Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant polyploid induction method

A polyploid, plant technology, applied in the field of plant genetics and breeding, can solve the problem of low 2n gamete occurrence rate, and achieve the effects of ensuring normal growth and division, improving absorption, and increasing contact area.

Inactive Publication Date: 2020-12-18
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the growth points obtained by colchicine treatment are chimeras, and the incidence of 2n gametes induced by physical and chemical means is extremely low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant polyploid induction method
  • Plant polyploid induction method
  • Plant polyploid induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for inducing plant polyploidy: comprising the following steps:

[0033] S1, collection of explant anthers: in the inflorescence extraction and growth period of the rubber tree, choose to spray sulfur powder or spray triadimefon aerosol in the morning on a sunny day to prevent and treat powdery mildew. When the male flower is in a yellow-green state, take the male flower and crush it with tweezers, stain it with a nucleic acid dye, and observe the development period of the male flower under a microscope;

[0034] S2. Callus induction: use the anthers from the middle stage to the marginal stage of pollen grains as explants, inoculate them on medium A, and culture them in the dark at 20°C for 30 days to obtain callus tissue. Base A contains the following components: MS medium, 40mL / L coconut water, 50g / L sucrose, 1g / L plant gel, 0.3mg / L 2,4-dichlorophenoxyacetic acid, 0.5mg / L naphthaleneacetic acid, 0.3mg / L kinetin KT, 1ug / ml asiaticoside;

[0035] S3. Induction...

Embodiment 2

[0042]A method for inducing plant polyploidy: comprising the following steps:

[0043] S1, collection of explant anthers: in the inflorescence extraction and growth period of the rubber tree, choose to spray sulfur powder or spray triadimefon aerosol in the morning on a sunny day to prevent and treat powdery mildew. When the male flower is in a yellow-green state, take the male flower and crush it with tweezers, stain it with a nucleic acid dye, and observe the development period of the male flower under a microscope;

[0044] S2. Callus induction: use the anthers from the middle stage to the marginal stage of pollen grains as explants, inoculate them on medium A, and culture them in the dark at 30°C for 40 days to obtain callus tissue. Base A contains the following components: MS medium, 60mL / L coconut water, 90g / L sucrose, 3.2g / L plant gel, 1.5mg / L 2,4-dichlorophenoxyacetic acid, 1.8mg / L naphthaleneacetic acid , 1.5mg / L kinetin KT, 5ug / ml asiaticoside;

[0045] S3. Somatic...

Embodiment 3

[0052] A method for inducing plant polyploidy: comprising the following steps:

[0053] S1, collection of explant anthers: in the inflorescence extraction and growth period of the rubber tree, choose to spray sulfur powder or spray triadimefon aerosol in the morning on a sunny day to prevent and treat powdery mildew. When the male flower is in a yellow-green state, take the male flower and crush it with tweezers, stain it with a nucleic acid dye, and observe the development period of the male flower under a microscope;

[0054] S2. Callus induction: use the anthers from the middle stage to the marginal stage of pollen grains as explants, inoculate them on medium A, and culture them in the dark at 25°C for 35 days to obtain callus tissue. Base A contains the following components: MS medium, 50mL / L coconut water, 70g / L sucrose, 2.2g / L plant gel, 1mg / L 2,4-dichlorophenoxyacetic acid, 1mg / L naphthaleneacetic acid, 0.8 mg / L kinetin KT, 3ug / ml asiaticoside;

[0055] S3. Induction ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a plant polyploid induction method which comprises the following steps of explant anther collection, callus induction, somatic embryo induction, polyploid induction, regeneration plant induction and ploidy identification. According to the cultural method, on the basis of obtaining somatic embryos through in-vitro culture of anthers of rubber trees, a culture medium is scientifically prepared effectively, the culture conditions are reasonably adjusted, and a rubber tree polyploid with excellent properties can be quickly and efficiently obtained.

Description

technical field [0001] The invention relates to the field of plant genetics and breeding, in particular to a method for inducing plant polyploids. Background technique [0002] Natural rubber, a secondary metabolite of the rubber tree, is the main raw material for latex products and plays an important role in social and economic development. Plant polyploidy has the characteristics of huge cells, fast growth, strong stress resistance, and high content of secondary metabolites. Artificially inducing polyploidy in rubber trees is a potential way to improve the rubber production and stress resistance of rubber trees. [0003] At present, there are two main methods for artificially inducing polyploidy in rubber trees. One is to use colchicine to continuously treat the growth points (stem tips, bud points) of the plants to obtain tetraploid plants. The second is to process the developing male and female gametophytes of rubber trees by chemical (colchicine) or physical (high tem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 张源源黄肖王祥军李维国张晓飞高新生位明明
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com