Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Heme oxygenase gene and effect of heme oxygenase gene in regulation and control of ganoderma lucidum polysaccharide biosynthesis

A Ganoderma lucidum polysaccharide and biosynthesis technology, applied in the field of molecular biology, can solve the problems of lagging microbial research

Active Publication Date: 2021-02-05
NANJING AGRICULTURAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on animal and plant heme oxygenase is relatively clear and clear, while the research on microorganisms is relatively lagging behind

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Heme oxygenase gene and effect of heme oxygenase gene in regulation and control of ganoderma lucidum polysaccharide biosynthesis
  • Heme oxygenase gene and effect of heme oxygenase gene in regulation and control of ganoderma lucidum polysaccharide biosynthesis
  • Heme oxygenase gene and effect of heme oxygenase gene in regulation and control of ganoderma lucidum polysaccharide biosynthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Construction of Ganoderma lucidum HMX1 Silenced Strain

[0031] 1. Ganoderma lucidum HMX1 sequence analysis

[0032] In the present invention, the amino acid sequence of HMX1 protein (protein number: GAW02255.1) obtained by searching in NCBI (https: / / www.ncbi.nlm.nih.gov / ). The sequence was compared with the known Ganoderma lucidum protein library to obtain a possible HMX1 protein (protein number MN388942) in Ganoderma lucidum, and the corresponding sequence was obtained in the Ganoderma lucidum cDNA library according to the protein number. HMX1 contains 1092 bases (as shown in SEQ ID NO.1) and encodes 393 amino acids (as shown in SEQ ID NO.2). Multiple sequence alignment results showed that Ganoderma lucidum HMX1 protein contained the classic Heme_oxygenase complex domain ( figure 1 ), indicating that the protein we found may be Ganoderma lucidum HMX1 protein. In addition, by comparing the genomic DNA and cDNA sequences of Ganoderma lucidum HMX1, we found...

Embodiment 2

[0050] Example 2: Effects of Heme Oxygenase Gene HMX1 Silencing on Ganoderma Lucidum Heme Oxygenase Activity and Carbon Monoxide Content

[0051] Heme oxygenase has been studied clearly in animals and plants. It is an inducible protein, and its expression is easily induced by external stimuli. It is mostly reported to have anti-oxidation and cell protection effects. Carbon monoxide (CO), an important product of heme oxygenase, is a gas signal molecule that can respond to external stimuli in various ways.

[0052] In this patent, we detected the heme oxygenase activity and CO content in the HMX1 silenced strain.

[0053] The specific detection method of heme oxygenase enzyme activity is as follows:

[0054] (1) We took the mycelia grown on the CYM plate for 7 days, ground them into powder with liquid nitrogen, and then dissolved them in a solution containing 250mmol / L mannitol, 10% (v / v) glycerol, 1% (w / v) poly Vinylpyrrolidone, 1mmol / L EDTA, 1mmol / L dithiothreitol in 25mmol / ...

Embodiment 3

[0064] Example 3: Effects of silencing of the heme oxygenase gene HMX1 on the biosynthesis of Ganoderma lucidum polysaccharides

[0065] Important role of Ganoderma lucidum HMX1 in polysaccharide biosynthesis of Ganoderma lucidum

[0066] In this patent, we detected the exopolysaccharide content and intracellular polysaccharide content of Ganoderma lucidum in HMX1 silencing strains.

[0067] The method for determining the content of exopolysaccharide by phenol-sulfuric acid method is as follows:

[0068] (1) Select the outermost mycelium block of mycelial growth in the PDA plate and inoculate it in liquid PDA, culture at 150 rpm and 28° C. for 7 days. The prepared liquid strains were aseptically crushed with a homogenizer, inoculated in CYM liquid medium at an inoculum size of 5% (v / v), and fermented at 150 rpm at 28° C. for 7 days.

[0069] (2) Take 0.5 mL of the fermented culture filtrate and mix it with 4 times the volume of 95% ethanol, shake vigorously, precipitate with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of gene engineering, and particularly discloses a heme oxygenase gene and an effect of the heme oxygenase gene in regulation and control of ganoderma lucidum polysaccharide biosynthesis. According to the invention, a ganoderma lucidum HMX1 gene is silenced through a gene silencing technology, so that ganoderma lucidum polysaccharide biosynthesis amountcan be increased. Besides, an RNA interference means is adopted so as to provide an important genetic manipulation means for improving the biosynthesis of the ganoderan by silencing the heme oxygenasegene; and thus, a theoretical basis is provided for improving the ganoderma lucidum polysaccharide biosynthesis by using a genetic engineering technical means.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to a heme oxygenase gene (heme oxygenase, HMX1) and its application in regulating the biosynthesis of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is a medicinal fungus that is widely grown and used in East Asia. It has anti-inflammatory and antioxidant properties and is beneficial to health when used correctly. With the deepening of research, Ganoderma lucidum has been found to have various effects, including anti-inflammatory, anti-oxidant, anti-glycemic, anti-ulcer, anti-cancer and immune stimulation. Ganoderma lucidum contains a variety of active ingredients, including triterpenes, polysaccharides, nucleotides, sterols, steroids, etc., among which polysaccharides have multiple effects and are believed to have anti-tumor effects through anti-angiogenesis and immune regulation. In addition, polysaccharides have protective effects a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/80C12P19/04C12R1/645
CPCC12N9/0083C12Y114/99003C12N15/80C12P19/04
Inventor 任昂赵明文于汉寿王婷师亮朱静刘锐姜爱良
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com