Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Green colistin extraction method

A colistin and green technology, applied in the field of medicine, can solve the problems of high cost of sewage treatment, high environmental pollution, long process, etc., and achieve the effects of low cost of sewage treatment, short process and less waste liquid

Inactive Publication Date: 2021-01-22
HEBEI SHENGXUE DACHENG PHARMA
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems in the above-mentioned prior art, the present invention provides a green method for extracting colistin, which is characterized by simple, efficient, and zero-waste water extraction of colistin from fermentation broth, and solves the problem of long working procedures of other extraction methods. , high cost of sewage treatment and high pollution to the environment, it is an economical, green and environmentally friendly extraction method suitable for industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Green colistin extraction method
  • Green colistin extraction method
  • Green colistin extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]The potency of the fermentation broth is 645,000 u / mL, heated to 70℃, acidified to 1.8 with oxalic acid, stirred for 30 minutes, then added calcium hydroxide to adjust the pH value to 9.5, stirred for 30 minutes, precipitation occurs, filter with a plate and frame, and save the filtrate for next Batch fermentation water; add the solid filter cake to the sulfuric acid solution of 1 / 10 volume of the fermentation broth, stir to dissolve, and dissolve the pH value of 6.2; add activated carbon and purifying agent to the dissolving solution, filter through the plate and frame to obtain the colistin concentrate; concentrate After resin desalination, ultrafiltration to remove endotoxin, and finally spray-dried to obtain the finished product.

[0025]The filtrate is used as the next batch of fermentation water. The quality of the test is shown in Table 1:

[0026]

Embodiment 2

[0028]The filtrate in Example 1 was adjusted to pH 7.0 and used as fermentation water for fermentation. The pot had a potency of 647,000 u / mL. It was diluted with the ultrafiltration retentate in Example 1, heated to 45°C, and acidified by adding oxalic acid. To 1.6, stir for 30 minutes, then add calcium oxide to adjust the pH to 10.5, stir for 30 minutes, precipitation occurs, filter with a plate and frame, and reserve the filtrate as the next batch of fermentation water; add the solid filter cake to the sulfuric acid solution of 1 / 15 volume of the fermentation broth, Stir and dissolve to dissolve the pH value of 5.7; add activated carbon and purifying agent to the dissolving solution, and filter through plate and frame to obtain a colistin concentrate; the concentrate is desalinated by resin, ultrafiltration to remove endotoxin, and finally spray dried to obtain a finished product.

[0029]The filtrate is used as the next batch of fermentation water, and the tested quality is shown i...

Embodiment 3

[0032]The filtrate in Example 2 was adjusted to pH 6.0 and used as fermentation water for fermentation. The pot had a potency of 643,000 u / mL. It was diluted with the ultrafiltration retentate in Example 2, heated to 60°C, and acidified by adding phosphoric acid. To 2.0, stir for 30 minutes, then add calcium hydroxide to adjust the pH to 11.0, stir for 30 minutes, precipitation occurs, filter with a plate and frame, and reserve the filtrate as the next batch of fermentation water; add the solid filter cake to the sulfuric acid solution of 1 / 12 volume of the fermentation broth , Stir and dissolve to dissolve the pH value of 6.0; add activated carbon and purification agent to the dissolving solution, and filter through plate and frame to obtain a colistin concentrate; the concentrate is desalted by resin, ultrafiltration to remove endotoxin, and finally spray-dried to obtain the finished product.

[0033]The filtrate is used as the next batch of fermentation water, and the quality of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a green colistin extraction method. The key is that the method comprises the following steps of (a) heating colistin fermentation liquor; (b) adjusting the pH value to 1.5-2.0 by using an acidic substance, and then performing stirring for 30 minutes; (c) adjusting the pH value to 9.5-11.5 by using a divalent alkaline substance, and then performing stirring for 30 minutes; (d) performing plate frame filtration, collecting solids, neutralizing a filtrate, and taking the neutralized filtrate as water for fermentation of the next batch of colistin; (e) dissolving the solids obtained by the plate frame filtration with an acidic solution; (f) adding activated carbon and a purifying agent into an acidic dissolving solution, and performing plate frame filtration to obtain a colistin concentrated solution; (g) carrying out resin desalination and ultrafiltration on the concentrated solution to remove endotoxin, and finally carrying out spray drying to obtain a finished product; and (h) applying a trapped fluid of an ultrafiltration membrane to the next batch of colistin fermentation liquor for dilution. The extraction method has the advantages of short process, low sewage treatment cost and less environmental pollution.

Description

Technical field[0001]The invention belongs to the technical field of medicine, and specifically relates to a method for extracting colistin in a green color.Background technique[0002]At present, the industry mainly uses the ion exchange resin method to extract colistin. The pentavalent colistin cation is adsorbed through the macroporous resin, and then the colistin is eluted from the resin with sulfuric acid. Further, metal ions such as calcium and magnesium in the material liquid are removed by desalting resin, and then decolorizing agent is used for decolorizing treatment, and finally spray-dried to obtain finished powder. Although the quality of the product obtained by this method is high and stable, the adsorption effluent of the macroporous resin contains a large amount of impurities and has a large volume. Finally, it is discharged as wastewater, which puts huge pressure on cost and environmental protection.[0003]Other separation processes of colistin mainly include solvent ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/62C07K1/36C07K1/34C07K1/16C07K1/14
CPCC07K7/62
Inventor 常国栋王绘砖徐珍袁昉
Owner HEBEI SHENGXUE DACHENG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com