A method for simultaneous detection of 9 chemical components in Lijunzi

A chemical composition, gentleman's technology, applied in the field of analysis and detection, can solve problems such as difficult to reflect the overall quality

Active Publication Date: 2022-06-03
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, at present, there are few research reports on the quality control of Shijunzi. The 2020 edition of "Pharmacopoeia of the People's Republic of China" (hereinafter referred to as "Chinese Pharmacopoeia") makes Trigonelline as the index for content determination of Junzi; Chen Zhihua et al. (Chen Zhihua, Li Menglu, Lv Weiqi, et al. Determination and correlation analysis of trigonelline content in the fruit of 18 producing areas of C. chinensis [J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2018, 28(11): 972-975.) HPLC method was used to determine the contents of C. chinensis in different producing areas Content of trigonelline in Chinese herbal medicine; Jiang Shunyao et al. (Jiang Shunyao, Tian Songjiu. Analysis of Trigonelline in Herbal Herbs by High Performance Ion Exchange Chromatography[J]. Chinese Journal of Traditional Chinese Medicine, 2004,29(2):44-46 +96.) Analysis of Trigonelline in Herbs of Junzi by High Performance Ion Exchange Chromatography
However, the above-mentioned prior art only detects the trigonelline component of Gentleman, which is difficult to reflect the overall quality of the various pharmacological effects of Gentleman.
At present, there is no literature report on the determination of the multi-components of Junzi, and the establishment of a multi-component comprehensive evaluation method is of great significance for improving the quality control of Junzi

Method used

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  • A method for simultaneous detection of 9 chemical components in Lijunzi
  • A method for simultaneous detection of 9 chemical components in Lijunzi
  • A method for simultaneous detection of 9 chemical components in Lijunzi

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

④: fenugreek, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid

and ferulic acid were 816.5ng / mL, 255.2ng / mL, 78.40ng / mL, 37.60ng / mL, 28.80ng / mL,

81.36ng / mL, 54.4ng / mL, 204.8ng / mL and 8.21ng / mL;

5.: fenugreek, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid

and ferulic acid were 3265.9ng / mL, 1020.8ng / mL, 313.6ng / mL, 150.40ng / mL, 115.2ng / mL

mL, 325.4ng / mL, 217.6ng / mL, 819.2ng / mL and 32.83ng / mL;

⑥: fenugreek, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid

and ferulic acid were 6531.8ng / mL, 2041.6ng / mL, 627.2ng / mL, 300.8ng / mL, 230.4ng / mL,

650.9ng / mL, 435.2ng / mL, 1638.4ng / mL and 65.66ng / mL

⑦: fenugreek, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid

and ferulic acid concentrations were 8164.8ng / mL, 2552.0ng / mL, 784.0ng / mL, 376.0ng / mL, 288.0ng / mL,

813.6 ng / mL, 544.0 ng / mL, 2048.0 ng / m...

Embodiment 1

[0127] 0.0~2.0min, the volume percentage of the mobile phase A is 2%;

[0128] 2.0~4.0min, the volume percentage of the mobile phase A is increased to 40% by a uniform speed of 2%;

[0129] 4.0~6.0min, the volume percentage of the mobile phase A is increased to 60% from 40% uniformly;

[0130] 6.0~6.5min, the volume percentage of the mobile phase A is reduced to 2% by a uniform speed of 60%;

[0131] 6.5 to 8.0 min, the volume percentage of the mobile phase A is 2%.

[0136]

[0141]

[0147]

Embodiment 2

[0150] Precision Test

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Abstract

The invention provides a method for simultaneously detecting 9 chemical components in genus chinensis, and relates to the technical field of analysis and detection. The present invention adopts the alcohol aqueous solution to extract the genus genus to obtain the sample liquid to be tested; and then adopts UPLC-MS / MS. The present invention uses the UPLC-MS / MS method to perform pretreatment on the sample of the genus chinensis to be tested, and controls the gradient elution conditions while quickly realizing qualitative identification and quantitative detection of 9 chemical species (trigonelline, vanillic acid, Ferulic acid, syringic acid, catechin, ellagic acid, malic acid, gallic acid, methyl gallate) provide reliable experimental data for the development, utilization and quality evaluation of Junzi medicinal materials. The detection method provided by the invention is simple to operate, only takes 8 minutes for rapid analysis, has a fast separation speed and high sensitivity, and fills up the gaps in the current nine chemical detection methods in Shijunzi, and is worthy of popularization and application.

Description

A method for simultaneous detection of 9 chemical components in the genitalia technical field The present invention relates to the technical field of analysis and detection, be specifically related to a kind of simultaneous detection of 9 kinds of chemical compositions in the gentleman method. Background technique To make a gentleman is to make the dry ripe fruit of the Gentlemen of the Gentlemen family to make the plant of the genus Gentleman to make the gentleman, and the nature is sweet and warm, and it returns to the stomach and the spleen meridian. Indications: killing insects and eliminating accumulation, invigorating the spleen and stomach, eliminating deficiency and heat, and killing worms. Modern pharmacological studies have shown that the gentleman has antibacterial, antiviral, and antibacterial properties. Deworming, antioxidant, anti-inflammatory, anti-hyperlipidemia, anti-diabetic, anti-cancer and other pharmacological effects. Research on chemical const...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06Y02A50/30
Inventor 许文温秀萍徐伟
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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