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Direct writing type 3D printing bio-ink and preparation method thereof

A 3D printing and bio-ink technology, applied in the field of biomedicine, can solve problems such as difficulty in maintaining its own shape, poor stability of cellulose nanofibers, and easy dispersion

Active Publication Date: 2021-01-05
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cellulose nanofibers have poor stability and are easily dispersed in water, so it is difficult for structures printed with cellulose nanofibers to maintain their own shape in a liquid environment.

Method used

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  • Direct writing type 3D printing bio-ink and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A direct-writing 3D printing bio-ink, which is composed of cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel and FBS-containing biological cell DMEM complete medium suspension, in mass percent, The proportion of cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel and FBS-containing DMEM complete medium suspension of biological cells is as follows:

[0046] Cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel 60-90%;

[0047] Biological cell DMEM complete medium resuspension containing FBS 10-40%.

[0048] Among them, the biological cells used in the resuspension of biological cell DMEM complete medium containing FBS are mouse C2C12 myoblasts or mouse MC3T3 osteoblasts, both of which were purchased from the Cell Bank of the Chinese Academy of Sciences. DMEM complete medium and FBS are commonly used reagents for cell culture medium and were purchased from Gibco, USA. It should be noted that the biological cells used in the FBS-contai...

Embodiment 2

[0060] A kind of direct writing molding 3D prints biological ink, and other features are identical with embodiment 1, and difference is: by mass percent, cellulose nanofiber-dopamine methacrylate 2-aminoethyl ester hydrogel and FBS-containing The ratio of biological cell DMEM complete medium resuspension solution is:

[0061] Cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel 70-80%;

[0062] Biological cell DMEM complete medium resuspension containing FBS 20-30%.

[0063] In terms of mass percent, the cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel contains:

[0064] Cellulose nanofiber-dopamine 2-aminoethyl methacrylate 3-7%;

[0065] LAP initiator 0.15~0.35%;

[0066] PBS buffer 92.65~96.85%.

[0067] Cellulose nanofibers, 2-aminoethyl methacrylate hydrochloride, dopamine, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide The mixing ratio is 1:3~7:3~7:5~17:5~17.

[0068] The direct-writing 3D printing bioi...

Embodiment 3

[0070] A kind of direct writing molding 3D prints biological ink, and other features are identical with embodiment 1, and difference is: by mass percent, cellulose nanofiber-dopamine methacrylate 2-aminoethyl ester hydrogel and FBS-containing The ratio of biological cell DMEM complete medium resuspension solution is:

[0071] Cellulose Nanofibers-Dopamine 2-Aminoethyl Methacrylate Hydrogel 60%;

[0072] Biological cell DMEM complete medium resuspension containing FBS 40%.

[0073] In terms of mass percent, the cellulose nanofiber-dopamine 2-aminoethyl methacrylate hydrogel contains:

[0074] Cellulose Nanofibers - Dopamine 2-Aminoethyl Methacrylate 1%;

[0075] LAP initiator 0.05%;

[0076] PBS buffer 98.95%.

[0077] Cellulose nanofibers, 2-aminoethyl methacrylate hydrochloride, dopamine, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide The mixing ratio is 1:1:1:2:2.

[0078] The direct-writing 3D printing bioink uses cellulose nanofib...

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Abstract

The invention provides a direct writing type 3D printing bio-ink and a preparation method thereof. The direct writing type 3D printing bio-ink is formed by mixing cellulose nanofiber -dopamine 2-aminoethyl methacrylate hydrogel and FBS-containing biological cell DMEM complete culture medium resuspension, and is prepared from the following components in percentage by mass: the ratio of cellulose nanofiber -dopamine 2-amino ethyl methacrylate hydrogel to FBS-containing biological cell DMEM complete culture medium resuspension is as follows: 60%-90% of the cellulose nanofiber-dopamine 2-amino ethyl methacrylate hydrogel and 10%-40% of the FBS-containing biological cell DMEM complete culture medium resuspension. The preparation method of the direct-writing type 3D printing bio-ink is simple and easy to implement, and the prepared direct-writing type 3D printing bio-ink has good biocompatibility, can promote cell adhesion and can be light-cured, so that the prepared 3D printing structure has good stability.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a direct-writing 3D printing bio-ink and a preparation method thereof. Background technique [0002] Direct writing molding 3D printing technology is a branch of 3D printing technology. It is a technology that precisely forms ink materials with specific components according to the structure set by computer software. Specifically, ink materials installed in the Z-axis direction are placed on the X-Y platform. Mobile extrusion forms the required graphics, selects the appropriate curing process for the graphics to cure and shape, and then prepares complex three-dimensional structures by layer-by-layer printing. The ink material used in direct writing 3D printing technology needs to have suitable rheological properties and shape retention. The rheology ensures that it can be extruded smoothly, and the shape retention ensures that the basic shape structure can be maintained after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/38C08B15/06D01F2/24B33Y70/10
CPCA61L27/20A61L27/3821A61L27/3826A61L2400/12B33Y70/10C08B15/06D01F2/24
Inventor 吴耀彬黄文华许益超利时雨
Owner SOUTHERN MEDICAL UNIVERSITY
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