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Preparation method of kelp micromolecular ACE inhibitory peptides with Tyr (tyrosine) at C tail end

A small molecule, inhibitory peptide technology, applied in the field of biotransformation to prepare small molecule ACE inhibitory peptides with tyrosine (Tyr) as the C-terminal, to achieve the effects of easy absorption, significant blood pressure lowering effect, and high ACE inhibitory activity

Inactive Publication Date: 2017-04-26
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the preparation process of mixed enzymatic hydrolysis conversion of small molecule antihypertensive active peptides containing Tyr at the C-terminus of seaweed food sources at home and abroad.

Method used

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  • Preparation method of kelp micromolecular ACE inhibitory peptides with Tyr (tyrosine) at C tail end
  • Preparation method of kelp micromolecular ACE inhibitory peptides with Tyr (tyrosine) at C tail end
  • Preparation method of kelp micromolecular ACE inhibitory peptides with Tyr (tyrosine) at C tail end

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for biotransformation of kelp crude protein to prepare a small molecule ACE inhibitory peptide with tyrosine (Tyr) as the C-terminus, characterized in that it comprises the following steps:

[0031] (1) Raw material pretreatment:

[0032] Wash fresh kelp or kelp processing waste to remove sediment and impurities, degrease with ether, extract with alcohol to remove mannitol, and precipitate with alcohol to remove polysaccharide macromolecules, dry at 60°C to constant weight, crush and pass through a 200-mesh sieve, and set aside;

[0033] (2) Preparation of kelp crude protein:

[0034] Take the kelp powder prepared in step (1), dissolve it with a buffer solution with a pH value of 9.5 at a solid-to-liquid volume ratio of 1:50, stir and extract at a constant speed at 45°C for 5 h, and dissolve the kelp protein under alkaline conditions. After the extraction, Use 1 mol / L hydrochloric acid to adjust the pH to 4.4 to precipitate laminarin, centrifuge at 12000 r / min...

Embodiment 2

[0046] A method for biotransformation of kelp crude protein to prepare a small molecule ACE inhibitory peptide with tyrosine (Tyr) as the C-terminus, characterized in that it comprises the following steps:

[0047] (1) Raw material pretreatment:

[0048] Wash fresh kelp or kelp processing waste to remove sediment and impurities, degrease with ether, extract with alcohol to remove mannitol, and precipitate with alcohol to remove polysaccharide macromolecules, dry at 65°C to constant weight, crush and pass through a 100-mesh sieve, and set aside;

[0049] (2) Preparation of kelp crude protein:

[0050] Take the kelp powder prepared in step (1), dissolve it with a buffer solution with a pH value of 9.0 at a solid-to-liquid volume ratio of 1:45, stir and extract at a constant speed at 45°C for 6 h, and dissolve the kelp protein under alkaline conditions. After the extraction, Use 1 mol / L hydrochloric acid to adjust the pH to 4.4 to precipitate laminarin, centrifuge at 12000 r / min...

Embodiment 3

[0060] A method for biotransformation of kelp crude protein to prepare a small molecule ACE inhibitory peptide with tyrosine (Tyr) as the C-terminus, characterized in that it comprises the following steps:

[0061] (1) Raw material pretreatment:

[0062] Wash fresh kelp or kelp processing waste to remove sediment and impurities, degrease with ether, extract with alcohol to remove mannitol, and precipitate with alcohol to remove polysaccharide macromolecules, dry at 60°C to constant weight, crush and pass through a 200-mesh sieve, and set aside;

[0063] (2) Preparation of kelp crude protein:

[0064] Take the kelp powder prepared in step (1), dissolve it with a buffer solution with a pH value of 9.0 at a solid-to-liquid volume ratio of 1:50, stir and extract at a constant speed at 45°C for 6 h, and dissolve the kelp protein under alkaline conditions. After the extraction, Use 1 mol / L hydrochloric acid to adjust the pH to 4.4 to precipitate laminarin, centrifuge at 12000 r / min...

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Abstract

The invention aims at providing a method for preparing micromolecular ACE (Angiotensin Converting Enzyme) inhibitory peptides with Tyr (tyrosine) at the C tail end by using seaweed crude protein as raw materials through mixed protease biotransformation. The method is simple; the implementation is easy; the preparation efficiency is high; the expanded production is facilitated; and a feasible scheme is provided for the development of a seaweed antihypertensive product. The high-ACE-inhibiting-activity micromolecular antihypertensive peptides with Tyr at the C tail end are prepared; the antihypertensive effect is obvious; and the prepared micromolecular antihypertensive peptides are white powder, can be easily dissolved in water and easily absorbed, has the advantages of small toxic and side effects and high safety, and can be used as an efficient raw material of antihypertensive prevention and treatment medicine.

Description

technical field [0001] The invention belongs to the field of preparation of plant angiotensin-converting enzyme (ACE) inhibitory oligopeptides, in particular to a method for biotransformation of crude kelp protein as a raw material to prepare small molecule ACE inhibitory peptides with tyrosine (Tyr) as the C-terminus. Background technique [0002] The production and processing of seaweed such as kelp in our country are huge. However, the processing of seaweed such as kelp is mainly carried out by small and medium-sized enterprises, the processing equipment and production technology are relatively backward, the product variety is small, and the quality is uneven. Low, the output value is not optimistic. According to the data, the by-products of seaweed processing such as kelp contain 5% to 20% of protein, and these by-products are directly discarded without processing, resulting in a great waste of resources; if waste can be turned into treasure, it can be deeply developed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K5/068C07K5/083C07K5/103C07K7/06C07K5/11C07K5/065
CPCC12P21/06C07K5/06078C07K5/06095C07K5/0806C07K5/1013C07K5/1019C07K7/06
Inventor 陈继承王洁臧盈盈葛慧芳
Owner FUJIAN AGRI & FORESTRY UNIV
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