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Solid composite microbial agent for soil remediation and preparation method of solid composite microbial agent

A compound microbial agent and soil remediation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor and single soil remediation ability, and achieve the effect of improving soil remediation ability

Pending Publication Date: 2021-02-23
阳田生物科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of this application is to provide a kind of solid compound microbial bacterial agent and its preparation method for soil remediation, to solve the combination of more different fungi, actinomycetes and subtilis bacteria that are not widely combined in the prior art, more It is a single strain, or a combination of several subtilis bacteria, which leads to the problem of poor soil remediation ability

Method used

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  • Solid composite microbial agent for soil remediation and preparation method of solid composite microbial agent
  • Solid composite microbial agent for soil remediation and preparation method of solid composite microbial agent
  • Solid composite microbial agent for soil remediation and preparation method of solid composite microbial agent

Examples

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Effect test

Embodiment 1

[0028] A solid composite microbial agent for soil remediation, comprising the following components in weight percentage: 35%-45% of actinomycetes, 3%-7% of Bacillus subtilis, and 3%-7% of mucormycetes , Rhizopus content is 3%-7%, head mold content is 3%-7%, Hansenula content is 3%-7%, ascomycete content is 3%-7%, niger content is 5% -15%, Penicillium 5%-15% and Neurospora tetrasporum 5%-15%.

[0029] Among them, actinomycetes refer to terrestrial and powerful prokaryotes that mainly grow in hyphae and reproduce with spores, and have the functions of weeding and anti-parasite;

[0030] Bacillus subtilis spore exists in the soil remediation agent with the form of dormant state, and the bacterium of spore form has the characteristics of high temperature resistance, salt resistance, and drying resistance; Rapid growth and reproduction under suitable water and temperature, the extracellular enzymes produced by it can decompose the organic matter in the soil and directly provide nu...

Embodiment 2

[0054] A solid compound microbial agent for soil remediation, comprising: 40% actinomycetes, 5% bacillus subtilis, 5% mucormycetes, 5% rhizopus and 5% head mold , Hansenula content is 5%, Ascomycetes content is 5%, Aspergillus niger content is 10%, Penicillium content is 10% and Neurospora tetrasporum content is 10%.

[0055] Among them, actinomycetes refer to terrestrial and powerful prokaryotes that mainly grow in hyphae and reproduce with spores, and have the functions of weeding and anti-parasite;

[0056] Bacillus subtilis spore exists in the soil remediation agent with the form of dormant state, and the bacterium of spore form has the characteristics of high temperature resistance, salt resistance, and drying resistance; Rapid growth and reproduction under suitable water and temperature, the extracellular enzymes produced by it can decompose the organic matter in the soil and directly provide nutrients to the crops, and its metabolites can stimulate metabolites, which ca...

Embodiment 3

[0063] Embodiment 3: preparation method comprises the following steps:

[0064] Step 1) The liquid medium is composed of phosphoric acid, manganese sulfate, ammonium sulfate, and then a mixture of mint extract, asparagine, protein, and glycerin. In a stainless steel sterile liquid fermentation tank, the temperature is controlled at 30-37 degrees, The PH value is controlled at 4-6 and cultivated for 24 hours;

[0065] Step 2) Before adding solid fermentation, all materials must be sterilized first to ensure that they are sterile and then added to a solid sterile stainless steel fermenter. The solid fermentation product uses vermiculite as the carrier and molasses as the main carbon source. Soybean powder, yeast powder, oyster shell powder, chitosan;

[0066] Step 3) After 24 hours of liquid culture, take a sample to detect the concentration of the bacteria, and after confirming the growth of the bacteria, transfer the bacteria liquid into a sterile stainless steel fermenter fo...

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Abstract

The invention discloses a solid composite microbial agent for soil remediation and a preparation method of the solid composite microbial agent. The microbial agent comprises the following components in percentage by weight: 35%-45% of actinomycetes, 3%-7% of bacillus subtilis, 3%-7% of mucor, 3%-7% of rhizopus, 3%-7% of cephalomycetes, 3%-7% of hansenula polymorpha yeast, 3%-7% of ascomycetes, 5%-15% of aspergillus niger, 5%-15% of penicillium and 5%-15% of neurospora tetrasperma. The technical problem that in the prior art, more different fungi, actinomycetes and bacillus subtilis are not widely combined, a single strain is more used, or several kinds of bacillus subtilis are compounded, and consequently the soil remediation capacity is poor is solved.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a solid composite microbial agent for soil restoration and a preparation method thereof. Background technique [0002] In the agricultural farming environment, due to the long-term use of various chemical agents, such as chemical fertilizers, herbicides, insecticides and fungicides, etc., the microbial balance of the soil is destroyed, which in turn leads to a decrease in the absorption and utilization of plant fertilizers, soil acidification, soil Issues such as pests and diseases. [0003] In view of the previous technologies in related technologies, there is no extensive combination of more different fungi, actinomycetes and subtilis, more of a single strain, or a combination of several subtilis, resulting in poor soil remediation capabilities problem, no effective solution has yet been proposed. Contents of the invention [0004] The main purpose of this application ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14B09C1/10C12R1/04C12R1/125C12R1/785C12R1/845C12R1/78C12R1/685C12R1/80C12R1/645
CPCC12N1/20C12N1/16C12N1/14B09C1/10B09C1/105
Inventor 周廷霖
Owner 阳田生物科技(江苏)有限公司
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