Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of nano vaccine with pH and reduction dual sensitivity and obtained product

A nano-vaccine, a sensitive technology, is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. It can solve the problems of antigen dissociation, toxic and harmful tissue cells, etc. , the effect of improving efficacy and biosafety

Inactive Publication Date: 2021-02-05
HAINAN UNIVERSITY
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, exposed PEI is toxic and harmful to normal tissue cells to some extent, and the loaded antigen may be prone to dissociation from MSNs in interstitial fluid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of nano vaccine with pH and reduction dual sensitivity and obtained product
  • Preparation method of nano vaccine with pH and reduction dual sensitivity and obtained product
  • Preparation method of nano vaccine with pH and reduction dual sensitivity and obtained product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation process of nano-vaccine with pH and reduction sensitivity is as follows: figure 1 As shown, the specific steps are as follows:

[0041]Synthesis of PEI-modified mesoporous silica nanospheres (PMSNs): 20 mg of lyophilized MSNs were dispersed in 10 mL of ultrapure water by sonication. Add PEI solution (2 mL, 20 mg / mL) to the above MSN suspension. After magnetic stirring for 2 hours, the PEI-modified mesoporous silica nanospheres were collected by centrifugation (12000 rpm, 15 min), and washed 3 times with pure water to remove free PEI. Finally, pure PEI-modified mesoporous silica nanospheres were obtained by lyophilization, denoted as PMSN.

[0042] Loading of ovalbumin on PMSN: Suspend PMSN (1 mg) in 0.5 mL of pure water, and then suspend 0.5 mL of 2.0 mg / mL ovalbumin (OVA) solution in the suspension. The mixture was shaken rapidly at room temperature for 1 hour and then centrifuged (12000 rpm, 15 min). The solid at the bottom was washed three times w...

Embodiment 2

[0047] Synthesis of PEI-modified mesoporous silica nanospheres (PMSNs): 18 mg of lyophilized MSNs were dispersed in 10 mL of ultrapure water by sonication. PEI solution (2 mL, 20 mg / mL) was added to the above MSN suspension. After magnetic stirring for 2.5 h, the PEI-modified nanoparticles were collected by centrifugation (12000 rpm, 15 min), and washed 3 times with pure water to remove free PEI. Finally, pure PEI-modified mesoporous silica nanospheres were obtained by lyophilization, denoted as PMSN.

[0048] Loading of ovalbumin on PMSN: Suspend PMSN (1 mg) in 0.5 mL of pure water, and then suspend 0.5 mL of 1.6 mg / mL ovalbumin (OVA) solution in the suspension. The mixture was shaken rapidly at room temperature for 1 h, and then centrifuged (12000 rpm, 15 min). The bottom solid after centrifugation was washed three times with pure water to remove free OVA to obtain PMSN@OVA.

[0049] Synthesis of catechol-terminated eight-armed PEG (CPEG): 4-(2-aminoethyl)-1,2-benzenediol...

Embodiment 3

[0053] Study on OVA release behavior of PMSN@OVA-MPN in vitro

[0054] In order to study the OVA release behavior of PMSN@OVA-MPN in different media, take an appropriate amount of PMSN@OVA-MPN prepared in Example 1 (in which OVA is marked with FITC), divide it into three parts on average, and disperse them in A : 4 mL PBS (pH=7.4), B: 4 mL PBS (pH=7.4), and C: 4 mL PBS (pH=5) in three portions of PBS and stirred at 37°C. At the 7th hour of stirring, reduced glutathione was added to one neutral PBS suspension at pH = 7.4 to reach a concentration of 10 mM, but another neutral PBS and weakly acidic PBS suspension was maintained throughout No glutathione was added in the process. Centrifuge at different time points (12000 rpm, 5 min), then extract 800 μL supernatant from these suspensions for detection, and supplement the remaining suspension with corresponding 800 μL glutathione solution (10 mM), neutral PBS or weakly acidic PBS, and then continue to stir. Among them, add glut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a nano vaccine with pH and reduction double sensitivity and an obtained product. The nano vaccine is obtained by taking PEI-modified mesoporous silica nanospheres as a raw material, adsorbing a model antigen through electrostatic interaction, and then wrapping an outer layer with a metal phenolic network with reduction sensitive disulfide bonds as aprotection network. The preparation method is simple and convenient to operate, low in energy consumption, environment-friendly and easy to expand production, and the obtained nano vaccine has very good in-vivo long-term stability and biocompatibility and has remarkable treatment and prevention effects on tumors.

Description

technical field [0001] The invention relates to a preparation method of a nano-vaccine with dual sensitivity to pH and reduction and the resulting product, belonging to the technical field of nano-material preparation. Background technique [0002] Among various nanomaterials used as carriers for delivering tumor-specific antigens, mesoporous silica nanoparticles (MSNs) are expected to be the first choice due to their high loading capacity, easy surface property improvement and good biocompatibility. However, due to the low efficiency of antigen cross-presentation, general MSNs cannot meet the requirements for eliciting effective anticancer immune responses. With this in mind, MSNs could be modified with polyethyleneimine (PEI), which promotes lysosomal escape through the proton sponge effect and thereby induces strong cellular immunity, to improve the adjuvant effect of nanocarriers. However, exposed PEI is toxic and harmful to normal tissue cells to a certain extent, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/10A61K47/69A61K47/58A61K47/52A61K39/00A61P35/00
CPCA61K9/5146A61K47/6925A61K47/58A61K47/52A61K39/0011A61P35/00A61K2039/575A61K2039/572A61K2039/585
Inventor 杨永苏乾洪符钊铭马荣英
Owner HAINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com