the device to make it Reach the supercritical fluid state, then turn on the carrier pump, and extract the spermidine in the raw material powder with the supercritical carbon dioxid...

Embodiment 2

[0054] Embodiment 2: use the inventive method to prepare the natto extract rich in spermidine

[0055] (1) Take 230g of the pulverized raw material powder in the above example and put it into a 1.0L extraction kettle, prepare 500mL of 25% ethanol aqueous solution and add it to the carrier tank, open the supercritical carbon dioxide fluid extraction device, and heat the carbon dioxide in the device pressure to make it reach the supercritical fluid state, and then open the carrier pump to extract the spermidine in the raw material powder with the supercritical carbon dioxide fluid mixed with the carrier; the supercritical carbon dioxide fluid extraction condition is: the temperature of the extraction kettle is 40 ℃, the pressure of the extraction kettle is 30MPa, the extraction time is 40min, the flow rate of carbon dioxide is 20L / h, the water-containing ethanol is used as the carrier, and the flow rate of the carrier pump is 10mL / min;

[0056] (2) The supercritical carbon dioxi...

Embodiment 3

[0058] Embodiment 3: use the inventive method to prepare the natto extract rich in spermidine

[0059] (1) Take 230g of the pulverized raw material powder in the above example and put it into a 1.0L extraction kettle, prepare 500mL of 20% ethanol aqueous solution and add it to the carrier tank, open the supercritical carbon dioxide fluid extraction device, and heat the carbon dioxide in the device pressure to make it reach the supercritical fluid state, and then open the carrier pump to extract the spermidine in the raw material powder with the supercritical carbon dioxide fluid mixed with the carrier; the supercritical carbon dioxide fluid extraction condition is: the temperature of the extraction kettle is 40 ℃, the pressure of the extraction kettle is 30MPa, the extraction time is 40min, the flow rate of carbon dioxide is 20L / h, the water-containing ethanol is used as the carrier, and the flow rate of the carrier pump is 10mL / min;

[0060] (2) The supercritical carbon dioxi...

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Abstract

The invention relates to a preparation method of a natto extract rich in spermidine. The preparation method is characterized in that the natto extract rich in spermidine is prepared from solid-state fermented natto by using a supercritical carbon dioxide fluid extraction technology. The method is simple in processing course, short in preparation time, high in extraction rate and low in extractiontemperature, and can effectively avoid thermal degradation of heat-sensitive components; the content of the spermidine in the obtained natto extract is 10-15 times that of natto. The natto extract does not contain thalli or spores, does not have unpleasant ammonia smell in fresh natto or natto freeze-dried powder, and can be accepted by more people. In the preparation process, waste residues, waste liquid and waste gas are not generated, nattokinase and other nutritional ingredients contained in the fresh natto are still retained in the extraction residues, further utilization is not affected,and the best use of the natto is achieved.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of natto extract rich in spermidine. Background technique [0002] Spermidine, H 2 N(CH 2 ) 3 NH(CH 2 ) 4 NH 2 , whose structural formula is shown in Formula I, is commonly found in animal, plant and microbial cells, and is biosynthesized from putrescine (butanediamine) and adenosylmethionine. [0003] [0004] Spermidine is a positively charged alkylamine polycation, which can easily react with negatively charged DNA, RNA and lipids, so it participates in many important life processes, such as regulating cell proliferation, cell aging, Physiological and pathological processes such as organ development, immunity, and cancer. Studies have shown that the disturbance of spermidine metabolism is related to the pathogenesis of various diseases. At the cytological level, spermidine can significantly prolong the lifespan and survival rate of yeast...

Claims

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Application Information

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IPC IPC(8): A23L11/50
Inventor 孙华庚
Owner 天津市尖峰天然产物研究开发有限公司
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