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A method and application of preparing white rot fungus liquid culture medium from tanning waste

A technology of liquid culture medium and white-rot fungus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of slow growth of microorganisms and affect the absorption of microorganisms, and achieve nutrient absorption and chromium content The effect of low, low chromium content

Active Publication Date: 2022-06-24
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under static conditions, the agar will form a nutrient-permeable barrier around the microbial cells during the cultivation process, affecting the nutrient absorption of the microorganisms, resulting in slow growth of the microorganisms

Method used

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  • A method and application of preparing white rot fungus liquid culture medium from tanning waste
  • A method and application of preparing white rot fungus liquid culture medium from tanning waste
  • A method and application of preparing white rot fungus liquid culture medium from tanning waste

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method of utilizing tannery waste to prepare a white rot fungal liquid culture medium, comprising the following steps:

[0038] (1) Hydrolysis and dechromization: in a constant temperature water bath at 60°C, add 25g of chrome leather scraps (containing Cr 2 O 3 : 3.2%), then add 3g of calcium oxide and 300g of deionized water, after stirring and reacting for 0.5h, add 12.5g of chrome leather scraps, continue to react for 0.5h, then add 12.5g of chrome leather scraps and continue to react for 1h to obtain a thick The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 min, discard the precipitate, a...

Embodiment 2

[0053] A method of utilizing tannery waste to prepare a white rot fungal liquid culture medium, comprising the following steps:

[0054] (1) Hydrolytic dechromization: in a constant temperature water bath at 60 °C, add 30 g of chrome leather scraps (containing Cr 2 O 3 : 2.5%), then add 4.2g of calcium oxide and 390g of deionized water, after stirring and reacting for 0.5h, add 15g discarded chrome leather scraps, continue the reaction for 0.5h, then add 15g chrome leather scraps and continue the reaction for 1h to obtain a thick The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 min, discard the precip...

Embodiment 3

[0069] A method of utilizing tannery waste to prepare a white rot fungal liquid culture medium, comprising the following steps:

[0070] (1) Hydrolytic dechromization: in a constant temperature water bath at 80°C, add 20g of chrome leather scraps (containing Cr 2 O 3 : 5%), then add 3.6g of calcium oxide and 400g of deionized water, after stirring for 0.5h, add 10g of chrome leather scraps, continue to react for 0.5h, then add 10g of chrome leather scraps and continue to react for 1h to obtain a viscous The hydrolyzed protein homogenate was subjected to centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 min, discard the precipitate, collect the liquid, measure th...

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Abstract

The present invention relates to a method and application for preparing white rot fungus liquid culture medium from tanning waste. Firstly, using tanning waste chromium leather shavings as raw material, and adopting batch-feeding process to prepare dechromed hydrolyzed collagen based on alkali-enzyme hydrolysis protein, and use tanning waste sawdust to prepare leachate. Secondly, using the extracted dechromed hydrolyzed collagen and sawdust extract as nutrient sources, glucose, peptone, trace element solution, and purified water were used to prepare a liquid fermentation medium; finally, the white rot fungus was cultivated using the liquid fermentation medium. The liquid culture medium prepared by the present invention can realize the normal and efficient growth of white rot fungi, not only realize the purpose of turning waste chromium leather shavings and sawdust from tanning into treasure, but also greatly replace traditional peptone resources, effectively reducing white rot The cost of cultivating fungi is conducive to the high-value utilization of chromium-containing solid waste.

Description

technical field [0001] The invention belongs to the field of leather solid waste, and in particular relates to a method and application for preparing a white rot fungus liquid culture medium from leather-making waste. Background technique [0002] In recent years, with the improvement of living standards, people's demand for diverse, beautiful and practical leather products has become more and more strong, which has also led to the rapid development of my country's tanning industry in the past three decades. Nowadays, leather products such as bags, leather shoes, leather bags, leather sofas, leather chairs, etc., which are dazzling, beautiful and yet unique, have long been inseparable from our lives. The traditional tanning process, from a macro perspective, is the process of converting the animal raw material skins discarded by animal husbandry into leather products with high use value and high artistic value, which is a recycling method of natural resources. It is the exi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P21/06C07K14/78C12R1/645
CPCC12N1/14C12P21/06C07K14/78
Inventor 刘洁张斐斐徐洲李彦春李雪松周玉
Owner QILU UNIV OF TECH
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