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A nucleic acid extraction and amplification test tube and its working method

An extraction tube and test tube technology, applied in the field of nucleic acid extraction and amplification test tubes, can solve the problems of cumbersome operation, inability to meet the needs of SARS-CoV2 detection, cumbersome and other problems

Active Publication Date: 2021-07-20
SUZHOU MOLARRAY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Automatic control, only need to add samples manually in the whole operation process; however, there are as many as 17 kinds of reagents, and the operation requires suction and rotation, which is cumbersome; moreover, it requires magnetic separation
[0013] It can be seen that in the prior art, most of the research still stays on how to extract nucleic acid; while a small amount of research on the integrated function of nucleic acid extraction and amplification has problems of cumbersome operation and complicated equipment design, which cannot meet the requirements of SARS- The need for CoV2 testing

Method used

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  • A nucleic acid extraction and amplification test tube and its working method
  • A nucleic acid extraction and amplification test tube and its working method
  • A nucleic acid extraction and amplification test tube and its working method

Examples

Experimental program
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Embodiment 1

[0101] Embodiment one, refer to figure 1 As shown, a nucleic acid extraction and amplification test tube includes: PCR reaction tube 1, extraction tube assembly lower tube 2, extraction tube assembly middle tube 3, extraction tube assembly upper tube assembly 4, top cover 5, piston rod 6, the second A spring 7, a piston ring 8, a first spool 9, a second spring 10, a second spool 11, and a third spring 12;

[0102] Wherein, the top of the PCR reaction tube 1 is provided with an opening, and the top opening of the PCR reaction tube 1 is connected to the bottom of the lower tube 2 of the extraction tube assembly;

[0103] The upper pipe assembly 4 of the extraction pipe assembly, the middle pipe 3 of the extraction pipe assembly, and the lower pipe 2 of the extraction pipe assembly are sequentially connected into one body from top to bottom; a top cover 5 is connected to the top of the extraction pipe of the upper pipe assembly 4 of the extraction pipe assembly;

[0104] At the...

Embodiment 2

[0155] Embodiment 2, the purpose of embodiment 2 is to realize fully automatic process from sample collection to qPCR result output;

[0156] One-step POCT instant nucleic acid detection device, including: PCR instrument 101, extraction tube fixing plate 102, piston rod fixing plate 103, two motors 104, coupling 105, bearing housing 106, two circular guide rails 107, ball screw 108, screw nut 109, linear bearing 110, PCR reaction tube fixing seat;

[0157] A plurality of PCR reaction tube fixing seats are arranged on the upper surface of the PCR instrument 101, and the PCR reaction tube fixing seats are adapted to the shape of the PCR reaction tube;

[0158] The output shafts of the two motors are connected to the coupling 105, and the coupling 105 is connected to the ball screw 108;

[0159] Two circular guide rails 107 are arranged in parallel and the axis direction is vertical;

[0160] The extraction tube fixing plate 102 and the piston rod fixing plate 103 are arranged in...

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Abstract

The invention discloses a nucleic acid extraction and amplification test tube and its working method, which belong to the field of molecular diagnosis and detection instruments, and its design points include: a PCR reaction tube, a lower tube of an extraction tube assembly, a middle tube of an extraction tube assembly, and an extraction tube assembly Upper tube assembly, top cover, piston rod, piston ring, first spool, second spring, second spool, third spring; extraction tube assembly upper tube assembly, extraction tube assembly middle tube, extraction tube assembly lower tube, The PCR reaction tubes are connected sequentially from top to bottom; the extraction tube assembly The upper tube assembly includes: two vertical parallel tube bodies: an extraction tube and a piston tube; a top cover is arranged on the top of the extraction tube; the piston rod is placed in the piston tube. The invention aims to provide a nucleic acid extraction and amplification test tube and its working method, which greatly improves the detection efficiency.

Description

technical field [0001] The present invention relates to the field of a medical inspection instrument and a molecular diagnosis instrument, and more specifically relates to a nucleic acid extraction and amplification test tube and its working method. Background technique [0002] Current methods for detecting and diagnosing microbial infections involve the use of quantitative polymerase chain reaction (qPCR) to amplify nucleic acid (RNA / DNA) in patient samples to confirm the presence of pathogens. Additionally, these tests must be done by a testing laboratory where two steps must be performed for diagnosis: nucleic acid extraction followed by qPCR. These two procedures may also be performed by different devices, adding to the complexity and length of time required between sample collection and diagnosis. Finally, these laboratory tests are often done manually, involving physical labor and potentially additional hazards when handling pathogen-positive samples. [0003] Curre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/24C12M1/04C12M1/00
CPCB01L7/52
Inventor 王威梅宗羲聂晶
Owner SUZHOU MOLARRAY CO LTD
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