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Method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds

A technology of compounds and phenolic glycosides, which is applied in the field of plant extraction and separation, to achieve the effects of large extraction volume, simple extraction process, and recyclable solvents

Active Publication Date: 2021-02-02
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no report on the isolation and extraction of phenolic glycosides (such as 3,5-dihydroxyphenethyl alcohol-3-O-β-pyranoside, broadbactin C, etc.) from Nigella sativa seeds

Method used

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  • Method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds
  • Method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds
  • Method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds

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Embodiment 1

[0031] A method for separating and extracting phenolic glycosides (3,5 dihydroxyphenylethyl alcohol-3-O-beta-pyranoside, broadbactin C) from Nigella sativa seeds, comprising the following steps:

[0032] 1) Use 5kg of dry Nigella sativa seeds as raw material, soak and degrease with 7L petroleum ether at room temperature after crushing (defatting 3 times, each degreasing time is 3 days), evaporate the residue to dry petroleum ether, cool with 70% ethanol at room temperature Leach and extract 3 times (the ratio of residue to 70% ethanol to liquid is 5kg:7L), each time for 3 days, combine the extracts, recover the solvent under reduced pressure, and obtain the extract extract;

[0033] 2) Dissolve the extract extract in methanol, mix the sample on a silica gel column, and use dichloromethane- Gradient elution was performed on the silica gel column with methanol, and combined by silica gel thin-layer chromatography to obtain 7 components, which were labeled as Fr1-Fr7 according to...

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Abstract

The invention relates to a method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds. The method comprises the following steps that dry Nigella sativa seeds are pulverized, degreasing is carried out with petroleum ether, extracting is carried out with 60-80% ethanol, and a solvent is recycled under reduced pressure to obtain an extract; 2) the extract is dissolvedwith methanol, the extract is mixed with a silica gel column, and gradient elution is carried out with dichloromethane methanol to obtain seven components Fr1-Fr7; 3) silica gel column chromatographyseparation is performed on a fourth component Fr4 to obtain five components (4-1)-(4-5); and 4) gel column chromatography and silica gel column chromatography separation are performed on a component4-3 to obtain 3,5-dihydroxyphenylethanol-3-O-beta-pyranoside, and reversed-phase column chromatography and Sephadex LH-20 sephadex column chromatography separation are performed on a component 4-2 toobtain broadleaf chrysanthemum glycoside C. The method is simple in extraction process, a product is easy to obtain, the solvent can be recycled, the extraction amount is large, and industrial production can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and separation, in particular to a method for effectively and rapidly separating and extracting phenolic glycosides (such as 3,5-dihydroxyphenethyl alcohol-3-O-β-pyranoside, broad-spectrum Baccoside C, etc.) method. Background technique [0002] Nigella sativa seeds are the dried seeds of Nigella sativa, a plant of the Ranunculaceae Nigella genus. It is native to Southwest Asia and widely distributed in the Mediterranean, Central Europe, West Asia and other places; its seed oil is used as edible oil in the Middle East and North African countries such as It is widely used in Egypt, and at the same time, it is also used in health food to fight diseases, and it is widely used in food. The main components of Nigellasativa seeds are flavonoids, saponins, alkaloids, steroids and phenols, etc., and also contain a lot of oil. Nigella sativa seeds have a variety of pharmacological effects, inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 刘振花康文艺张岩马常阳牛云周莉陈思潭蒙丽君
Owner HENAN UNIVERSITY
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