Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Purification of human myocardium troponin I and preparation method of its monoclonal anti-body

A cardiac troponin and monoclonal antibody technology, applied in the direction of anti-animal/human immunoglobulin, animal/human peptide, peptide source, etc., can solve the problems of fighting for diagnosis and treatment time, unfavorable disease patients, and high price. It achieves the effects of fast measurement time, easy popularization and application, and low cost

Inactive Publication Date: 2006-03-29
上海润东生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In foreign countries, the immunoenzyme detection method is mainly completed by using automatic analyzers (such as Status, Access and other automatic analyzers), and the results can be obtained within 1 hour, but it is necessary to purchase supporting reagents such as cellulose acetate membranes coated with antibodies, and this type of Reagents are expensive
ELISA double-sandwich quantitative method is also used for detection, but this method is generally operated manually and takes a long time, which is not conducive to gaining valuable time for diagnosis and treatment of patients with diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Below in conjunction with embodiment above-mentioned method of the present invention is described in further detail:

[0027] (1) the present invention to the purification method of human cardiac troponin I:

[0028]1. Purification of human cardiac troponin C (cTnC)

[0029] (1) Take 100g of human ventricular muscle tissue fresh or stored at -75°C after liquid nitrogen quick-frozen, remove fat and fibers and shred, add 3 to 5 times the volume of homogenization buffer (Tris-HCl 50mmol / L, KCl 50mmol / L L, EDTA 1mmol / L, pH7.5) was fully homogenized, centrifuged for 30 minutes, the supernatant was removed, the precipitate was taken, and the above steps were repeated 10 times; fully homogenized each time. The precipitate was extracted more than 2 times with ethanol and ether, and dried into a dry powder. Dissolve the dry powder in buffer solution 1mol / L KCl for 12-24 hours, centrifuge at 10000g for 30 minutes, remove the precipitate, and take the supernatant;

[0030] (2) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a method for purifying human myocardium troporin I, preparing its monoclonal antibody and determining its content by using one-step process. It adopts affinity chromatography to purity human myocardium troponin I, and utilizes the purified myocardium troponin I and BALB / c mouse to immunoprepare the monoclonal antibody of said myocarbium troponin I, and establishes a method for determining myocardium troponin I content by means of one-step process. It can be extensively used for diagnosing heart disease.

Description

technical field [0001] The invention relates to a method for purifying human cardiac troponin I by affinity chromatography, a method for preparing a monoclonal antibody of the cardiac troponin I and a method for determining its content by one-step method. Background technique [0002] Myocardial ischemic injury, especially acute myocardial infarction (AMI) is one of the major diseases that threaten human life. Clinically, efforts have been made to find serum indicators with good specificity and high sensitivity to diagnose it. At present, creatine kinase isoformic acid (CK-MB) has been used as an important indicator for the diagnosis of myocardial infarction, but CK-MB is not unique to the heart, and also exists in a small amount in skeletal muscle of normal people. Cardiac surgery or skeletal muscle injury patients often have increased CK-MB, which can easily lead to false positive diagnosis of AMI. Recent studies have shown that cardiac troponin I (cardiac troponin I, cT...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K16/18C12P21/08
Inventor 张寄南苏恩本
Owner 上海润东生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com