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Preparation method of fishskin collagen with certain-range molecular weight

A collagen and molecular weight technology, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of large molecular weight of peptides and cannot meet the needs, and achieve good water solubility, increased oil content, oil The effect of improved status

Inactive Publication Date: 2021-03-19
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing fish skin collagen with a certain range of molecular weight, so as to solve the problem in the above-mentioned background technology that the molecular weight of the currently prepared collagen polypeptide is too large to meet the demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing fish skin collagen with a certain range of molecular weight, the specific steps are as follows:

[0035] S1: fish skin pretreatment;

[0036] S2: enzymatic membrane separation;

[0037] S3: decolorization and deodorization treatment;

[0038] S4: Freeze drying.

[0039] The concrete steps of the fish skin pretreatment in the present embodiment are as follows:

[0040] S11: Take fresh fish skin and fish scales and wash them, add 20 times the volume of cleaning agent to it, control the temperature and soak at 4°C for 8-10 hours, remove fat and other non-collagen components in the fish skin, and rinse with distilled water until neutral;

[0041] S12: adding liquid nitrogen to the treated fish skin and fish scales and grinding them into powder.

[0042] Specifically, the cleaning agent uses NaOH with a concentration of 0.1mol / L and NaOH with a concentration of 0.05mol / L. 2 CO 3 Mixture.

[0043] The specific steps of enzymatic membrane circulat...

Embodiment 2

[0057] A method for preparing fish skin collagen with a certain range of molecular weight, the specific steps are as follows:

[0058] S1: fish skin pretreatment;

[0059] S2: enzymatic membrane separation;

[0060] S3: decolorization and deodorization treatment;

[0061] S4: Freeze drying.

[0062] The concrete steps of the fish skin pretreatment in the present embodiment are as follows:

[0063] S11: Take fresh fish skin and fish scales and wash them, add 23 times the volume of cleaning agent to it, control the temperature and soak at 5°C for 9 hours, remove fat and other non-collagen components in the fish skin, and rinse with distilled water until neutral;

[0064] S12: adding liquid nitrogen to the treated fish skin and fish scales and grinding them into powder.

[0065] Specifically, the cleaning agent uses NaOH with a concentration of 0.09mol / L and NaOH with a concentration of 0.04mol / L. 2 CO 3 Mixture.

[0066] The specific steps of enzymatic membrane circulatio...

Embodiment 3

[0080] A method for preparing fish skin collagen with a certain range of molecular weight, the specific steps are as follows:

[0081] S1: fish skin pretreatment;

[0082] S2: enzymatic membrane separation;

[0083] S3: decolorization and deodorization treatment;

[0084] S4: Freeze drying.

[0085] The concrete steps of the fish skin pretreatment in the present embodiment are as follows:

[0086] S11: Wash fresh fish skin and scales, add 25 times the volume of cleaning agent to it, control the temperature and soak at 8°C for 8 hours, remove fat and other non-collagen components in the fish skin, and rinse with distilled water until neutral;

[0087] S12: adding liquid nitrogen to the treated fish skin and fish scales and grinding them into powder.

[0088] Specifically, the cleaning agent uses NaOH with a concentration of 0.08mol / L and NaOH with a concentration of 0.03mol / L. 2 CO 3 Mixture.

[0089] The specific steps of enzymatic membrane circulation separation in the...

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Abstract

The invention relates to the technical field of collagen production, in particular to a preparation method of fishskin collagen with certain-range molecular weight. The preparation method specificallycomprises the following steps of: preprocessing fishskin, carrying out enzymolysis membrane cycling separation, carrying out decoloration and fishy smell elimination processing, and carrying out freeze drying. Through specific combined technical steps and parameter conditions, fishskin preprocessing, enzymolysis membrane cycling separation, decoloration and fishy smell elimination processing andfreeze drying are carried out to obtain collagen powder of which the molecular weight is 2-5KD, the triple helix structure of the prepared fishskin collagen is not damaged, and original biological activity is still kept; main ingredients are the 2-5KD collagen powder which has good water solubility and can be diluted by water at any ratio; the fishskin collagen is from the collagen of vertebrates,has good homology and compatibility with the muscle body and the skin of people and has similar molecular weight and structure with the people; and after the fishskin collagen is smeared to the skin,the fishskin collagen can be quickly dissolved into the epidermis, and collagen polypeptide of which the molecular weight is smaller than 5kDa has various physiological activities, including blood pressure reduction and oxidization resistance.

Description

technical field [0001] The invention relates to the technical field of collagen production, in particular to a method for preparing fish skin collagen with a certain molecular weight. Background technique [0002] Collagen peptide is a hydrolyzed product of collagen, which is widely used in food, medicine, chemical industry and other fields because of its low molecular weight, easy absorption, and certain biological activity. At present, the raw materials for the production of collagen products are basically the skin and bones of terrestrial animals such as pigs and cattle. Due to the scare of mad cow disease and other reasons, the use of collagen and its products is limited. Finding collagen raw materials other than pig, cowhide and bone has become a top priority, and aquatic product processing waste, especially fish skin and fish scales, is an ideal alternative raw material. As a large aquatic product processing country, my country produces a large amount of leftovers suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14C07K1/34
CPCC12P21/06C07K14/78
Inventor 牛海涛陈元滨王永华李丹
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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