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Mytilus crassitesta oligopeptide and use thereof

A thick-shelled mussel and oligopeptide technology, applied in the direction of peptide, peptide source, application, etc., can solve problems such as angioedema, rash, azotemia, death, etc., and achieve no cytotoxicity and high ACE inhibitory activity Effect

Inactive Publication Date: 2021-03-09
舟山市和朴海洋生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the antihypertensive drugs on the market mainly include amiloride, furosemide, clonidine, losartan, etc. These drugs will also bring great side effects to the human body when exerting their own curative effects. Such as cough, dysgeusia, azotemia, angioedema and rash, etc., and even death in severe cases

Method used

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  • Mytilus crassitesta oligopeptide and use thereof
  • Mytilus crassitesta oligopeptide and use thereof
  • Mytilus crassitesta oligopeptide and use thereof

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preparation example Construction

[0023] Embodiments of the present disclosure also provide a method for preparing mussel oligopeptides, including:

[0024] 1) Thaw the frozen thick-shelled mussel meat under running water at room temperature, clean it, dry the surface moisture at low temperature, dissect and mince it, add ethyl acetate to soak for 24-72 hours, stir continuously during the degreasing process, and vacuum filter the degreased sample After removing ethyl acetate (recovered by rotary steaming), dry and pulverize to obtain thick-shell mussel powder;

[0025] 2) According to the ratio of material to liquid: 1:20-60, add water to the thick-shelled mussel powder, then add 1-5% trypsin, enzymolyze at pH 7-9, 30-50°C for 3.0-5.0h, and the reaction is over Finally, inactivate the enzyme to obtain the enzymatic solution;

[0026] 3) Selecting an ultrafiltration membrane with a molecular weight cut-off of 1kDa to perform ultrafiltration on the mussel enzymatic hydrolyzate obtained in step 2) to obtain a <1...

Embodiment 1

[0032] A preparation method of mussel oligopeptide, comprising:

[0033] 1) Thaw the frozen thick-shell mussel meat under running water at room temperature, clean it, dry the surface moisture at low temperature, dissect and mince it, soak it in ethyl acetate for 48 hours, stir continuously during the degreasing process, and vacuum filter the degreased sample to remove acetic acid After the ethyl ester (recovered by rotary steaming), it is dried and pulverized to obtain thick-shelled mussel powder;

[0034] 2) Add water to the thick-shelled mussel powder according to the ratio of material to liquid 1:40, then add 3% trypsin, and enzymolyze it at pH 8 and 40°C for 4.0 hours, and heat it in boiling water at 100°C after the reaction Inactivate the enzyme for 10 minutes to obtain the enzymatic solution;

[0035] 3) Selecting an ultrafiltration membrane with a molecular weight cut-off of 1kDa to perform ultrafiltration on the mussel enzymatic hydrolyzate obtained in step 2) to obta...

Embodiment 2

[0045] Test of ACE Inhibitory Activity of Oligopeptides from Mussel mussels

[0046] Main reagents: hydrochloric acid (analytical pure), sodium chloride (analytical pure), sodium hydroxide (analytical pure), purchased from Sinopharm Chemical Reagent Co., Ltd.; N-2-hydroxyethylpiperazine-N'-2-B Sulfonic acid (HEPES) was purchased from Solarbio; angiotensin-converting enzyme (ACE) and FAPGG (N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly) were purchased from Sigma, USA.

[0047] The preparation of reagent: 80mM HEPES buffer solution (pH 8.3, Cl － Concentration: 300mM): HEPES 1.910g, NaCl 1.755g, dissolved in double-distilled water, adjusted pH to 8.3 with NaOH, added water to 100mL, set at 4°C for later use; FAPGG solution (1mM): Take 3.994mg FAPGG powder and add HEPES buffer , mix and dissolve, dilute to 10mL, and store at 4°C in the dark; ACE inhibitor (peptide to be tested): weigh an appropriate amount of active peptide powder, and use HEPES buffer as a solvent to prepare a series of a...

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Abstract

The invention provides a mytilus crassitesta oligopeptide and use thereof and belongs to the technical field of bioactive peptides. An amino acid sequence of the mytilus crassitesta oligopeptide is Leu-Ala-Ala-Ser-His or Tyr-Glu-Leu-His-Asp. The mytilus crassitesta oligopeptide provided by the invention has relatively high ACE inhibiting activity and is free of cytotoxicity, the content of an endogenous dilatation factor NO in blood vessel endothelial cells (HUVEC cells) can be increased, the content of an endogenous contraction factor ET-1 can be decreased, namely, the pressure release actionis exerted through affecting functions of the blood vessel endothelial cells. The mytilus crassitesta oligopeptide provided by the invention can be applied to preparation of health-care foods and / ormedicines with a blood pressure regulating action.

Description

technical field [0001] The invention belongs to the technical field of bioactive peptides, and in particular relates to a mussel oligopeptide and its application. Background technique [0002] High blood pressure greatly increases the risk of heart, brain, kidney and other organs, and also greatly increases the risk of other diseases. Because hypertensive patients can remain asymptomatic for many years, the World Health Organization calls hypertension a "stealth killer" of human health. According to statistics, there are 1.13 billion people in the world suffering from high blood pressure, of which less than one-fifth of the hypertensive patients can control their condition, among which the number of deaths due to cardiovascular disease (CVD) accounted for the largest number of deaths in the world in 2016 31% of the population and is one of the leading causes of death worldwide. Among them, high blood pressure is also one of the main factors leading to patients suffering fr...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P9/12A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P9/12C07K14/43504A23V2200/326
Inventor 孙坤来蔡诗颖高敏孟乐聂鹏王斌陈荫赵玉勤王玉梅
Owner 舟山市和朴海洋生物科技有限公司
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