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Composite microbial agent for efficiently degrading high-grease kitchen garbage and preparation method of composite microbial agent

A compound microbial agent and kitchen waste technology, applied in the field of environmental protection, can solve problems such as complex process, secondary pollution by chemical methods, and high cost

Active Publication Date: 2020-12-22
深圳市嘉佳环保技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the treatment of this kind of high-fat kitchen waste, people have adopted different methods, such as physical methods such as sieve ratio, sedimentation, and oil separation, and chemical methods such as adsorption, electrolysis, and flocculation. These methods have the defects of complicated process and high cost, and Chemical methods also produce secondary pollution

Method used

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  • Composite microbial agent for efficiently degrading high-grease kitchen garbage and preparation method of composite microbial agent

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Effect test

Embodiment 1

[0025] The preparation of embodiment 1 bacterial liquid

[0026] The preparation method of the Bacillus licheniformis powder provided in this embodiment comprises the steps of: placing the Bacillus licheniformis mother liquor in the following activation medium for activation culture, and the activation medium, by weight percentage, includes: 5% peptone , yeast extract 8%, glucose 1.5%, cornstarch 0.5%, sesame oil 0.5%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.2%, water balance, pH value is 7.0-7.5; Cultivate in the primary enrichment medium for 8 hours, the primary enrichment medium is: peptone 0.5%, yeast extract 0.5%, glucose 1%, sesame oil 1%, sodium chloride 0.5%, water balance, pH value 7.0 to 7.5; add the cultured bacterial liquid to the secondary medium and cultivate it for 18 hours to obtain a Bacillus licheniformis liquid. The secondary medium is: 0.5% peptone, 0.8% yeast extract, 0.5% glucose, and 1.5% sesame oil , sodium chloride 0.5%, water balance...

Embodiment 2

[0035] The preparation method of the compound microbial bacterial agent for efficiently degrading high-fat kitchen waste provided by the present invention comprises the following steps:

[0036] (1) Take the following raw materials in parts by weight: 12 parts of Aspergillus oryzae liquid, 8 parts of Bacillus licheniformis liquid, 10 parts of Candida lipolytica liquid, 10 parts of Acetobacter, 15 parts of Bacillus coagulans, 12 parts of nitrogen-fixing bacterium brown balls; inoculate the raw material of the bacterial liquid into the fermentation medium with an inoculum of 4%, and at 30-35°C, under the condition that the ventilation volume is 1.1vvm and the pH value is 6.5-7.0, with Stirring and fermenting at a speed of 120r / min for 12h, stirring and fermenting at a speed of 180r / min for 48h under the conditions of an aeration rate of 1.4vvm and a pH value of 7.0 to 7.5, centrifuged and freeze-dried to obtain a composite bacterial powder, wherein the Said fermentation medium, ...

Embodiment 3

[0039] The preparation method of the compound microbial bacterial agent for efficiently degrading high-fat kitchen waste provided by the present invention comprises the following steps:

[0040] (1) Take the following raw materials in parts by weight: 24 parts of Aspergillus oryzae liquid, 12 parts of Bacillus licheniformis liquid, 15 parts of Candida lipolytica liquid, 20 parts of Acetobacter, 25 parts of Bacillus coagulans, 6 parts of brown spheroid nitrogen-fixing bacteria liquid; the raw material of the bacterial liquid was inoculated in the fermentation medium with an inoculum of 4%, and at 30-35°C, under the condition that the ventilation rate was 1.1vvm and the pH value was 6.5-7.0, the Stirring and fermenting at a speed of 120r / min for 12h, stirring and fermenting at a speed of 180r / min for 48h under the conditions of an aeration rate of 1.4vvm and a pH value of 7.0 to 7.5, centrifuged and freeze-dried to obtain a composite bacterial powder, wherein the Said fermentati...

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Abstract

The invention discloses a composite microbial agent for efficiently degrading high-grease kitchen garbage and a preparation method of the composite microbial agent. The composite microbial agent provided by the invention is prepared from aspergillus oryzae powder, bacillus licheniformis powder, candida lipolytica powder, bacillus aceticus powder, bacillus coagulans powder and azotobacter chroococcum powder. By researching compounding of various microorganisms, when the compounding ratio of the aspergillus oryzae powder to the bacillus licheniformis powder to the candida lipolytica powder to bacillus aceticus to bacillus coagulans to the azotobacter chroococcum powder is 2:1:1.2:1.5:2:1 and the inoculation amount is 4%, the degradation rate of the kitchen garbage can reach 92% or above, main components (cellulose, grease, starch and protein) of the kitchen garbage can be effectively degraded, and the kitchen garbage can be fully fermented.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a composite microbial agent for efficiently degrading high-grease kitchen waste and a preparation method thereof. Background technique [0002] Kitchen waste refers to the food waste and residues generated in the process of food processing and dining in households, catering industries, and canteens of enterprises and institutions. It is one of the main components of urban domestic waste. The traditional way of disposing of kitchen waste is to use landfill or incineration together with domestic waste. Landfill disposal of kitchen waste is likely to cause land pollution, and the landfill leachate spreads to the surrounding soil, flows into the groundwater body and causes water pollution. With the improvement of people's living standards and the increase of the catering industry, the grease content in the discharged kitchen waste is getting higher and higher. For ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14B09B3/00C12R1/69C12R1/10C12R1/73C12R1/02C12R1/07C12R1/065
CPCC12N1/20C12N1/16C12N1/14B09B3/00
Inventor 黄河唐勇
Owner 深圳市嘉佳环保技术有限公司
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