Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting in-vivo psychotropic drugs and metabolites thereof, and application thereof

A technology for metabolites and drugs, applied in the field of blood drug concentration detection, can solve the problems of increasing analytical throughput, sensitivity, chromatographic peak capacity, and expensive equipment

Active Publication Date: 2021-02-02
SHANGHAI MENTAL HEALTH CENT (SHANGHAI PSYCHOLOGICAL COUNSELLING TRAINING CENT)
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mass spectrometry can avoid the problem of quantitative interference, due to the use of mass spectrometry, not only the equipment is more expensive, but ultra performance liquid chromatography (Ultra Performance Liquid Chromatography, UPLC) covers small New technologies such as particle packing, very low system volume and rapid detection methods increase the throughput, sensitivity and chromatographic peak capacity of the analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting in-vivo psychotropic drugs and metabolites thereof, and application thereof
  • Method for detecting in-vivo psychotropic drugs and metabolites thereof, and application thereof
  • Method for detecting in-vivo psychotropic drugs and metabolites thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: complete detection method and standard solution sample injection

[0042] A method for detecting psychotropic drugs and metabolites thereof in blood or hair, said method adopting ultra-high performance liquid chromatography-ultraviolet detection method (i.e. UPLC-UV method), comprising the following steps:

[0043] S1 chromatographic conditions: Chromatographic column: C18 chromatographic column, ACQUITY UPLC BEH C18 diameter 2.1mm*length 50mm, its filler particle size is 1.7μm; mobile phase: A phase is 0.05% v / v formic acid-water solution, B phase is 0.05% v / v formic acid-methanol solution; detector is ultraviolet (UV) DAD detector, UVλ max : Full wavelength scanning; Column temperature: 40°C; Mobile phase flow rate: 0.2mL / min; Injection volume: 10.0μL;

[0044] The preparation of S2 working solution includes:

[0045] S20: Preparation of needle washing solution: Measure 700mL of water into the liquid phase washing bottle with a graduated cylinder, then...

Embodiment 2

[0051] Embodiment 2 sample serum: patient's serum chromatogram of taking clozapine medicine

[0052] The preparation method of S1 chromatographic condition, S21 internal standard solution, S22 mixed standard solution is the same as embodiment 1;

[0053] Preparation of S231 serum test solution: S2311: Take 20 μL of the internal standard solution prepared in the above step S21 and dry it with nitrogen gas, mix it with 500 μL of thawed frozen serum sample to be tested into a 7 mL centrifuge tube, vortex for 1 min for the first time, and add 200 μL Aqueous sodium hydroxide solution with a concentration of 2mol / L, then add 3mL of methyl tert-butyl ether, and then vortex for 3min for the second time; The final supernatant was blown dry with nitrogen gas, then redissolved in 80 μL 50% v / v methanol aqueous solution, vortexed for 1 min, and the product was obtained;

[0054] S3 assay method: need testing solution is injected ultra-high performance liquid chromatograph, adopts gradien...

Embodiment 3

[0055] Embodiment 3 sample serum: the serum chromatogram of the patient who takes paliperidone (9-OH risperidone)

[0056] The preparation method of S1 chromatographic condition, S21 internal standard solution, S22 mixed standard solution is the same as embodiment 1;

[0057] S231 need testing solution preparation, S3 measuring method are with embodiment 2: as image 3 As shown, obtain the retention time of the psychotropic drug and its metabolites (t R ), that is; the retention time t of paliperidone R 14.514min, carbamazepine 27.341min (internal standard).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a method for simultaneously detecting mental disease drugs and metabolites thereof in human blood or hair by adopting an ultra-high performance liquid chromatography ultravioletdetection method. Chromatographic conditions are as follows: (1) a chromatographic column which adopts a C18 chromatographic column; (2) a mobile phase comprising a phase A which is a 0.05 v / v% formic acid aqueous solution, and a phase B which is a 0.05 v / v% formic acid-methanol solution; and (3) a detector which is an ultraviolet detector; Separation and analysis of common mental disease drugs and metabolites thereof are realized through a gradient elution program. The separation and analysis method adopted by the invention is simple, does not use expensive mass spectrum, and has significantacademic significance and economic value for guiding clinical medication and analyzing samples.

Description

technical field [0001] The invention relates to the detection field of blood drug concentration, in particular to a method and application for detecting psychotropic drugs and their metabolites in vivo. Background technique [0002] Antipsychotic drugs are also called strong tranquilizers or neuroleptics. A group of drugs used to treat schizophrenia and other psychotic disorders. The usual therapeutic dose does not affect the patient's intelligence and consciousness, but it can effectively control the patient's psychomotor excitement, hallucinations, delusions, hostility, thinking disorders and abnormal behaviors and other mental symptoms. Antidepressive drugs refer to a group of psychiatric drugs mainly used to treat mental diseases with emotional depression as the prominent symptom. The difference from stimulants is that it can only eliminate the depressive symptoms of depressed patients, but cannot improve the mood of normal people. [0003] Therapeutic drug concentrat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813G01N2030/8822
Inventor 孙秀佳张晨李春波王丽华陈艳刘红梅蒋平任娟娟
Owner SHANGHAI MENTAL HEALTH CENT (SHANGHAI PSYCHOLOGICAL COUNSELLING TRAINING CENT)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com