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A method of using supramolecular inclusion agent to close the antibacterial agent activity in collagen solution

A supramolecular, antibacterial technology, applied in the field of antibacterial activity, can solve problems such as nerve damage, damage to the inherent biocompatibility of collagen, and toxicity

Active Publication Date: 2021-09-21
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional antibacterial and antifungal agents have low bioselectivity, not only can kill harmful microorganisms such as bacteria, but are also toxic to eukaryotic cells, which may cause adverse reactions such as allergies, inflammation, and even nerve damage, and damage the inherent biocompatibility of collagen

Method used

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  • A method of using supramolecular inclusion agent to close the antibacterial agent activity in collagen solution
  • A method of using supramolecular inclusion agent to close the antibacterial agent activity in collagen solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Disperse 2 parts of ciprofloxacin in 40 parts of dichloromethane, then add 50 parts of distilled water dissolved in 3 parts of potassium carbonate into it, and stir evenly to obtain component 1; at the same time, 8 parts of bromoacetyl bromide Dissolved in 20 parts of dichloromethane, slowly added to component 1 at 0°C within 1.5 hours, continued to stir for 2 hours, then raised to room temperature and stirred for 13 hours; finally, the product was washed with 5% hydrochloric acid aqueous solution and distilled water successively After washing, the organic layer was taken, and vacuum-dried to constant weight to obtain the first-step synthetic product;

[0023] (2) Dissolve 2 parts of the product synthesized in the first step in 30 parts of dichloromethane, then add 3 parts of amantadine to the above solution, and continue stirring at 50°C for 13 hours. The obtained product is vacuum-dried to constant weight to obtain the target product product;

[0024] (3) Add 0.2...

Embodiment 2

[0028] (1) Disperse 1 part of ciprofloxacin in 30 parts of toluene, then add 40 parts of distilled water dissolved in 2 parts of sodium carbonate into it, stir well to obtain component 1; at the same time, dissolve 5 parts of bromoacetyl bromide in 10 parts of toluene, at 2°C, slowly added to component 1 within 1 hour, continued to stir for 1 hour, then raised to room temperature and stirred for 12 hours; finally, the product was washed successively with 5% sulfuric acid aqueous solution and distilled water, and the organic layer, and vacuum-dried to constant weight to obtain the first-step synthesis product;

[0029] (2) Dissolve 1 part of the product synthesized in the first step in 10 parts of toluene, then add 2 parts of amantadine to the above solution, continue stirring at 40°C for 12 hours, and vacuum-dry the obtained product to constant weight to obtain the target product;

[0030] (3) Add 0.1 part of the above-mentioned target product to 100 parts of collagen solution...

Embodiment 3

[0034] (1) Disperse 4 parts of ciprofloxacin in 60 parts of n-butanol, then add 70 parts of distilled water dissolved in 6 parts of calcium bicarbonate into it, and stir evenly to obtain component 1; at the same time, 13 parts of bromoacetyl chloride Parts were dissolved in 40 parts of n-butanol, at 4 ° C, slowly added to component 1 within 2 hours, continued to stir for 4 hours, and then raised to room temperature and stirred for 14 hours; finally, the product was washed with 10% aqueous nitric acid and distilled water Washing in sequence, taking the organic layer, and vacuum drying to constant weight to obtain the first-step synthesis product;

[0035] (2) Dissolve 3 parts of the product synthesized in the first step in 40 parts of n-butanol, then add 4 parts of amantadine to the above solution, and continue stirring at 60°C for 14 hours. The obtained product is vacuum-dried to constant weight to obtain the target product;

[0036] (3) Add 0.4 parts of the above target prod...

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Abstract

The invention discloses a method for closing the activity of antibacterial agents in collagen solution by using supramolecular inclusion agent. In this method, amantadine is coupled to the secondary amine group on the piperazine ring of ciprofloxacin, and the specific supramolecular inclusion between cucurbit[7]uril and its derivatives and amantadine is used to prevent ciprofloxacin The star is transported across the outer membrane through the OmpF channel of the outer membrane porin of Gram-negative bacteria to realize the on-demand shutdown of the antibacterial agent activity contained in the collagen solution, so as to coordinate the storage stability and use safety of the collagen solution, and solve the anticorrosion necessity of the natural collagen antibacterial solution The long-standing contradiction between the stability and the maintenance of biocompatibility integrity lays the foundation for breaking through the application limitations of medical collagen solutions.

Description

technical field [0001] The invention relates to a method for closing the activity of an antibacterial agent in a collagen solution by using a supramolecular inclusion agent, and belongs to the field of biomass materials. Background technique [0002] Collagen is a renewable resource with a unique structure and diverse functions. Compared with other artificial materials, collagen has excellent biocompatibility. After being made into a solution, it is widely used in clinical scenarios such as injection wrinkle removal and ophthalmic treatment. However, natural collagen is rich in nutrients and is a good carrier for microbial growth, but it has the disadvantage of being prone to mold and long bacteria. Therefore, antibacterial and antisepsis is a necessary prerequisite for the high-value utilization of collagen solutions in the biomedical field. [0003] In order to prevent the collagen solution from becoming moldy and growing bacteria, the most commonly used method is to add ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K31/496A61K47/42A61P31/04A61P27/02A61K8/49A61K8/65A61Q19/08
CPCA61K8/494A61K8/4953A61K8/65A61K31/496A61K47/42A61K2800/91A61Q19/08A61K47/6949A61P27/02A61P31/04
Inventor 陈意孙莎莎杨高夫颜俊王忠辉曾琦王睿
Owner SICHUAN UNIV
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