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Preparation of concentrated growth factor kits derived from umbilical cord blood

A technology of growth factors and kits, which is applied in the field of preparation of concentrated growth factor kits derived from umbilical cord blood, and can solve problems such as affecting activity

Active Publication Date: 2021-05-18
无锡创盟精准医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention proposes a novel method for preparing a concentrated growth factor kit derived from umbilical cord blood for the problem that an anticoagulant needs to be added to the preparation of the concentrated growth factor kit from the traditional cord blood source, which affects its activity.

Method used

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  • Preparation of concentrated growth factor kits derived from umbilical cord blood
  • Preparation of concentrated growth factor kits derived from umbilical cord blood
  • Preparation of concentrated growth factor kits derived from umbilical cord blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Collection of Cord Blood

[0038] In this example, with the informed consent of the puerpera and family members, a 20 mL-50 mL disposable needle was used to collect umbilical cord blood from newborns in the third stage of labor. 20 mL-50 mL of umbilical cord blood was collected per bag, as follows:

[0039] a. First, prepare the combined cryoprotectant: prepare 30 minutes before cord blood storage, 12% hydroxyethyl starch (HES) + 10% AB serum + 10% dimethyl sulfoxide (DMSO) + 68RMPI1640 solution, mix well After homogenization, set aside at 4°C;

[0040] b. After disinfecting the iodophor cotton ball, clamp and cut the umbilical cord at 10-15 cm from the root of the neonatal umbilical cord with a hemostat, and cut it as close to the fetus as possible;

[0041] c. Quickly clean from the broken umbilical end to the placenta, and disinfect the umbilical cord near the fetal end twice with iodophor gauze;

[0042] d. Use a 20 mL syringe needle to puncture the distal end...

Embodiment 2

[0068] The kit in this example contains two 20 mL or 50 mL sterile disposable syringes, one blood collection needle, three Eppendorf tubes, three special blood collection tubes, and six 9 mL vacuum negative pressure centrifuge tubes (Germany Greiner Bio. One company, 3 white and 3 green).

[0069] The laboratory is also equipped with an incubator with ice cubes, a continuously variable speed centrifuge, and storage of CGF blood collection tubes. The specific process is as follows:

[0070] (1) Collect umbilical cord blood, one for every 20 mL according to the course of treatment, and transport it to the preparation laboratory in a liquid nitrogen tank or dry ice. The laboratory complies with GMP standards, and the operation process is under sterile conditions.

[0071] (2) CGF adopts the differential speed uninterrupted centrifugation technology, using Italian centrifugal accelerator (Silfradent, Italy, centrifugal radius 17.5 cm.), the specific procedure is as follows: centri...

Embodiment 3

[0075] The CGF produced by the kit of this example can be stored in large quantities for a long time, and can be pressed into a film for auxiliary healing of clinical wound surfaces, and can also be shredded and mixed with biological materials for tissue regeneration without causing any infection. The samples preserved at low temperature greatly retain the integrity of the samples, and they are thawed in advance, and the samples are well preserved when they are revived in a water bath.

[0076] The invention perfects and fills up the gap in the field of transportation and storage in the field of blood separation, and is a technology to achieve the purpose of production and preparation by using a kit. The technology of the present invention realizes large-scale preparation and large-scale storage through dry and freeze-preservation technology, reduces the repeated collection of clinical patients in multiple treatment stages, and effectively avoids the injury and psychological pr...

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Abstract

The invention belongs to the technical field of biological kit preparation, and relates to a preparation method of a concentrated growth factor kit derived from umbilical cord blood. After the umbilical cord blood is collected, it is cryopreserved and transported by adding a cryoprotectant, after rewarming, it is centrifuged at a differential speed, and the functional laminated film is obtained. The invention perfects and fills up the gap in the field of transportation and storage in the field of blood separation, and is a technology to achieve the purpose of production and preparation by using a kit. The technology of the present invention realizes large-scale preparation and large-scale storage through dry and freeze-preservation technology, reduces the repeated collection of clinical patients in multiple treatment stages, and effectively avoids the injury and psychological pressure brought by secondary trauma to users.

Description

technical field [0001] The invention belongs to the technical field of biological kit preparation, and relates to a preparation method of a concentrated growth factor kit derived from umbilical cord blood. Background technique [0002] The research on plasma extract has roughly gone through three stages: platelet-rich plasma (Platelet-rich plasma, PRP), platelet-rich fibrin (Platelet-rich fibrin, PRF), and concentrated growth factor (Concentrate GrowthFactors, CGF). Enzymes and animal serum, and the second centrifugation method, are easily contaminated and sensitized, and are facing elimination. Compared with CGF, the results of scanning electron microscopy show that collagen fibers of different diameters can be arranged in a three-dimensional network structure on the upper part of platelet-rich fibrin and concentrated growth factors, and a large number of granular platelets can be seen attached to the collagen fibers without cell structure; platelet-rich Collagen fibers in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0252
Inventor 李增芬谭汇泉
Owner 无锡创盟精准医学科技有限公司
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