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Stropharia rugosoannulata liquid strain incubator and culture method

A technology of liquid strains and stropharia, which is applied in biochemical equipment and methods, methods based on microorganisms, and methods of sampling biological materials, etc., can solve the problem of the large difference between the upper and lower vigor of the strains and the mycelium growth rate. Inconsistency, low efficiency of secondary liquid strains and other problems, to achieve the effect of shortening mycelium cultivation time, reducing cultivation cost, and low cost

Active Publication Date: 2020-12-15
ZHEJIANG JIAXING AGRI SCI ACADEMY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the secondary strains (original species) of stropharia are divided into solid strains and liquid strains. Solid strains are slow to germinate, and the vigor of the upper part of the strain is greatly different from that of the lower part. The inoculation operation is slow. The growth rate of mycelia is inconsistent; the production method of liquid strains generally adopts the technology of shaker and fermentation tank, and the efficiency of making secondary liquid strains by shaker is low, and the cost of fermentation tanks is high, and it is not easy to transport
The cultivation time of the tertiary species of Stropharia stropharia is long, and the growth rate of mycelium is about 2mm / d. In summer, the room temperature needs to be controlled at about 25°C, and it takes about 85 days to fill the bag with mycelium. The cost of cultivation is far greater than the cost of materials and labor.

Method used

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  • Stropharia rugosoannulata liquid strain incubator and culture method
  • Stropharia rugosoannulata liquid strain incubator and culture method
  • Stropharia rugosoannulata liquid strain incubator and culture method

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: parts by weight

[0044] Liquid mother seed culture medium: 4 parts of sawdust powder, 4 parts of glucose, 0.5 part of soybean meal, 992 parts of water;

[0045] Original seed culture medium: 9 parts of corn flour, 3 parts of sawdust powder, 9 parts of glucose, 2 parts of soybean meal, 977 parts of water;

[0046] Cultivated species nutrients: 38 parts of 3mm miscellaneous wood chips, 38 parts of 8mm miscellaneous wood chips; 7 parts of chaff, 4 parts of soybean meal, 5 parts of corn flour, 0.5 parts of light calcium carbonate, and 908 parts of water.

Embodiment 2

[0047] Embodiment 2: parts by weight

[0048] Liquid mother seed culture medium: 6 parts of sawdust powder, 6 parts of glucose, 1.5 parts of soybean meal, 986 parts of water;

[0049] Original seed culture medium: 11 parts of corn flour, 5 parts of sawdust powder, 11 parts of glucose, 4 parts of soybean meal, 969 parts of water;

[0050] Cultivated species nutrients: 42 parts of 3mm miscellaneous wood chips, 42 parts of 8mm miscellaneous wood chips; 9 parts of chaff, 6 parts of soybean meal, 7 parts of corn flour, 1.5 parts of light calcium carbonate, and 892 parts of water.

Embodiment 3

[0051] Embodiment 3: parts by weight

[0052] Liquid mother seed culture medium: 5 parts of sawdust powder, 5 parts of glucose, 1 part of soybean meal, 990 parts of water;

[0053] Original seed culture medium: 10 parts of corn flour, 4 parts of sawdust powder, 10 parts of glucose, 3 parts of soybean meal, 972 parts of water;

[0054] Nutrients for cultivating seeds: 40 parts of 3mm miscellaneous wood chips, 40 parts of 8mm miscellaneous wood chips; 8 parts of chaff, 5 parts of soybean meal, 6 parts of corn flour, 1 part of light calcium carbonate, and 900 parts of water.

[0055] Based on the formula in the above 3 embodiments, the cultivation of the liquid strain of Stropharia stropharia is carried out according to the following steps:

[0056] S1. Configure the liquid mother seed culture solution, divide it into multiple Erlenmeyer flasks, fill each bottle with 70ml culture solution, plug the bottle mouth with a silica gel plug with air holes, and put it in an autoclave fo...

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Abstract

The invention discloses a stropharia rugosoannulata liquid strain incubator. The stropharia rugosoannulata liquid strain incubator is characterized by comprising a liquid stock culture tank (1), a temperature control magnetic turntable (2) and a combined stirrer (3), wherein the liquid stock culture tank (1) is arranged above the temperature control magnetic turntable (2), stock culture liquid containing liquid mother strains is loaded in the liquid stock culture tank (1), and the combined stirrer (3) is placed in the stock culture liquid; and the temperature control magnetic turntable (2) rotates to drive the combined stirrer (3) to rotate, and non-contact stirring and heating of the stock culture liquid are achieved. When the incubator is used for strain culture, compared with solid stock culture, the stock culture time can be shortened by 40-60 days, and the spawn running time of cultivated species can be shortened by 30-50 days.

Description

technical field [0001] The invention belongs to the field of mushroom cultivation, in particular to a liquid strain cultivator and a cultivation method of Stropharia stropharia. Background technique [0002] Stropharia rugoso-annulata is also known as red matsutake and cloud mushroom. Its cap is wine red in color and the meat is crisp and tender. It is one of the top ten mushrooms in the international mushroom trading market and can consume a variety of agricultural and forestry products. Waste, the cultivation method is simple, and raw materials can be used for open field or facility cultivation, which is suitable for solving the problem of agricultural straw recycling and utilization caused by the prohibition of straw burning in my country. [0003] At present, the secondary strains (original species) of stropharia are divided into solid strains and liquid strains. Solid strains are slow to germinate, and the vigor of the upper part of the strain is greatly different from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/38C12M1/26C12M1/00C12N1/14C12R1/645
CPCC12M27/02C12M41/18C12M33/00C12N1/14Y02P60/21
Inventor 沈盟姚祥坦权新华袁晔王瑞森
Owner ZHEJIANG JIAXING AGRI SCI ACADEMY INST
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